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TECHNICAL ARTICLE
Year : 2010  |  Volume : 2  |  Issue : 12  |  Page : 598-601

Construction of shRNA lentiviral vector


Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Room L305, 50 Charlton Ave East, Hamilton, ON, Canada L8N 4A6
Canada
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Source of Support: None, Conflict of Interest: None


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Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.


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