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 Table of Contents  
ORIGINAL ARTICLE
Year : 2010  |  Volume : 2  |  Issue : 9  |  Page : 427-429

Post-natal maternal antiretroviral therapy and HIV prevalence among breast-fed infants in Benin, Nigeria


1 Department of Medical Microbiology, University of Benin Teaching Hospital, Benin City, Nigeria
2 School of Medical Laboratory Sciences, University of Benin Teaching Hospital P.M.B 1111, Benin City, Nigeria
3 Department of Microbiology, Faculty of Life Sciences, University of Benin, Benin City, Nigeria

Date of Web Publication9-Nov-2011

Correspondence Address:
Paul E Imade
Department of Medical Microbiology, University of Benin Teaching Hospital, Benin City
Nigeria
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Source of Support: None, Conflict of Interest: None


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  Abstract 

Background: Breastfeeding is an established mode of transmission of human immunodeficiency virus (HIV) infection resulting in clash between socio-cultural values and medical practice. Aims: This study aims to determine the effect of post-natal maternal antiretroviral therapy on transmission of HIV through breastfeeding. Patients and Methods: A total of 318 pregnant women were followed from pregnancy to 6 months post- partum. The women were divided into breast-fed and those who did not breast-feed, while the breast-fed were further divided into those on antiretroviral (ARV) and those not on ARV. After 6 months post-partum, dried blood spots were collected from infants born to these women and tested for HIV using polymerase chain reaction. Results: Generally, breast-feeding had 4 to 13 fold increase risk of transmission of HIV to infants (OR =7.079 95% CI = 3.768, 13.300; P <0.0001). However, among breast-fed infants, post-natal maternal ARV resulted in reduced prevalence of HIV compared to mothers who did not use ARV during breast-feeding (17.31% VS 92.00%; P<0.0001). Conclusion: The study demonstrates the effectiveness of post-natal maternal ARV. However, research into better feeding options to prevent mother to child transmission of HIV via breast-feeding is advocated.

Keywords: HIV, breast-feeding, antiretroviral therapy, infants, maternal.


How to cite this article:
Imade PE, Uwakwe NO, Omoregie R, Eghafona NO. Post-natal maternal antiretroviral therapy and HIV prevalence among breast-fed infants in Benin, Nigeria. North Am J Med Sci 2010;2:427-9

How to cite this URL:
Imade PE, Uwakwe NO, Omoregie R, Eghafona NO. Post-natal maternal antiretroviral therapy and HIV prevalence among breast-fed infants in Benin, Nigeria. North Am J Med Sci [serial online] 2010 [cited 2019 Nov 16];2:427-9. Available from: http://www.najms.org/text.asp?2010/2/9/427/86660


  Introduction Top


Breastfeeding is an integral part of a woman's motherhood and it is estimated that HIV transmission through breast-milk results in 200,000 HIV new cases yearly [1],[2] . It has already been reported that HIV can be transmitted through breast-feeding [1],[2],[3] . In resource-poor setting, it is difficult for mothers not to breast-feed their babies and one study has shown that mortality and morbidity rates were similar for both formula-fed and breast-fed infants [4] . Because of the stigma associated with HIV infection, most women choose to breast-feed to avoid the risk of revealing their HIV status. Many care givers are faced with these problems as conflicting reports exist on the benefits of exclusive breast-feeding, mixed feeding and formula feeding [5],[6],[7] .

The availability of highly active antiretroviral therapy (HAART) during pregnancy in combination with avoidance of breast-feeding and elective caesarian section has been reported to reduce or nearly eliminate mother to child transmission of HIV in developed countries [8] . A number of practices have been recommended by various researchers to reduce post-natal HIV transmission [1],[8] .

In our setting, exclusive formula feeding is recommended as a policy for HIV positive women. The stigma associated with HIV infection makes HIV infected women still breast-feed as avoiding breast-feeding may indicate a positive HIV status to her relatives or in-laws, thus bringing a clash between societal acceptance and medical practice. Against this background, this study is aimed at determining the effect of post-natal maternal antiretroviral therapy and breast-feeding on the prevalence of infant HIV infection.


  Patients and Methods Top


Study Area

This study was carried out in University of Benin Teaching Hospital, Benin City, Nigeria. The hospital is a tertiary hospital with a referral status and is one of the sites for Institute of Human Virology, Nigeria (IHVN) and the President Emergency plan for AIDS Relief (PEPFAR) HIV/AIDS intervention program in the country. HIV testing, treatment and monitoring are rendered at no cost and some of the patients were used in this study.

Study Population

A total of 318 HIV-positive pregnant women on their third trimester and on antiretroviral (ARV) drugs were recruited for this study. Each pregnant woman was counseled and informed about the study protocol. Verbal informed consent was obtained from each woman and her spouse prior to commencement of study.

Based on consent from the women and their spouses, the women were divided into two groups. Those that will exclusively breast-feed their babies and those that will not. This was further divided into those that will continue ARV and those that will not. The exclusive breast-feeding was for a period of 6 months after which infants born to these women were all screened for HIV using polymerase chain reaction (PCR). The protocol for this study was approved by the Ethical Committee of the University of Benin Teaching Hospital.

Collection and processing of samples

A drop of blood from finger prick of each infant was placed on sterile Whatman No 903 filter paper and allowed to dry as dried blood spot. The dried blood spots (DBS) collected from each infant were tested for HIV using a qualitative DNA polymerase chain reaction (PCR) (AMPLICOR ® HIV-1 DNA test version 1.5, Roche).

Statistical analysis

Data were analyzed using odd ratio analysis using the statistical software INSTAT® . A P value <0.05 was considered significant.


  Results Top


The prevalence of post-natal HIV infection was significantly higher (P<0.0001) in breast-fed infants compared with their non-breast-fed counterpart and breastfeeding was a risk factor for acquiring HIV infection among infants (OR=7.079 95%CI=3.268, 13.300; [Table 1]).
Table 1: Effect of Breast-feeding on prevalence of HIV among infants.

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The use of ARV during breastfeeding was not associated with post-natal HIV infection among infants (OR=0.018 95%CI=0.004, 0.091). None use of ARV during breastfeeding period was significantly (P<0.0001) associated with post-natal HIV infection (OR=54.944 95%CI=10.938, 276.00; [Table 2]).
Table 2: Effect of antiretroviral usage and breast feeding on the prevalence of HIV among infants.

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  Discussion Top


Against the background of conflicts between societal pressure to breast-feed (with the risk of exposing one's status and thus stigmatization) and medical practice to reduce the prevalence of post-natal HIV among infants, this study focused on determining the effect of prolonged maternal antiretroviral therapy and breast-feeding on the prevalence of infant HIV infection in Benin City, Nigeria.

A total of 54 (16.98%) out of 318 infants born to HIV positive mothers were infected with the HIV virus. Women who breast-fed exclusively had approximately 4 to 13 fold increase ask of acquiring HIV infection compared to their non-breastfed counterpart. This finding is similar to earlier reports [9],[10] and substantiates the policy in our setting. However, a number of studies have indicated increased risk of infant morbidity and mortality associated with replacement feeding [3],[11] . Inability to afford infant formula and poor condition for preparation of replacement feeding has been suggested as possible reasons for the high morbidity and mortality among formula-fed infants [3] . Although the current practice in our setting may be beneficial in reducing mother to child transmission of HIV infection, it is important to note that infectious diseases and malnutrition are the primary causes of death during infancy and artificial feeding substantially increases children risk of illness and death [2] .

Considering breast-fed infants, women who were on ARV throughout the breast-feeding period had significantly lower prevalence of HIV infection among their infants compared to infants born to women who were not on ARV during the breast-feeding period. This shows a beneficial effect of ARV during breast-feeding period and indeed it has been reported that exclusively breast-fed infants acquire infection after 6 months, once all exclusive breast-feeding has ceased [9] . Although one may not generally say that exclusive breast-feeding of infants born to HIV infected mothers should be a general policy as the sample size for this study was small and an HIV prevalence of 17.3% was observed, the benefit of post maternal ARV is clear. Perhaps future studies that will combine breast-feeding, maternal ARV and neonatal antiretroviral therapy may be able to bridge societal pressure to breast-feed against medical practice to reduce mother to child transmission of HIV through breast-feeding. Kumwenda et al. [12] had suggested antiretroviral prophylaxis for infants throughout the breast-feeding period to reduce HIV transmission and increase HIV free survival.


  Conclusion Top


This study has shown a reduced infant HIV infection among exclusively breast-fed infants whose mothers were on ARV during the breast-feeding period, although generally, non breast-fed infants had lower prevalence of infants HIV infection. Research into new feeding and antiretroviral therapy patterns to resolve the conflict between societal demand and medical practice is advocated.


  Acknowledgement Top


We thank the Management of the University of Benin Teaching Hospital for permission to carry out this work.

 
  References Top

1.Gray GE, Saloojee H. Breast-feeding, antiretroviral prophylaxis, and HIV. New Engl J Med 2008; 359 (2): 189-191.  Back to cited text no. 1
    
2.Coutsoudis, A. Breast-feeding and HIV transmission. Nutr Res Rev 2001; 14:191-206.  Back to cited text no. 2
    
3.Phadke MA, Gadgil B, Bharucha KE, et al. Replacement-fed infants born to HIV-infected mothers in India have a high early postpartum rate of hospitalization. J Nutrition 2003; 133: 3153 - 3157.  Back to cited text no. 3
    
4.Mbari-Ngacha D, Nduati R, John G, et al. Morbidity and mortality in breast-fed and formula-fed infants of HIV-1 infected women. A randomized clinical trial JAMA. 2001; 286: 2413-2420.  Back to cited text no. 4
    
5.Iliff PJ, Piwoz EG, Tavengwa NV, et al. Early exclusive breast-feeding reduces the risk of postnatal HIV-1 transmission and increases HIV-free survival. AIDS 2005; 19 (7): 641-652.  Back to cited text no. 5
    
6.Coovadia HM, Rollins NC, Bland RM. Mother to child transmission of HIV-1 infection during exclusive breast-feeding in the first 6 months of life: an intervention cohort study. Lancet 2007: 369 (9567): 1107-1116.  Back to cited text no. 6
    
7.Kuhn L, Sinkala M, Kankasa C, et al. High uptake of Exclusive Breast-feeding and Reduced Early post-natal HIV Transmission. Plos One 2007; 2 (12): e1363.  Back to cited text no. 7
    
8.Tomwe-Gold B, Ekouevi DK, Viho I, et al. Antiretrovival Treatment and Prevention of Peripartum and Postnatal HIV Transmission in West Africa: Evaluation of a two- tiered approach. PLOS 2007; 4 (8): 1362-1373.   Back to cited text no. 8
    
9.Coutsoudis A, Pillay K, Spooner E, Tsai WY, Coovadia HM. Method of feeding and transmission of HIV-1 from mother to children by 15 months of age: prospective cohort study from Durban, South Africa. AIDS 2001; 15:379-387.  Back to cited text no. 9
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10.Nduati R, John G, Mbori-Ngacha D, et al. Effect of breastfeeding and formula feeding on transmission of HIV-1: a randomized clinical trial. JAMA 2000; 283: 1167-1174.  Back to cited text no. 10
[PUBMED]  [FULLTEXT]  
11.WHO Collaborative study Team on the Role of Breast-feeding on the prevention of infant Mortality. Effect of breast feeding on infant and child mortality due to infectious diseases in less developed countries: a pooled analysis. Lancet 2000; 355: 451-455.  Back to cited text no. 11
    
12.Kumwenda NI, Hoover DR, Mofenson LM, et al. Extended antiretroviral prophylaxis to reduce breast-milk HIV-1 transmission. New Engl J Med 2008; 359: 119-129.  Back to cited text no. 12
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  In this article
Abstract
Introduction
Patients and Methods
Results
Discussion
Conclusion
Acknowledgement
References
Article Tables

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