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ORIGINAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 5  |  Page : 231-234

Assessment of Prevalence of Non-tuberculous Mycobacteria in Archival Acid-fast Bacilli Positive Smear Slides by TaqMan Real-time PCR Assay


1 Cell and Molecular Research Center, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
2 Departments of Infectious Diseases, Zabol University of Medical Sciences, Zabol, Iran
3 Zahedan University of Medical Sciences, Zahedan, Iran

Correspondence Address:
Taghi Naserpour Farivar
Associated Professor of Microbiology, Cell and Molecular Research Center and Department of Microbiology, School of Medicine, Qazvin University of Medical Scences, Qazvin
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.95907

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Background: The emergence of non-tuberculous mycobacteria as clinically relevant pathogens has necessitated us for the study of these organisms in the context of their environment. Differentiation of Mycobacterium tuberculosis complex and non-tuberculous mycobacteria is important especially when we have a positive smear slide test result. Aim: In this study, we planned to survey the prevalence of tuberculosis and non-tuberculous mycobacteria among archival acid-fast bacilli positive smear slides. Materials and Methods: A number of 200 acid-fast bacilli positive smear slides were collected from different parts of Sistan and Baluchestan Province, the biggest province of Iran with the highest incidence of tuberculosis. The presence of mycobacterial IS6110 was evaluated in slides' scraped material by TaqMan real-time polymerase chain reaction assay. Results: The real-time polymerase chain reaction tests of archival acid-fast bacilli positive smear slides showed that 171 slides from 200 examined slides had M. tuberculosis DNA and in the remaining 29 examined slides, M. tuberculosis DNA was not found. Conclusion: Our findings showed that there was no M. tuberculosis DNA in 14.5% of archival AFB positive smear slides, and this finding necessitates us to reviewing our diagnostic and anti- tuberculous protocols.


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