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 Table of Contents  
TECHNICAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 9  |  Page : 429-434

Western blot: Technique, theory, and trouble shooting


Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Date of Web Publication14-Sep-2012

Correspondence Address:
Ping-Chang Yang
Department of Pathology & Molecular Medicine, McMaster University, Room T3303, 50 Charlton Ave East, Hamilton, ON
Canada
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.100998

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  Abstract 

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.

Keywords: Bio-medical research, protein, western blot


How to cite this article:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. North Am J Med Sci 2012;4:429-34

How to cite this URL:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. North Am J Med Sci [serial online] 2012 [cited 2019 May 20];4:429-34. Available from: http://www.najms.org/text.asp?2012/4/9/429/100998


  Introduction Top


Western blot is often used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labels antibodies specific to the protein of interest.

The unbound antibody is washed off leaving only the bound antibody to the protein of interest. The bound antibodies are then detected by developing the film. As the antibodies only bind to the protein of interest, only one band should be visible. The thickness of the band corresponds to the amount of protein present; thus doing a standard can indicate the amount of protein present. The paper will first describe the protocol for western blot, accompanied by pictures to help the reader and theory to rationalize the protocol. This will be followed by the theoretical explanation of the procedure, and in the later section, troubleshooting tips for common problems.

Technique

Cell lysis to extract protein

Protein can be extracted from different kind of samples, such as tissue or cells. Below is the protocol to extract proteins from adherent cells.

Adherent cells:

  1. Wash cells in the tissue culture flask or dish by adding cold phosphate buffered saline (PBS) and rocking gently. Discard PBS. (Tip: Keep tissue culture dish on ice throughout).
  2. Add PBS and use a cell scraper to dislodge the cells. Pipette the mixture into microcentrifuge tubes.
  3. Centrifuge at 1500 RPM for 5 minutes and discard the supernatant.
  4. Add 180 μL of ice cold cell lysis buffer with 20 μL fresh protease inhibitor cocktail. (Tip: If protein concentration is not high enough at the end, it is advised to repeat the procedure with a higher proportion of protease inhibitor cocktail).
  5. Incubate for 30 minutes on ice, and then clarify the lysate by spinning for 10 minutes at 12,000 RPM, at 4°C.
  6. Transfer supernatant (or protein mix) to a fresh tube and store on ice or frozen at -20°C or -80°C.
  7. Measure the concentration of protein using a spectrophotometer.
Sample preparation



  1. determine the volume of protein extract to ensure 50 μg in each well.
  2. Add 5 μL sample buffer to the sample, and make the volume in each lane equalized using double distilled H 2 O (dd H 2 O). Mix well. (Tip: Total volume of 15 μL per lane is suggested).
  3. Heat the samples with dry plate for 5 minutes at 100°C.


Gel preparation



  1. After preparing the 10% stacking gel solution, assemble the rack for gel solidification [Figure 1]. (Tip: 10% AP and TEMED solidify the solution; therefore, both gels can be prepared at the same time, if the abovementioned reagents are not added until the end).
  2. Add stacking gel solution carefully until the level is equal to the green bar holding the glass plates [Figure 2]. Add H 2 O to the top. Wait for 15-30 minutes until the gel turning solidified. (Tip: Using a suction pipette can make the process of adding the gel to the glass plate easier).
  3. Overlay the stacking gel with the separating gel, after removing the water. (Tip: It is better to tilt the apparatus and use a paper towel to remove the water).
  4. Insert the comb, ensuring that there are no air bubbles.
  5. Wait until the gel is solidified. (Tip: Solidification can be easily checked by leaving some gel solution in a tube).
Figure 1: Assembled rack for gel solidification

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Figure 2: Add gel solution using a transfer pipette

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Electrophoresis

  1. Pour the running buffer into the electrophorator [Figure 3].
  2. Place gel inside the electrophorator and connect to a power supply. (Tip: When connecting to the power source always connect red to red, and black to black).
  3. Make sure buffer covers the gel completely, and remove the comb carefully.
  4. Load marker (6 μL) followed by samples (15 μL) in to each well [Figure 4].
  5. Run the gel with low voltage (60 V) for separating gel; use higher voltage (140 V) for stacking gel [Figure 5]a and b.
  6. Run the gel for approximately an hour, or until the dye front runs off the bottom of the gel [Figure 6].
Figure 3: Add running buffer to the electrophorator

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Figure 4: Add samples and molecular marker to the gel, after removing the combs

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Figure 5: (a) Samples running through the stacking gel (lower voltage). (b): Samples running through the separating gel (higher voltage)

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Figure 6: Run the gel to the bottom of the electrophorator

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Electrotransfer

  1. Cut 6 filter sheets to fit the measurement of the gel, and one polyvinylidene fluoride (PDVF) membrane with the same dimensions.
  2. Wet the sponge and filter paper in transfer buffer, and wet the PDVF membrane in methanol.
  3. Separate glass plates and retrieve the gel.
  4. Create a transfer sandwich as follows:

    Sponge

    3 Filter Papers

    Gel PVDF

    3 Filter Papers

    (Tip: Ensure there are no air bubbles between the gel and PVDF membrane, and squeeze out extra liquid).
  5. Relocate the sandwich to the transfer apparatus, which should be placed on ice to maintain 4°C. Add transfer buffer to the apparatus, and ensure that the sandwich is covered with the buffer. Place electrodes on top of the sandwich, ensuring that the PVDF membrane is between the gel and a positive electrode [Figure 7].
  6. Transfer for 90 minutes [Figure 8]. (Tip: The running time should be proportional to the thickness of the gel, so this may be reduced to 45 minutes for 0.75 mm gels).
Figure 7: Transfer should be done on ice

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Figure 8: Membrane after transfer

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Blocking and antibody incubation

  1. Block the membrane with 5% skim milk in TBST* for 1 hour.
  2. Add primary antibody in 5% bovine serum albumin ( BSA) and incubate overnight in 4°C on a shaker [Figure 9].
  3. Wash the membrane with TBST for 5 minutes. Do this 3 times. (Tip: All washing and antibody incubation steps should be done on a shaker at room temperature to ensure even agitation).
  4. Add secondary antibody in 5% skim milk in TBST, and incubate for 1 hour.
  5. Wash the membrane with TBST for 5 minutes. Do this 3 times
  6. Prepare ECL mix (following the proportion of solution A and B provided by the manufacturer). Incubate the membrane for 1-2 minutes [Figure 10]. (Tip: Use a 1000 μL pipette to ensure that ECL covers the top and bottom of the membrane).
  7. Visualize the result in the dark room [Figure 11]. (Tip: If the background is too strong, reduce exposure time).
*, TBST: Tris-Buffered Saline Tween-20
Figure 9: Use a shaker to incubate the membrane with antibody

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Figure 10: Incubate the membrane with ECL mix using a 1000 μL pipette to help the process

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Figure 11: Use the cassette to expose the membrane in the dark room

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Recipe

  1. Dissolve the following in 800 ml of distilled H 2 O

    • 8.8 g of NaCl
    • 0.2g of KCl
    • 3g of Tris base
  2. Add 500ul of Tween-20
  3. Adjust the pH to 7.4
  4. Add distilled H 2 O to 1L
  5. Sterilize by filtration or autoclaving
Theory

Sample preparation

Cell lysates are the most common form of sample used for western blot. Protein extraction attempts to collect all the proteins in the cell cytosol. This should be done in a cold temperature with protease inhibitors to prevent denaturing of the proteins. Since tissue sample display a higher degree of structure, mechanical invention, such as homogenization, or sonication is needed to extract the proteins.

After extracting the protein, it is very important to have a good idea of the extract's concentration. This eventually allows the researcher to ensure that the samples are being compared on an equivalent basis. Protein concentration is often measured using a spectrophotometer. Using this concentration allows to measure the mass of the protein that is being loaded into each well by the relationship between concentration, mass, and volume.

After determining the appropriate volume of the sample, it is diluted into a loading buffer, which contains glycerol so that the samples sink easily into the wells of the gel. A tracking dye (bromophenol blue) is also present in the buffer allowing the researcher to see how far the separation has progressed. The sample is heated after being diluted into a loading buffer, in order to denature the higher order structure, while retaining sulfide bridges. Denaturing the high structure ensures that the negative charge of amino acids is not neutralized, enabling the protein to move in an electric field (applied during electrotransfer).

It is also very important to have positive and negative controls for the sample. For a positive control a known source of target protein, such as purified protein or a control lysate is used. This helps to confirm the identity of the protein, and the activity of the antibody. A negative control is a null cell line, such as β-actin, is used as well to confirm that the staining is not nonspecific.

Gel electrophoresis

Western blot uses two different types of agarose gel: stacking and separating gel. The higher, stacking gel is slightly acidic (pH 6.8) and has a lower acrylamide concentration making a porous gel, which separates protein poorly but allows them to form thin, sharply defined bands. The lower gel, called the separating, or resolving gel, is basic (pH 8.8), and has a higher polyacrylamide content, making the gel's pores narrower. Protein is thus separated by their size more so in this gel, as the smaller proteins to travel more easily, and hence rapidly, than larger proteins.

The proteins when loaded on the gel have a negative charge, as they have been denatured by heating, and will travel toward the positive electrode when a voltage is applied. Gels are usually made by pouring them between two glass or plastic plates, using the solution described in the protocol section. The samples and a marker are loaded into the wells, and the empty wells are loaded with sample buffer. The gel is then connected to the power supply and allowed to run. The voltage is very important, as a high voltage can overheat and distort the bands.

Blotting

After separating the protein mixture, it is transferred to a membrane. The transfer is done using an electric field oriented perpendicular to the surface of the gel, causing proteins to move out of the gel and onto the membrane. The membrane is placed between the gel surface and the positive electrode in a sandwich. The sandwich includes a fiber pad (sponge) at each end, and filter papers to protect the gel and blotting membrane [Figure 12]. Here two things are very important: (1) the close contact of gel and membrane to ensure a clear image and (2) the placement of the membrane between the gel and the positive electrode. The membrane must be placed as such, so that the negatively charged proteins can migrate from the gel to the membrane. This type of transfer is called electrophoretic transfer, and can be done in semi-dry or wet conditions. Wet conditions are usually more reliable as it is less likely to dry out the gel, and is preferred for larger proteins.
Figure 12: Assembly of a sandwich in western Blot

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The membrane, the solid support, is an essential part of this process. There are two types of membrane: nitrocellulose and PVDF. Nitrocellulose is used for its high affinity for protein and its retention abilities. However, it is brittle, and does not allow the membrane to be used for reprobing. In this regard, PVDF membranes provide better mechanical support and allow the blot to be reprobed and stored. However, the background is higher in the PVDF membranes and therefore, washing carefully is very important.

Washing, blocking and antibody incubation

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk diluted in TBST to reduce the background.

Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution. For example, BSA blocking solutions are preferred with biotin and AP antibody labels, and antiphosphoprotein antibodies, since milk contains casein, which is itself a phosphoprotein and biotin, thus interfering with the assay results. It is often a good strategy to incubate the primary antibody with BSA since it is usually needed in higher amounts than the secondary antibody. Putting it in BSA solution allows the antibody to be reused, if the blot does not give good result.

The concentration of the antibody depends on the instruction by the manufacturer. The antibody can be diluted in a wash buffer, such as PBS or TBST. Washing is very important as it minimized background and removes unbound antibody. However, the membrane should not be left to wash for a really long time, as it can also reduce the signal.

The membrane is then detected using the label antibody, usually with an enzyme such as horseradish peroxidase (HRP), which is detected by the signal it produces corresponding to the position of the target protein. This signal is captured on a film which is usually developed in a dark room.

Quantification

It is very important to be aware that the data produced with a western blot is typically considered to be semi-quantitative. This is because it provides a relative comparison of protein levels, but not an absolute measure of quantity. There are two reasons for this; first, there are variations in loading and transfer rates between the samples in separate lanes which are different on separate blots. These differences will need to be standardized before a more precise comparison can be made. Second, the signal generated by detection is not linear across the concentration range of samples. Thus, since the signal produced is not linear, it should not be used to model the concentration.

Troubleshooting

Even though the procedure for western blot is simple, many problems can arise, leading to unexpected results. The problem can be grouped into five categories: (1) unusual or unexpected bands, (2) no bands, (3) faint bands or weak signal, (4) high background on the blot, and (5) patchy or uneven spots on the blot.

Unusual or unexpected bands can be due to protease degradation, which produces bands at unexpected positions. In this case it is advisable to use a fresh sample which had been kept on ice or alter the antibody. If the protein seems to be in too high of a position, then reheating the sample can help to break the quaternary protein structure. Similarly, blurry bands are often caused by high voltage or air bubbles present during transfer. In this case, it should be ensured that the gel is run at a lower voltage, and that the transfer sandwich is prepared properly. In addition, changing the running buffer can also help the problem. Nonflat bands can be the result of too fast of a travel through the gel, due to low resistance. To fix this the gel should be optimized to fit the sample. Finally, white (negative) bands on the film are due to too much protein or antibody.

Another problem: no bands can also arise due to many reasons related to antibody, antigen, or buffer used. If an improper antibody is used, either primary or secondary, the band will not show. In addition, the concentration of the antibody should be appropriate as well; if the concentration is too low, the signal may not be visible. It is important to remember that some antibodies are not to be used for western blot. Another reason for no visible bands is the lowest concentration or absence of the antigen. In this case, antigen from another source can be used to confirm whether the problem lies with the sample or with other elements, such as the antibody. Moreover, prolonged washing can also decrease the signal. Buffers can also contribute to the problem. It should be ensured that buffers like the transfer buffer, TBST, running buffer and ECL are all new and noncontaminated. If the buffers are contaminated with sodium azide, it can inactivate HRP.

Similarly, weak signals can be caused by low concentration of antibody or antigen. Increasing exposure time can also help to make the band clearer. Another reason could be nonfat dry milk masking the antigen. In this case use BSA or decrease the amount of milk used.

High background is often caused by too high concentration of the antibody, which can bind to PVDF membranes. Another problem could be the buffers, which may be too old. Increasing the washing time can also help to decrease the background. Additionally, too high of an exposure can also lead to this problem. Therefore, it is advisable to check different exposure times to achieve an optimum time.

Patchy and uneven spots on the blot are usually caused by improper transfer. If there are air bubbles trapped between the gel and the membrane, it will appear darker on the film. It is also important to use a shaker for all incubation, so that there is no uneven agitation during the incubation. Once again, washing is of utmost importance as well to wash the background. This problem can also be caused by antibodies binding to the blocking agents; in this case another blocking agent should be tried. Filtering the blocking agent can also help to remove some contaminants. Finally, this problem can also be caused by aggregation of the secondary antibody; in this case, the secondary antibody should be centrifuged and filtered to remove the aggregated.


  Conclusion Top


Western blot is a technique that is very useful for protein detection as it allows the user to quantify the protein expression as well. This paper covered the protocol, the theory behind that protocol, and some troubleshooting techniques. Western blot can be seen as an intricate balance, as the researcher attempts to get a nonspecific, yet strong signal.


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9], [Figure 10], [Figure 11], [Figure 12]


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[Pubmed] | [DOI]
36 The novel protective effects of loganin against 1-methyl-4-phenylpyridinium-induced neurotoxicity: Enhancement of neurotrophic signaling, activation of IGF-1R/GLP-1R , and inhibition of RhoA/ROCK pathway
Yu-Ting Tseng,Wan-Jung Lin,Wan-Hsuan Chang,Yi-Ching Lo
Phytotherapy Research. 2018;
[Pubmed] | [DOI]
37 Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing
Daniel Hauser,Manuela Estermann,Ana Milosevic,Lukas Steinmetz,Dimitri Vanhecke,Dedy Septiadi,Barbara Drasler,Alke Petri-Fink,Vincent Ball,Barbara Rothen-Rutishauser
Nanomaterials. 2018; 8(12): 1065
[Pubmed] | [DOI]
38 Oxidative Stress-Protective and Anti-Melanogenic Effects of Loliolide and Ethanol Extract from Fresh Water Green Algae, Prasiola japonica
Sang Park,Eunju Choi,Sunggyu Kim,Dong Kim,Ji Kim,SeokGu Chang,Jae Choi,Kyung Park,Kyung-Baeg Roh,Jongsung Lee,Byong Yoo,Jae Cho
International Journal of Molecular Sciences. 2018; 19(9): 2825
[Pubmed] | [DOI]
39 The eNOS-NO pathway attenuates kidney dysfunction via suppression of inflammasome activation in aldosterone-induced renal injury model mice
Yuji Sogawa,Hajime Nagasu,Seiji Itano,Kengo Kidokoro,Shun’ichiro Taniguchi,Masafumi Takahashi,Hiroyuki Kadoya,Minoru Satoh,Tamaki Sasaki,Naoki Kashihara,David Long
PLOS ONE. 2018; 13(10): e0203823
[Pubmed] | [DOI]
40 Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations
Lucas Moreira dos Santos,Carolina Georg Magalhães,Paula de Lima Telmo,Michele Pepe Cerqueira,Rafael Amaral Donassolo,Fábio Pereira Leivas Leite,Guita Rubinsky Elefant,Luciana Farias da Costa Avila,Carlos James Scaini,Ângela Nunes Moreira,Fabricio Rochedo Conceição,Paulo Lee Ho
PLOS ONE. 2018; 13(12): e0208991
[Pubmed] | [DOI]
41 Evaluation of the renoprotective effect of nano turmeric against toxic dose of copper sulfate: Role of vascular cell adhesion molecule-1, kidney injury molecule-1, and signal transducer and activator of transcription 3 protein expressions
Bshayer Alharbi,Laila Fadda,Hanaa M Ali
Journal of Biochemical and Molecular Toxicology. 2018; : e22243
[Pubmed] | [DOI]
42 Comparison of iTRAQ and SWATH in a clinical study with multiple time points
Antti Jylhä,Janika Nättinen,Ulla Aapola,Alexandra Mikhailova,Matti Nykter,Lei Zhou,Roger Beuerman,Hannu Uusitalo
Clinical Proteomics. 2018; 15(1)
[Pubmed] | [DOI]
43 Leptin Modifies the Rat Heart Performance Associated with Mitochondrial Dysfunction Independently of Its Prohypertrophic Effects
Edna Berzabá-Evoli,Cecilia Zazueta,Jarumi Hishel Cruz Hernández,Nancy Patricia Gómez-Crisóstomo,Isela Esther Juárez-Rojop,Erick Natividad De la Cruz-Hernández,Eduardo Martínez-Abundis
International Journal of Endocrinology. 2018; 2018: 1
[Pubmed] | [DOI]
44 Effects of biphasic and monophasic electrical stimulation on mitochondrial dynamics, cell apoptosis, and cell proliferation
Maria R. Love,Jirapas Sripetchwandee,Siripong Palee,Siriporn C. Chattipakorn,Morton M. Mower,Nipon Chattipakorn
Journal of Cellular Physiology. 2018;
[Pubmed] | [DOI]
45 Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase
Neval Yaman Görük,Engin Deveci
Acta Cirurgica Brasileira. 2018; 33(7): 629
[Pubmed] | [DOI]
46 Impact of Mutations in the Hemagglutinin of H10N7 Viruses Isolated from Seals on Virus Replication in Avian and Human Cells
Viruses. 2018; 10(2): 83
[Pubmed] | [DOI]
47 High SMAD7 and p-SMAD2,3 expression is associated with environmental enteropathy in children
Sana Syed,Vincenzo Dinallo,Najeeha T. Iqbal,Laura Di Iorio,Davide Di Fusco,Shan Guleria,Beatrice C. Amadi,Kamran Sadiq,Christopher Moskaluk,S. Asad Ali,Paul Kelly,Giovanni Monteleone,Joseph M. Vinetz
PLOS Neglected Tropical Diseases. 2018; 12(2): e0006224
[Pubmed] | [DOI]
48 Chemi- and Bioluminescence of Cyclic Peroxides
Morgane Vacher,Ignacio Fdez. Galván,Bo-Wen Ding,Stefan Schramm,Romain Berraud-Pache,Pance Naumov,Nicolas Ferré,Ya-Jun Liu,Isabelle Navizet,Daniel Roca-Sanjuán,Wilhelm J. Baader,Roland Lindh
Chemical Reviews. 2018;
[Pubmed] | [DOI]
49 Microchannel device for proteomic analysis of migrating cancer cells
Loan Bui,Qionghua Shen,Tamara Hill,Sayem H Bhuiyan,Rami Barakat,Vanessa Saavedra,Calvin Kong,James D Battiste,Young-tae Kim
Biomedical Physics & Engineering Express. 2018; 4(6): 065026
[Pubmed] | [DOI]
50 Role of Dehydrin-FK506-binding proteins complex in enhancing drought tolerance through ABA-mediated signaling pathway
Poonam Tiwari,Yuvraj Indoliya,Pradyumna Kumar Singh,Poonam C. Singh,Puneet S. Chauhan,Veena Pande,Debasis Chakrabarty
Environmental and Experimental Botany. 2018;
[Pubmed] | [DOI]
51 How to make P-glycoprotein (ABCB1, MDR1) harbor mutations and measure its expression and activity in cell cultures?
Pablo Zubiaur,Miriam Saiz-Rodríguez,Dora Koller,María Carmen Ovejero-Benito,Aneta Wojnicz,Francisco Abad-Santos
Pharmacogenomics. 2018;
[Pubmed] | [DOI]
52 Analytical techniques for characterization of biological molecules – proteins and aptamers/oligonucleotides
Harleen Kaur,Sonali R Bhagwat,Tarun Kumar Sharma,Amit Kumar
Bioanalysis. 2018;
[Pubmed] | [DOI]
53 ??????????????? ???????? ??????????????? ??????????????? ????????????? ??????? ??? ?????? ??? ???????? ?????? ?????????????? ????????, "?????????"
?. ?. ???,?.?. ????????,?. ?. ????????,?.?. ?????????,?.?. ??????
Tsitologiya. 2018; (9): 704
[Pubmed] | [DOI]
54 A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery
Cláudia Sofia Ferreira Raposo de Magalhães,Marco Alexandre Cavaco Cerqueira,Denise Schrama,Márcio Júlio Vicente Moreira,Surintorn Boonanuntanasarn,Pedro Miguel Leal Rodrigues
Reviews in Aquaculture. 2018;
[Pubmed] | [DOI]
55 The role of posttranslational modifications of a-synuclein and LRRK2 in Parkinsonæs disease: Potential contributions of environmental factors
Edward Pajarillo,Asha Rizor,Jayden Lee,Michael Aschner,Eunsook Lee
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2018;
[Pubmed] | [DOI]
56 Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
Milena Radakovic,Suncica Borozan,Ninoslav Djelic,Saša Ivanovic,Dejana Cupic Miladinovic,Marko Ristanic,Biljana Spremo-Potparevic,Zoran Stanimirovic
Oxidative Medicine and Cellular Longevity. 2018; 2018: 1
[Pubmed] | [DOI]
57 Ameliorative role of ferulic acid against diabetes associated oxidative stress induced spleen damage
Sumit Ghosh,Sayantani Chowdhury,Poulami Sarkar,Parames C. Sil
Food and Chemical Toxicology. 2018; 118: 272
[Pubmed] | [DOI]
58 5-Methoxyindole-2-carboxylic acid (MICA) suppresses Aß-mediated pathology in C. elegans
Waqar Ahmad,Paul R. Ebert
Experimental Gerontology. 2018; 108: 215
[Pubmed] | [DOI]
59 Early Diagnosis of Disease Using Microbead Array Technology: A Review
Atieh Vafajoo,Azin Rostami,Sanam Foroutan Parsa,Reza Salarian,Navid Rabiee,Ghazal Rabiee,Mohammad Rabiee,Mohammadreza Tahriri,Daryoosh Vashaee,Lobat Tayebi,Michael R. Hamblin
Analytica Chimica Acta. 2018;
[Pubmed] | [DOI]
60 Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
Oluwapelumi O. Adeyemi,Lee Sherry,Joseph C. Ward,Danielle M. Pierce,Morgan R. Herod,David J. Rowlands,Nicola J. Stonehouse,Julie K. Pfeiffer
Journal of Virology. 2018; 93(5)
[Pubmed] | [DOI]
61 Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation
Hussaini Majiya,Oluwapelumi O. Adeyemi,Nicola J. Stonehouse,Paul Millner
Journal of Photochemistry and Photobiology B: Biology. 2018; 178: 404
[Pubmed] | [DOI]
62 Evaluation methods as quality control in the generation of decellularized peripheral nerve allografts
Charlot Philips,Maria Cornelissen,Víctor Carriel
Journal of Neural Engineering. 2018; 15(2): 021003
[Pubmed] | [DOI]
63 MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation
J. J. Brudvig,J. T. Cain,G. G. Schmidt-Grimminger,D. J. Stumpo,K. J. Roux,P. J. Blackshear,J. M. Weimer
Molecular Neurobiology. 2018;
[Pubmed] | [DOI]
64 A Case of Concomitant Pemphigus Foliaceus and Oral Pemphigus Vulgaris
Alexandra C. Perks,Paula M. Farthing,Ruth Murphy,Anne M. Hegarty
Head and Neck Pathology. 2018;
[Pubmed] | [DOI]
65 Analysis for Science Librarians of the 2017 Nobel Prize in Physiology or Medicine: The Life and Work of Jeffrey C. Hall, Michael Rosbash, and Michael W. Young
Neyda V. Gilman
Science & Technology Libraries. 2018; : 1
[Pubmed] | [DOI]
66 Comparison of untagged and his-tagged dihydrodipicolinate synthase from the enteric pathogen Vibrio cholerae
Ruchi Gupta,Tatiana P. Soares da Costa,Pierre Faou,Con Dogovski,Matthew A. Perugini
Protein Expression and Purification. 2018;
[Pubmed] | [DOI]
67 Expression, activation and processing of a novel plant milk-clotting aspartic protease in Pichia pastoris
Lucía Feijoo-Siota,José Luis R. Rama,Angeles Sánchez-Pérez,Tomás G. Villa
Journal of Biotechnology. 2018; 268: 28
[Pubmed] | [DOI]
68 Zinc stimulates glucose oxidation and glycemic control by modulating the insulin signaling pathway in human and mouse skeletal muscle cell lines
Shaghayegh Norouzi,John Adulcikas,Sukhwinder Singh Sohal,Stephen Myers,Chih-Hsin Tang
PLOS ONE. 2018; 13(1): e0191727
[Pubmed] | [DOI]
69 Assessment of TSPO in a Rat Experimental Autoimmune Myocarditis Model: A Comparison Study between [18F]Fluoromethyl-PBR28 and [18F]CB251
Ga Kim,Jin Paeng,Jae Jung,Byung Moon,Antonio Lopalco,Nunzio Denora,Byung Lee,Sang Kim
International Journal of Molecular Sciences. 2018; 19(1): 276
[Pubmed] | [DOI]
70 Identification of a novel member of the pH responsive pathway Pal/Rim in Ustilago maydis
Juan A. Cervantes-Montelongo,José Ruiz-Herrera
Journal of Basic Microbiology. 2018;
[Pubmed] | [DOI]
71 Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/ß-catenin and MAPK pathway
Hongpeng Yang,Yue Guo,Dawei Wang,Xiaofei Yang,Chengzhi Ha
Organogenesis. 2018; 14(1): 36
[Pubmed] | [DOI]
72 Identification of protective protein antigens for vaccination against Streptococcus dysgalactiae in cobia ( Rachycentron canadum )
Thuy Thi Thu Nguyen,Hai Trong Nguyen,Hung Vu-Khac,Pei-Chi Wang,Shih-Chu Chen
Fish & Shellfish Immunology. 2018; 80: 88
[Pubmed] | [DOI]
73 Plasmonic biosensor based on vertical arrays of gold nanoantennas
Stephanie Klinghammer,Tino Uhlig,Fabian Patrovsky,Matthias Böhm,Julian Schuett,Nils Pütz,Larysa Baraban,Lukas M. Eng,Gianaurelio Cuniberti
ACS Sensors. 2018;
[Pubmed] | [DOI]
74 The protective mechanism of Nigella sativa against diethylnitrosamine-induced hepatocellular carcinoma through its antioxidant effect and EGFR/ERK1/2 signaling
Y. R. Shahin,N. M. Elguindy,A. Abdel Bary,M. Balbaa
Environmental Toxicology. 2018;
[Pubmed] | [DOI]
75 Deoxycholic acid promotes development of gastroesophageal reflux disease and Barrettæs oesophagus by modulating integrin-av trafficking
David O. Prichard,Anne Marie Byrne,James O. Murphy,John V. Reynolds,Jacintha OæSullivan,Ronan Feighery,Brendan Doyle,Osama Sharaf Eldin,Stephen P. Finn,Aoife Maguire,Deirdre Duff,Dermot P. Kelleher,Aideen Long
Journal of Cellular and Molecular Medicine. 2017;
[Pubmed] | [DOI]
76 Intracranial self-stimulation and immobilization had different effects on neurite extension and the p38 MAPK pathway in PC12m3 cells
Yutaka Gomita,Satoru Esumi,Yoshihisa Kitamura,Hirotoshi Motoda,Toshiaki Sendo,Hidenori Sagara,Hiroaki Araki,Mitsunobu Mio,Shigeki Inoue,Yoshio Kano
Life Sciences. 2017; 190: 78
[Pubmed] | [DOI]
77 L- and T-type calcium channel blockers protect against the inhibitory effects of mipafox on neurite outgrowth and plasticity-related proteins in SH-SY5Y cells
Laís Silva Fernandes,Neife Aparecida G. Dos Santos,Guilherme Luz Emerick,Antonio Cardozo Dos Santos
Journal of Toxicology and Environmental Health, Part A. 2017; : 1
[Pubmed] | [DOI]
78 Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
Alberto Sinibaldi,Camilla Sampaoli,Norbert Danz,Peter Munzert,Frank Sonntag,Fabio Centola,Agostino Occhicone,Elisa Tremante,Patrizio Giacomini,Francesco Michelotti
Biosensors. 2017; 7(3): 33
[Pubmed] | [DOI]
79 Human Sirtuin 3 (SIRT3) deacetylates histone H3 lysine 56 to promote Nonhomologous end joining repair
Amrita Sengupta,Devyani Haldar
DNA Repair. 2017;
[Pubmed] | [DOI]
80 Telmisartan Activates PPARd to Improve Symptoms of Unpredictable Chronic Mild Stress-Induced Depression in Mice
Yingxiao Li,Kai-Chun Cheng,Keng-Fan Liu,Wen-Huang Peng,Juei-Tang Cheng,Ho-Shan Niu
Scientific Reports. 2017; 7(1)
[Pubmed] | [DOI]
81 DNA polymerase beta participates in DNA End-joining
Sreerupa Ray,Gregory Breuer,Michelle DeVeaux,Daniel Zelterman,Ranjit Bindra,Joann B. Sweasy
Nucleic Acids Research. 2017;
[Pubmed] | [DOI]
82 Expression of Aspergillus Niger N5-5 in E. coli and Purification and Identification of Products
Shuai Zhang,Yong Cao,Hao Cheng
Saudi Journal of Biological Sciences. 2017;
[Pubmed] | [DOI]
83 Attenuation of the Infiltration of Angiotensin II Expressing CD3+ T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia – A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist
Nemat Khan,Arjun Muralidharan,Maree T. Smith
Frontiers in Molecular Neuroscience. 2017; 10
[Pubmed] | [DOI]
84 Silencing of the pentose phosphate pathway genes influences DNA replication in human fibroblasts
Karolina Fornalewicz,Aneta Wieczorek,Grzegorz Wegrzyn,Robert Lyzen
Gene. 2017; 635: 33
[Pubmed] | [DOI]
85 Protective Effects of ?-Mangostin on 6-OHDA-Induced Toxicity in SH-SY5Y Cells
Yamaratee Jaisin,Piyanee Ratanachamnong,Chitraporn Kuanpradit,Watinee Khumpum,Sunit Suksamrarn
Neuroscience Letters. 2017;
[Pubmed] | [DOI]
86 Using SDS-PAGE gel fingerprinting to identify soft-bodied wood-boring insect larvae to species
Mark A OæNeill,Mia Denos,Daniel Reed
Pest Management Science. 2017;
[Pubmed] | [DOI]
87 Processing fixed and stored adipose-derived stem cells for quantitative protein array assays
Jessica S. Sadick,Eric M. Darling
BioTechniques. 2017; 63(6)
[Pubmed] | [DOI]
88 Short-term regular aerobic exercise reduces oxidative stress produced by acute high intraluminal pressure in the adipose microvasculature
Austin T. Robinson,Ibra S. Fancher,Varadarajan Sudhahar,Jing Tan Bian,Marc D. Cook,Abeer M. Mahmoud,Mohamed M. Ali,Masuko Ushio-Fukai,Michael D. Brown,Tohru Fukai,Shane A. Phillips
American Journal of Physiology-Heart and Circulatory Physiology. 2017; 312(5): H896
[Pubmed] | [DOI]
89 Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety
Karamallah Al-Yousuf,Carl A. Webster,Grant N. Wheeler,Francesca Baldelli Bombelli,Victoria Sherwood
Current Protocols in Toxicology. 2017; 73(1): 20.13.1
[Pubmed] | [DOI]
90 Cigarette smokers develop altered erythrocyte membrane composition: an investigation unmasking the role of membrane bound integral protein GLUT 1
Jyotirmoy Sikdar,Paromita Seal,Amartya Roy,Rajen Haldar
Free Radical Research. 2017; 51(4): 375
[Pubmed] | [DOI]
91 Development and application of an enzyme-linked immunosorbent assay (ELISA) using a soluble recombinant nucleoprotein for the detection of antibodies to avian influenza virus
Monezi Borzi Mariana,Rodrigues Silva Ketherson,de Fatima Silva Montassier Maria,Santos Fernando Filipe,de Lourdes Feres Tamanine Maria,Moreira dos Santos Romeu,Schirato de Oliveira Elisabete,Casagrande Mariguela Viviane,Diniz Lopes Priscila,Reischak Dilmara,Oliveira Mendonca Andre,Luis Luciano Renato,Jose Montassier Helio
African Journal of Microbiology Research. 2017; 11(18): 697
[Pubmed] | [DOI]
92 TNF-a exerts cytotoxic effects on multidrug resistant breast cancer MCF-7/MX cells via a non-apoptotic death pathway
Morteza Ghandadi,Javad Behravan,Khalil Abnous,Melika Ehtesham Gharaee,Fatemeh Mosaffa
Cytokine. 2017; 97: 167
[Pubmed] | [DOI]
93 Capsanthin Inhibits both Adipogenesis in 3T3-L1 Preadipocytes and Weight Gain in High-Fat Diet-Induced Obese Mice
Sung Jun Jo,Jeung Won Kim,Hye Ok Choi,Jung Hwan Kim,Hyung Joong Kim,Sun Hee Woo,Byung Hoon Han
Biomolecules & Therapeutics. 2017; 25(3): 329
[Pubmed] | [DOI]
94 Hexacosanol reduces plasma and hepatic cholesterol by activation of AMP-activated protein kinase and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice
Ji Hae Lee,Yaoyao Jia,Trung Thanh Thach,Yuri Han,Bobae Kim,Chunyan Wu,Yeonji Kim,Woo Duck Seo,Sung-Joon Lee
Nutrition Research. 2017;
[Pubmed] | [DOI]
95 Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation
Ramirez-Lee Manuel Alejandro,Martinez-Cuevas Pedro Pablo,Rosas-Hernandez Hector,Oros-Ovalle Cuauhtémoc,Bravo-Sanchez Mariela,Martinez-Castañon Gabriel Alejandro,Gonzalez Carmen
Nanomedicine: Nanotechnology, Biology and Medicine. 2017;
[Pubmed] | [DOI]
96 A Novel Egr-1-Agrin Pathway and Potential Implications for Regulation of Synaptic Physiology and Homeostasis at the Neuromuscular Junction
Ryen MacDonald,Sebastien Barbat-Artigas,Chulmin Cho,Huashan Peng,Jijun Shang,Ayman Moustaine,Salvatore Carbonetto,Richard Robitaille,Lorraine E. Chalifour,Hemant Paudel
Frontiers in Aging Neuroscience. 2017; 9
[Pubmed] | [DOI]
97 Autophagy enhancement is rendered ineffective in presence of a-synuclein in melanoma cells
Swapna Nandakumar,Bejoy Vijayan,Asha Kishore,Anoopkumar Thekkuveettil
Journal of Cell Communication and Signaling. 2017;
[Pubmed] | [DOI]
98 Semi-Automatic Analysis for Unidimensional Immunoblot Images to Discriminate Breast Cancer Cases Using Time Series Data Mining
Diana M. Sánchez-Silva,Héctor G. Acosta-Mesa,Tania Romo-González
International Journal of Pattern Recognition and Artificial Intelligence. 2017; : 1860004
[Pubmed] | [DOI]
99 A novel mouse model of anterior segment dysgenesis (ASD): conditional deletion ofTsc1disrupts ciliary body and iris development
Anna-Carin Hägglund,Iwan Jones,Leif Carlsson
Disease Models & Mechanisms. 2017; 10(3): 245
[Pubmed] | [DOI]
100 High concentration of trichlorfon (1mM) disrupts axonal cytoskeleton and decreases the expression of plasticity-related proteins in SH-SY5Y cells
Laís Silva Fernandes,Guilherme Luz Emerick,Rafaela Scalco Ferreira,Neife Aparecida G. dos Santos,Antonio Cardozo dos Santos
Toxicology in Vitro. 2017; 39: 84
[Pubmed] | [DOI]
101 Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice
Mirza Muhammad Fahd Qadir,Attya Bhatti,Muhammad Usman Ashraf,Mansur Abdullah Sandhu,Sidrah Anjum,Peter John
Inflammopharmacology. 2017;
[Pubmed] | [DOI]
102 Altered functional efficacy of hippocampal interneuron during epileptogenesis following febrile seizures
Yeon Hee Yu,Kahyun Lee,Dal Sik Sin,Kyung-Ho Park,Dae-Kyoon Park,Duk-Soo Kim
Brain Research Bulletin. 2017; 131: 25
[Pubmed] | [DOI]
103 Hypoglycemic and anti-inflammatory effects of Psacalium paucicapitatum corms infusions
D. Jasso De Rodríguez,L.C. García-Hernández,N.E. Rocha-Guzmán,M.R. Moreno-Jiménez,R. Rodríguez-García,M.L.V. Díaz-Jiménez,M.L. Flores-López,J.A. Villarreal-Quintanilla,F.M. Peña-Ramos,D.A. Carrillo-Lomelí
Industrial Crops and Products. 2017;
[Pubmed] | [DOI]
104 Proteomics and irritable bowel syndrome
Athanasios Tsigaridas,Ioannis S. Papanikolaou,Anna Vaiopoulou,Athanasios K. Anagnostopoulos,Nikos Viazis,George Karamanolis,Dimitrios G. Karamanolis,George T. Tsangaris,Gerasimos J. Mantzaris,Maria Gazouli
Expert Review of Proteomics. 2017; : 1
[Pubmed] | [DOI]
105 Inhibition of heat shock protein (Hsp) 90 potentiates the antiproliferative and pro-apoptotic effects of 2-(4'fluoro-phenylamino)-4H-1,3-thiazine[6,5-b]indole in A2780cis cells
Zuzana Solárová,Martin Kello,Lenka Varinská,Mariana Budovská,Peter Solár
Biomedicine & Pharmacotherapy. 2017; 85: 463
[Pubmed] | [DOI]
106 Toxin-Antitoxin Systems in Estuarine Synechococcus Strain CB0101 and Their Transcriptomic Responses to Environmental Stressors
David Marsan,Allen Place,Daniel Fucich,Feng Chen
Frontiers in Microbiology. 2017; 8
[Pubmed] | [DOI]
107 Fruit Peel Polyphenolic Extract-Induced Apoptosis in Human Breast Cancer Cells Is Associated with ROS Production and Modulation of p38MAPK/Erk1/2 and the Akt Signaling Pathway
Martin Kello,Lucia Kulikova,Janka Vaskova,Alexandra Nagyova,Jan Mojzis
Nutrition and Cancer. 2017; : 1
[Pubmed] | [DOI]
108 Quantitative proteome-based systematic identification of SIRT7 substrates
Chaohua Zhang,Zichao Zhai,Ming Tang,Zhongyi Cheng,Tingting Li,Haiying Wang,Wei-Guo Zhu
PROTEOMICS. 2017; 17(13-14): 1600395
[Pubmed] | [DOI]
109 The ENU-3 protein family members function in the Wnt pathway parallel to UNC-6/Netrin to promote motor neuron axon outgrowth in C. elegans
Roxana Oriana Florica,Victoria Hipolito,Stephen Bautista,Homa Anvari,Chloe Rapp,Suzan El-Rass,Alimohammad Asgharian,Costin N. Antonescu,Marie T. Killeen
Developmental Biology. 2017;
[Pubmed] | [DOI]
110 5-hydroxytryptamine1A receptor density in the hippocampus of patients with temporal lobe epilepsy is associated with disease duration
N. C. Fonseca,H. P. G. Joaquim,L. L. Talib,S. Vincentiis,W. F. Gattaz,K. D. Valente
European Journal of Neurology. 2017;
[Pubmed] | [DOI]
111 The Potentials and Pitfalls of Microarrays in Neglected Tropical Diseases: A Focus on Human Filarial Infections
Alexander Kwarteng,Samuel Ahuno
Microarrays. 2016; 5(3): 20
[Pubmed] | [DOI]
112 Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria
Uri S. Markakpo,Kwabena M. Bosompem,Mawuli Dzodzomenyo,Anthony Danso-Appiah,Edward E. Essuman,William K. Anyan,Mitsuko Suzuki,Judith K. Stephens,Isaac Anim-Baidoo,Richard H. Asmah,Michael F. Ofori,Parnor Madjitey,Jonas B. Danquah,Naa Adjeley Frempong,Kofi D. Kwofie,Michael Amoa-Bosompem,David Sullivan,Julius N. Fobil,Isabella A. Quakyi
Tropical Medicine & International Health. 2016;
[Pubmed] | [DOI]
113 An overview of technical considerations for Western blotting applications to physiological research
J. J. Bass,D. J. Wilkinson,D. Rankin,B. E. Phillips,N. J. Szewczyk,K. Smith,P. J. Atherton
Scandinavian Journal of Medicine & Science in Sports. 2016;
[Pubmed] | [DOI]
114 ING3 is associated with increased cell invasion and lethal outcome in ERG-negative prostate cancer patients
Amal Almami,Samar A. Hegazy,Arash Nabbi,Mohammed Alshalalfa,Asma Salman,Hatem Abou-Ouf,Karl Riabowol,Tarek A. Bismar
Tumor Biology. 2016;
[Pubmed] | [DOI]
115 Atorvastatin improves pathological changes in the aged kidney by upregulating peroxisome proliferator-activated receptor expression and reducing matrix metalloproteinase-9 and transforming growth factor-ß1 levels
Jiahui Zhao,Qingli Cheng,Ping Ye,Guang Yang,Sheng Liu,Qiangguo Ao,Yang Liu,Yazuo Hu
Experimental Gerontology. 2016; 74: 37
[Pubmed] | [DOI]
116 Sphingosine kinase inhibition ameliorates chronic hypoperfusion-induced white matter lesions
Ying Yang,Federico Torta,Ken Arai,Markus R. Wenk,Deron R. Herr,Peter T.-H. Wong,Mitchell K.P. Lai
Neurochemistry International. 2016;
[Pubmed] | [DOI]
117 3D tumor spheroids: an overview on the tools and techniques used for their analysis
Elisabete C. Costa,André F. Moreira,Duarte de Melo-Diogo,Vítor M. Gaspar,Marco P. Carvalho,Ilídio J. Correia
Biotechnology Advances. 2016; 34(8): 1427
[Pubmed] | [DOI]
118 Perturbation of redox balance after thioredoxin reductase deficiency interrupts autophagy-lysosomal degradation pathway and enhances cell death in nutritionally stressed SH-SY5Y cells
Pandian Nagakannan,Mohamed Ariff Iqbal,Albert Yeung,James A. Thliveris,Mojgan Rastegar,Saeid Ghavami,Eftekhar Eftekharpour
Free Radical Biology and Medicine. 2016; 101: 53
[Pubmed] | [DOI]
119 Affinity Pulldown of Biotinylated RNA for Detection of Protein-RNA Complexes
Amaresh Panda,Jennifer Martindale,Myriam Gorospe
BIO-PROTOCOL. 2016; 6(24)
[Pubmed] | [DOI]
120 Easy and Fast Western Blotting by Thin-Film Direct Coating with Suction
Chao-Yuan Liu,De-Chao Lu,Yi-Wei Jiang,Yi-Kuang Yen,Shih-Chung Chang,An-Bang Wang
Analytical Chemistry. 2016;
[Pubmed] | [DOI]
121 Influence of Clinically Relevant Mechanical Forces on Vascular Smooth Muscle Cells Under Chronic High Glucose: An In Vitro Dynamic Disease Model
Varun Chawla,Agneta Simionescu,Eugene M. Langan,Martine LaBerge
Annals of Vascular Surgery. 2016;
[Pubmed] | [DOI]
122 Characterization and functional analysis of fatty acid binding protein from the carcinogenic liver fluke, Opisthorchis viverrini
Jittiyawadee Sripa,Thewarach Laha,Banchob Sripa
Parasitology International. 2016;
[Pubmed] | [DOI]
123 Quantification of Confocal Images Using LabVIEW for Tissue Engineering Applications
Lauren Sfakis,Tim Kamaldinov,Melinda Larsen,James Castracane,Alexander Khmaladze
Tissue Engineering Part C: Methods. 2016; 22(11): 1028
[Pubmed] | [DOI]
124 Anti-diabetes and anti-obesity: A meta-analysis of different compounds
Najeeb Ullah,Kinza Hafeez,Samia Farooq,Amna Batool,Noreen Aslam,Marzia Hussain,Sohail Ahmad
Asian Pacific Journal of Tropical Disease. 2016; 6(9): 749
[Pubmed] | [DOI]
125 Neuroprotective Effect of Puerarin on Glutamate-Induced Cytotoxicity in Differentiated Y-79 Cells via Inhibition of ROS Generation and Ca2+ Influx
Ke Wang,Xue Zhu,Kai Zhang,Zhifeng Wu,Song Sun,Fanfan Zhou,Ling Zhu
International Journal of Molecular Sciences. 2016; 17(7): 1109
[Pubmed] | [DOI]
126 Inhibition of connective tissue growth factor attenuates paraquat-induced lung fibrosis in a human MRC-5 cell line
Min Huang,Huifang Yang,Lingqin Zhu,Honghui Li,Jian Zhou,Zhijun Zhou
Environmental Toxicology. 2015; : n/a
[Pubmed] | [DOI]
127 A DEVH-box RNA Helicase from Leishmania braziliensis is Associated to mRNA Cytoplasmic Granules
Raquel N. Afonso-Lehmann,Maria C. Thomas,Maria A. Santana-Morales,Daniel Déniz,Manuel C. López,Basilio Valladares,Enrique Martínez-Carretero
Protist. 2015; 166(4): 457
[Pubmed] | [DOI]
128 Increased HMGB1 and cleaved caspase-3 stimulate the proliferation of tumor cells and are correlated with the poor prognosis in colorectal cancer
Zhengxiang Zhang,Min Wang,Ling Zhou,Xiao Feng,Jin Cheng,Yang Yu,Yanping Gong,Ying Zhu,Chuanyuan Li,Ling Tian,Qian Huang
Journal of Experimental & Clinical Cancer Research. 2015; 34(1)
[Pubmed] | [DOI]
129 Considerations when quantitating protein abundance by immunoblot
Alicia A. McDonough,Luciana C. Veiras,Jacqueline N. Minas,Donna Lee Ralph
American Journal of Physiology-Cell Physiology. 2015; 308(6): C426
[Pubmed] | [DOI]
130 Elevated Phospholipid Transfer Protein in Subjects with Multiple Sclerosis
Roy A. Garvin
Journal of Lipids. 2015; 2015: 1
[Pubmed] | [DOI]
131 The Localization of Cytochrome P450s CYP1A1 and CYP1A2 into Different Lipid Microdomains Is Governed by Their N-terminal and Internal Protein Regions
Ji Won Park,James R. Reed,Wayne L. Backes
Journal of Biological Chemistry. 2015; 290(49): 29449
[Pubmed] | [DOI]
132 Monocyte Chemoattractant Protein-Induced Protein 1 (MCPIP1) Enhances Angiogenic and Cardiomyogenic Potential of Murine Bone Marrow-Derived Mesenchymal Stem Cells
Anna Labedz-Maslowska,Barbara Lipert,Dominika Berdecka,Sylwia Kedracka-Krok,Urszula Jankowska,Elzbieta Kamycka,Malgorzata Sekula,Zbigniew Madeja,Buddhadeb Dawn,Jolanta Jura,Ewa K. Zuba-Surma,Gangjian Qin
PLOS ONE. 2015; 10(7): e0133746
[Pubmed] | [DOI]
133 Aspalathin improves glucose and lipid metabolism in 3T3-L1 adipocytes exposed to palmitate
Sithandiwe E. Mazibuko,Elizabeth Joubert,Rabia Johnson,Johan Louw,Andrew R. Opoku,Christo J. F. Muller
Molecular Nutrition & Food Research. 2015; 59(11): 2199
[Pubmed] | [DOI]
134 Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation
Chih-Chun Lin,Ru-Wei Lin,Chih-Wei Chang,Gwo-Jaw Wang,Kuo-An Lai
Bioelectromagnetics. 2015; 36(7): 494
[Pubmed] | [DOI]
135 Cloning of the first human anti-JCPyV/VP1 neutralizing monoclonal antibody: epitope definition and implications in risk stratification of patients under natalizumab therapy
Roberta Antonia Diotti,Nicasio Mancini,Nicola Clementi,Giuseppe Sautto,Guisella Janett Moreno,Elena Criscuolo,Francesca Cappelletti,Petr Man,Eric Forest,Louise Remy,Simone Giannecchini,Massimo Clementi,Roberto Burioni
Antiviral Research. 2014;
[Pubmed] | [DOI]
136 Power of Proteomics in Linking Oxidative Stress and Female Infertility
Sajal Gupta,Jana Ghulmiyyah,Rakesh Sharma,Jacques Halabi,Ashok Agarwal
BioMed Research International. 2014; 2014: 1
[Pubmed] | [DOI]
137 Different Effects of antimitotic drug on mechanical behaviors of Cancer cytoskeleton: Investigating distinct Grades of "colon Cancer cell lines"
S.M. Seyedpour,M. Pachenari,M. Janmaleki,M. Alizadeh,H. Hosseinkhani
Journal of Biomechanics. 2014;
[Pubmed] | [DOI]
138 The future of whole-cell modeling
Derek N Macklin,Nicholas A Ruggero,Markus W Covert
Current Opinion in Biotechnology. 2014; 28: 111
[Pubmed] | [DOI]
139 Proteomics, oxidative stress and male infertility
Ashok Agarwal,Damayanthi Durairajanayagam,Jacques Halabi,Jason Peng,Monica Vazquez-Levin
Reproductive BioMedicine Online. 2014;
[Pubmed] | [DOI]
140 Effects of cerebrolysin on motor-neuron-like NSC-34 cells
Gerburg Keilhoff,Benjamin Lucas,Josephine Pinkernelle,Michael Steiner,Hisham Fansa
Experimental Cell Research. 2014; 327(2): 234
[Pubmed] | [DOI]
141 Cholera toxin suppresses expression of ubiquitin editing enzyme A20 and enhances transcytosis
Li, M.-Y. and Zhu, M. and Zhu, B. and Wang, Z.-Q.
Cellular Physiology and Biochemistry. 2013; 31(4-5): 495-504
[Pubmed]
142 Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: Investigating different grades of colon cancer cell lines
M. Pachenari,S.M. Seyedpour,M. Janmaleki,S. Babazadeh Shayan,S. Taranejoo,H. Hosseinkhani
Journal of Biomechanics. 2013;
[Pubmed] | [DOI]



 

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