Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Visit old site
Home Print this page Email this page Small font size Default font size Increase font size
Users Online: 1305
ORIGINAL ARTICLE
Year : 2013  |  Volume : 5  |  Issue : 2  |  Page : 95-101

Genetic diversity of Plasmodium falciparum field isolates in central Sudan inferred by PCR genotyping of Merozoite surface protein 1 and 2


1 Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
2 Faculty of Veterinary Medicine, Sudan University of Science and Technology, Sudan
3 Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan; Faculty of Medicine, Jazan University, Saudi Arabia

Correspondence Address:
Muzamil M Abdel Hamid
Institute of Endemic Diseases, University of Khartoum, P.O. Box 102, Khartoum, Sudan

Login to access the Email id

Source of Support: This work received financial support from DAAD, Germany., Conflict of Interest: None


DOI: 10.4103/1947-2714.107524

Rights and Permissions

Background : Characterization of Plasmodium falciparum diversity is commonly achieved by amplification of the polymorphic regions of the merozoite surface proteins 1 (MSP1) and 2 (MSP2) genes. Aims : The present study aimed to determine the allelic variants distribution of MSP1 and MSP2 and multiplicity of infection in P. falciparum field isolates from Kosti, central Sudan, an area characterized by seasonal malaria transmission. Materials and Methods : Total 121 samples (N = 121) were collected during a cross-sectional survey between March and April 2003. DNA was extracted and MSP1 and MSP2 polymorphic loci were genotyped. Results : The total number of alleles identified in MSP1 block 2 was 11, while 16 alleles were observed in MSP2 block 3. In MSP1, RO33 was found to be the predominant allelic type, carried alone or in combination with MAD20 and K1 types, whereas FC27 family was the most prevalent in MSP2. Sixty two percent of isolates had multiple genotypes and the overall mean multiplicity of infection was 1.93 (CI 95% 1.66-2.20). Age correlated with parasite density ( P = 0.017). In addition, a positive correlation was observed between parasite densities and the number of alleles (P = 0.022). Conclusion : Genetic diversity in P. falciparum field isolates in central Sudan was high and consisted of multiple clones.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2288    
    Printed71    
    Emailed0    
    PDF Downloaded468    
    Comments [Add]    
    Cited by others 14    

Recommend this journal