Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Visit old site
Home Print this page Email this page Small font size Default font size Increase font size
Users Online: 287
ORIGINAL ARTICLE
Year : 2015  |  Volume : 7  |  Issue : 9  |  Page : 397-402

Immunofluorescence patterns in selected dermatoses, including blistering skin diseases utilizing multiple fluorochromes


1 Department of Immunodermatology, Georgia Dermatopathology Associates, Atlanta, Georgia, USA
2 Department of Dermatology, Clinica El Poblado, Medellin, Colombia, South America, USA
3 Dermatopathology, Georgia Dermatopathology Associates, Atlanta, Georgia, USA

Correspondence Address:
Michael S Howard
Dermatopathology, Georgia Dermatopathology Associates, Atlanta, Georgia
USA
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.166219

Rights and Permissions

Background: Autoimmune vesiculobullous disorders represent a heterogeneous group of dermatoses whose diagnosis is made based on clinical history, histologic features, and immunopathologic features. The most commonly used techniques for the diagnosis of these diseases are direct and indirect immunofluorescence (DIF and IIF), including salt-split processing. NaCl split skin is used to determine the level of blister formation, and the localization of autoantibodies relative to the split. Classically, immunofluorescence has been performed with one fluorochrome in the diagnosis of autoimmune bullous skin diseases. Aims: To compare DIF and IIF of the skin, using a single fluorochrome versus multiple fluorochromes. Materials and Methods: We studied 20 autoimmune skin disease cases using fluorescein isothiocyanate (FITC) alone, in comparison to multiple fluorochromes (with or without DNA counterstaining). Results: The use of multiple fluorochromes helped to simultaneously visualize reactivity in multiple skin areas, in contrast to using FITC alone. Conclusions: Using multiple fluorochromes allows simultaneous labeling of two or more antigens within the same cell/or tissue section, assists in colocalization of unknown antigens with known molecules, and helps in ruling out "background" staining.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1490    
    Printed34    
    Emailed0    
    PDF Downloaded179    
    Comments [Add]    

Recommend this journal