Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Visit old site
Home Print this page Email this page Small font size Default font size Increase font size
Users Online: 341
ORIGINAL ARTICLE
Year : 2016  |  Volume : 8  |  Issue : 5  |  Page : 215-221

Histopathology and histomorphometric investigation of bisphenol a and nonylphenol on the male rat reproductive system


1 Department of Pharmacology, Babol University of Medical Sciences; Department of Cellular and Molecular, Biology Research Center, Babol University of Medical Sciences, Babol, Iran
2 Department of Anatomical Sciences, Babol University of Medical Sciences, Babol, Iran
3 Department of Pharmacology, Babol University of Medical Sciences, Babol, Iran
4 Department of Cellular and Molecular, Biology Research Center, Babol University of Medical Sciences, Babol, Iran

Correspondence Address:
Ali Akbar Moghadamnia
Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol
Iran
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.183012

Rights and Permissions

Background: Bisphenol A (BPA) and nonylphenol (NP) have harmful effects on the endocrine system of humans and animals. Aim: We sought to investigate the effect of three doses of BPA and NP on the reproductive parameters of rats. Materials and Methods: Adult Wistar male rats weighing 150–200 g were used for a consecutive 35-day study. BPA and NP were given as gavage in three doses (5, 25, and 125 μg/kg). At the end of the study, the rats were anesthetized and 2 ml blood sample was obtained from the auxiliary venous plexus for the assessment of sex hormone levels. The testes were removed and kept in 10% formalin for the histomorphometric and histopathologic analyses. Results: BPA and NP significantly decreased the body weight of the animals compared to the controls (P < 0.05). The seminiferous tubule diameter and thickness of the seminiferous epithelium were significantly decreased in the groups receiving BPA and NP compared to the control (P < 0.05). The number of seminiferous tubules in every experimental group increased, except for the highest dose of NP (125 μg/kg), which showed a significant difference compared to the controls (P < 0.05). The number of spermatocytes and spermatogonia (54.97 ± 5.824, 35.78 ± 3.956, respectively) in the group receiving NP (125 μg/kg) was significantly decreased compared to the other groups. Serum levels of luteinizing hormone, Follicle stimulating hormone, and testosterone did not show any significant change compared to the controls. Conclusion: Based on the results, all three doses of BPA and NP significantly produced weight loss, as well as destruction of the testis tissue and impairment of the spermatogenesis.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2160    
    Printed49    
    Emailed0    
    PDF Downloaded414    
    Comments [Add]    
    Cited by others 14    

Recommend this journal