Original Article
Cytotoxic T lymphocytes
mediate chronic inflammation of the nasal mucosa of patients with atypical
allergic rhinitis
Shuqi Qiu1, 2, 3,*, Yun Du4, 5,*,
Xiaobei Duan1, Xiaorui Geng1, Jianxiong Xie2,
Han Gao1, 2, 3, Ping-Chang Yang4
1Shenzhen
ENT Institute, 2Shenzhen Longgang Central Hospital, 3ENT
Hospital, Shenzhen, China.
4Department
of Pathology & Molecular Medicine, McMaster University, Hamilton, Canada.
5Department
of Pathology, Hebei Medical University, Shijiazhuang, China.
Citation:
Qiu S, Du Y, Duan X, Geng X, Xie J, Gao
H, Yang P-C. Cytotoxic T lymphocytes mediate
chronic inflammation of the nasal mucosa of patients with atypical allergic
rhinitis.
North Am
J Med Sci 2011; 3:
378-383.
doi:
10.4297/najms.2011.3378
* Authors equally
contributed to this work, and are the joined first authors.
Abstract
Background:
The prevalence of chronic rhinitis is increasing rapidly; its pathogenesis is to
be further understood; immune inflammation is one of the possible causative
factors. Antigen specific CD8+ T cells play a critical role in the induction of
chronic inflammation. Aims: This
study aimed to investigate the role of antigen specific CD8+ T cells in the
pathogenesis of chronic atypical allergic rhinitis.
Material and Methods: Nasal mucosal
epithelial surface scratching samples were obtained from patients with chronic
obstruction atypical allergic rhinitis. Exosomes were purified from the
scratching samples and examined by immune gold electron microscopy. The effect
of exosomes on modulating dendritic cell’s properties, the effect of exosome-pulsed
dendritic cells on naïve T cell differentiation and the antigen specific CD8+ T
cell activation were observed by cell culture models.
Results: Exosomes purified from
patients with chronic atypical allergic rhinitis carried microbial products,
Staphylococcal enterotoxin B (SEB), and airborne antigen, Derp1. Dendritic cells
pulsed by SEB/Derp1-carrying exosomes showed high levels of CD80, CD86 and the
major histocompatibility class I (MHCI). Exosome-pulsed dendritic cells could
induce the naïve CD3+ T cells to differentiate into CD8+ T cells. Upon the
exposure to a specific antigen, the CD8+ T cells released granzyme B and
perforin; more than 30% antigen specific CD8+ T cells proliferated.
Conclusions: Antigen specific CD8+ T
cells play an important role in the pathogenesis of chronic obstruction atypical
allergic rhinitis.
Keywords:
Rhinitis, CD8 T lymphocytes, Epithelium, Antigen specific response.
Correspondence to:
Dr. Shuqi Qiu, Shenzhen ENT Institute, Shenzhen Longgang Central
Hospital, Shenzhen, Guangzhou, China.
Tel. /Fax: 0755 2658 6018, Email: qiuqi66858@163.com;
or Dr. Ping-Chang Yang. Room T3303,