North American Journal of Medical Sciences

: 2010  |  Volume : 2  |  Issue : 12  |  Page : 598--601

Construction of shRNA lentiviral vector

Hong Song, Ping-Chang Yang 
 Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Room L305, 50 Charlton Ave East, Hamilton, ON, Canada L8N 4A6

Lentiviruses have been adapted as gene delivery vehicles. This article summarized shRNA lentiviral vector methods generally used in research laboratories. The main procedures of shRNA lentiviral vector include that (1) Target sequences screening and shRNA oligonucleotides designing, (2) insert designed oligonucleotides into lentiviral vectors, (3) using packaging cells to produce shRNA lentivirus, and (4) transducing target cells with shRNA lentivirus.

How to cite this article:
Song H, Yang PC. Construction of shRNA lentiviral vector.North Am J Med Sci 2010;2:598-601

How to cite this URL:
Song H, Yang PC. Construction of shRNA lentiviral vector. North Am J Med Sci [serial online] 2010 [cited 2020 Jul 13 ];2:598-601
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