North American Journal of Medical Sciences

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 6  |  Issue : 12  |  Page : 643--647

Spasmolytic and anti-spasmodic action of Trachyspermum ammi essence on rat俟Q製 ileum contraction


Seyed Hassan Hejazian1, Seyyed Majid Bagheri2, Fatemeh Safari2 
1 Department of Physiology/Herbal Medicine Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
2 Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran

Correspondence Address:
Seyed Hassan Hejazian
Department of Physiology/Herbal Medicine Research Center, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd
Iran

Background: Trachyspermum ammi is a plant of umbelliferae family and has been traditionally used in the treatment of many gastrointestinal disorders such as indigestion, colic, and diarrhea. Our previous study demonstrated that aqueous extract of Trachyspermum ammi reduced the contractile activity of rat俟Q製 ileum. Aims: This study was designed to examine the effect of Trachyspermum ammi essence on acetylcholine-induced contraction in isolated rat俟Q製 ileum. Materials and Methods: In this experimental procedure, the tissue samples were suspended from the transducer lever and mounted on the organ bath containing Tyrode俟Q製 solution. Isotonic responses were recorded by using an isotonic transducer and the amplitude of contractions. This process was induced by cumulative logarithmic concentrations of acetylcholine before and after exposing tissue by saline and different concentrations of essence. Results: The chemical constituents of the essence from distillate extract of Trachyspermum ammi seeds were studied by gas chromatography mass spectrometry (GC-MS) and the chemical analysis showed many components in which thymol was the main constituent. Our findings showed that the essence derived from the extract in all concentrations used in this study (0.002, 0.005, and 0.01% V/V) significantly reduced acetylcholine-induced contractions (47.34, 60.46, and 86%, respectively, P < 0.05). The same concentrations of the essence also exhibit a significant anti-spasmodic action on acetylcholine-induced contractions (0.1,88.3, and 90.7%, respectively, P < 0.05). Conclusion: Since thymol was the major constituent of our samples of Trachyspermum ammi, the relaxant effect observed in this study is probably due to this agent which can exert an anti-cholinergic property.


How to cite this article:
Hejazian SH, Bagheri SM, Safari F. Spasmolytic and anti-spasmodic action of Trachyspermum ammi essence on rat's ileum contraction.North Am J Med Sci 2014;6:643-647


How to cite this URL:
Hejazian SH, Bagheri SM, Safari F. Spasmolytic and anti-spasmodic action of Trachyspermum ammi essence on rat's ileum contraction. North Am J Med Sci [serial online] 2014 [cited 2020 Sep 28 ];6:643-647
Available from: http://www.najms.org/article.asp?issn=1947-2714;year=2014;volume=6;issue=12;spage=643;epage=647;aulast=Hejazian;type=0