North American Journal of Medical Sciences

AUTHORS REPLY
Year
: 2014  |  Volume : 6  |  Issue : 3  |  Page : 160-

Western blot: Technique, theory and trouble shooting


Zhi-Qiang Liu, Tahrin Mahmood, Ping-Chang Yang 
 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON
Canada




How to cite this article:
Liu ZQ, Mahmood T, Yang PC. Western blot: Technique, theory and trouble shooting.North Am J Med Sci 2014;6:160-160


How to cite this URL:
Liu ZQ, Mahmood T, Yang PC. Western blot: Technique, theory and trouble shooting. North Am J Med Sci [serial online] 2014 [cited 2020 Jun 3 ];6:160-160
Available from: http://www.najms.org/text.asp?2014/6/3/160/128482


Full Text

Dear Editor,

We appreciate the comments [1] regarding our published article "Western blot: Technique, theory, and trouble-shooting". [2] For transferring of proteins to a membrane, the method of "wet transfer" in Western blotting is routinely utilized by laboratories and guided by the protocols online. [3] Heat may be produced during the transfer; according to the recommended protocols, placing the transfer apparatus on ice or placing it in the cold room is necessary. It should be considered that the purpose of 'Figure 7' in our article [2] is to show how to cool down the environmental temperature to 4°C by placing the apparatus on ice, which cannot be misunderstood as submerging the electroporator or the power leads in the water bath. In addition, we agree with the proposal that safe laboratory practice should always be observed to avoid chemical or electrical hazards in operating the Western blot transferring systems.

References

1Gibbons J. Western blot: Protein transfer overview. N Am J Med Sci 2014;6:158-9.
2Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. N Am J Med Sci 2012;4:429-34.
3Protocol Online. (Accessed December 25, 2013, at www.protocolonline.org/prot/Molecular_Biology/Protein/Western_Blotting ).