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  Indian J Med Microbiol
 

Figure 2: Identify the target vector by restriction analysis using the diagnostic restriction site. 1 negative control, 2 empty plasmid, 3 empty plasmid digested by other enzyme, 4 positive clone 1 digest by selected enzyme which yield 1.3 kb production, 5 positive clone 2, 6 positive clone 3, 7 positive clone 4, 7 positive clone 5, 8 positive clone 6, 9 Marker,

Figure 2: Identify the target vector by restriction analysis using the diagnostic restriction site. 1 negative control, 2 empty plasmid, 3 empty plasmid digested by other enzyme, 4 positive clone 1 digest by selected enzyme which yield 1.3 kb production, 5 positive clone 2, 6 positive clone 3, 7 positive clone 4, 7 positive clone 5, 8 positive clone 6, 9 Marker,