Figure 3: a. Positive staining to vessels using Alexa Fluor 594® conjugated anti-human IgG (red staining, white arrows). The yellowish staining is reactivity to FITC conjugated fibrinogen. In 3b. Positive staining to a long vessel using IIF on ME; however, in this case using FITC conjugated anti-human IgG (green staining, white arrows). The red staining represents the nuclei of adjacent cells, stained with Tropro3/DNA® In 3c. ME, with positive staining of the vessels on the periphery with ICAM-1/CD54 (red staining) (white arrow). The yellowish staining was seen with FITC conjugated anti-human albumin (blue arrow), also colocalizing with the vessels reactivity. In 3d. IHC staining against albumin; note some BMZ staining (blue arrow) and some intraepidermal staining between keratinocytes (red arrow). In 3e, IHC staining against IgM; note some intercellular staining between the keratinocytes (red arrows). In 3f, IHC displaying strong positivity of IgE in cells around the upper vessels of the skin (red arrows, brown staining). In 3g By IIF, ME positive round epithelial cells reactivity when using Alexa Fluor 597® anti-human IgE (green arrows). Of note, the staining pattern resembles the positivity of antibodies to filaggrin. The nuclei of the cells were counterstained with Dapi (blue). In 3h and 3i., again IIF on ME Some perinuclear and pericytoplasmic staining of the epithelial layer utilizing Alexa Fluor 597® conjugated anti-human IgG (red staining, white arrows). 3j, Similar to 3i, but at higher magnification. In 3k. Positive staining of ME epithelium intimal layer with FITC conjugated antihuman IgE (green staining). The red staining represents positivity of Alexa Fluor 597® conjugated anti-human IgG as intranuclear and pericytoplasmic staining in the epithelium of the esophagus (white arrows). In 3l. IIF on ME, displaying positive staining in an unusual pattern in the muscularis layer of the esophagus (green staining, white arrows).