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  Indian J Med Microbiol
 

Figure 1:a (10x) b (20x) H&E tissue sections demonstrates a subepidermal blistering and within the blister lumen, numerous lymphocytes, histiocytes, eosinophils and neutrophils are present (black arrows). c (10x) and d (40x). IHC positive myeloperoxidase positive inside the blister (red arrows). e . DIF showing positive stain against the superficial dermal vessels under the inflammatory process when using anti-human fibrinogen-FITCI conjugated (green stain, white arrows). The red stain is antibody to collagen IV corroborating that these are vessels. f . H&E shows reorganization of the vessels around the hair follicles (black arrows). g through i . IHC using CD34, showing how most of the vessels around the inflammatory process are reorganized and loss their normal distribution on the skin (red arrows). j . DIF, showing positive stain with anti-collagen VI antibody (yellowish stain, red arrow) at the base membrane zone (BMZ) of the skin as well as also show reorganization of the superficial and intermediate vessels around the inflammatory process (blue arrows). k . IHC using anti-body against collagen IV (dark stain) shows the stain on the sweat glands, and also show some reorganization of the vessels around the sweat glands with some kind of polarization towards the inflammation (red arrows). 1 DIF, showing positive stain with anti-human IgM-FITCI conjugated to the sweat glands (yellow stain (white arrow). The nuclei were counterstained with Dapi (blue) and the sweat glands with mapped with anti-collagen VI antibody (red stain).

Figure 1:a (10x) b (20x) H&E tissue sections demonstrates a subepidermal blistering and within the blister lumen, numerous lymphocytes, histiocytes, eosinophils and neutrophils are present (black arrows). c (10x) and d (40x). IHC positive myeloperoxidase positive inside the blister (red arrows). e . DIF showing positive stain against the superficial dermal vessels under the inflammatory process when using anti-human fibrinogen-FITCI conjugated (green stain, white arrows). The red stain is antibody to collagen IV corroborating that these are vessels. f . H&E shows reorganization of the vessels around the hair follicles (black arrows). g through i . IHC using CD34, showing how most of the vessels around the inflammatory process are reorganized and loss their normal distribution on the skin (red arrows). j . DIF, showing positive stain with anti-collagen VI antibody (yellowish stain, red arrow) at the base membrane zone (BMZ) of the skin as well as also show reorganization of the superficial and intermediate vessels around the inflammatory process (blue arrows). k . IHC using anti-body against collagen IV (dark stain) shows the stain on the sweat glands, and also show some reorganization of the vessels around the sweat glands with some kind of polarization towards the inflammation (red arrows). 1 DIF, showing positive stain with anti-human IgM-FITCI conjugated to the sweat glands (yellow stain (white arrow). The nuclei were counterstained with Dapi (blue) and the sweat glands with mapped with anti-collagen VI antibody (red stain).