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  Indian J Med Microbiol
 

Figure 1: a and c. H&E staining at lower and higher magnification demonstrates a mild to moderate perivascular and perinfundibular infiltrate, with loss of sebaceous glands (black arrow). b . Positive IHC staining with anti-human p53 against the germinal area of the hair follicle (dark staining; red arrow). d. Overexpressed HLA-ABC by IHC in hair follicle areas (dark brown staining; red arrow). The HLA-ABC marker was also detected at a normal level in the epidermis and dermal blood vessels. e. Overexpressed MAC (Complement/C5b-9) by IHC inside the hair follicles and also around them (dark brown staining; red and black arrows). f. Positive DIF staining with rhodamine conjugated anti-human IgM inside the isthmus of the hair follicle (red staining; white arrow). The nuclei of the cells were counterstained with DAPI (blue-white staining). g. Strongly positive DIF staining for Complement/C3 inside the hair follicle (yellow/green staining; red arrow). h. Positive DIF staining for FITC conjugated Complement/C3 inside the hair follicle (green staining; black arrow). The nuclei of the keratinocytes were counterstained with 4', 6-diamidino-2-phenylindole (DAPI; blue-white staining). Please note on the left side of the hair follicle a large group of damaged cells, highlighted by the DAPI staining (red arrow). i. Strongly positive DIF staining with fibrinogen around the hair follicle (green staining; red arrows). j. A clinically alopecic area in the patient's hair (white arrow). k. Positive IHC staining with anti-human survivin (dark brown staining; red arrow). 1. Positive IHC staining in some parts of the hair follicle with bcl-2 ( dark brown staining; red arrows).

Figure 1: a and c. H&E staining at lower and higher magnification demonstrates a mild to moderate perivascular and perinfundibular infiltrate, with loss of sebaceous glands (black arrow). b . Positive IHC staining with anti-human p53 against the germinal area of the hair follicle (dark staining; red arrow). d. Overexpressed HLA-ABC by IHC in hair follicle areas (dark brown staining; red arrow). The HLA-ABC marker was also detected at a normal level in the epidermis and dermal blood vessels. e. Overexpressed MAC (Complement/C5b-9) by IHC inside the hair follicles and also around them (dark brown staining; red and black arrows). f. Positive DIF staining with rhodamine conjugated anti-human IgM inside the isthmus of the hair follicle (red staining; white arrow). The nuclei of the cells were counterstained with DAPI (blue-white staining). g. Strongly positive DIF staining for Complement/C3 inside the hair follicle (yellow/green staining; red arrow). h. Positive DIF staining for FITC conjugated Complement/C3 inside the hair follicle (green staining; black arrow). The nuclei of the keratinocytes were counterstained with 4', 6-diamidino-2-phenylindole (DAPI; blue-white staining). Please note on the left side of the hair follicle a large group of damaged cells, highlighted by the DAPI staining (red arrow). i. Strongly positive DIF staining with fibrinogen around the hair follicle (green staining; red arrows). j. A clinically alopecic area in the patient's hair (white arrow). k. Positive IHC staining with anti-human survivin (dark brown staining; red arrow). 1. Positive IHC staining in some parts of the hair follicle with bcl-2 ( dark brown staining; red arrows).