Figure 3: DCs can capture the nasal epithelial cell-derived exosomes. DCs were generated from isolated PBMC and cultured in RPMI1640 medium. Avb6, or Derp1, or SEB/Derp1-carrying exosomes were added to the culture. The cells were collected 6 h later and analyzed by flow cytometry and immunocytochemistry respectively. A1-B1, the flow cytometry dot plots show SEB+ or Derp1+ DCs. A2-B2, the representative confocal images show SEB+ staining (in green) and Derp1+ cells (in red). The yellow color was merged by green and red colors. The data represent 3 experiments.