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 Table of Contents  
TECHNICAL ARTICLE
Year : 2012  |  Volume : 4  |  Issue : 9  |  Page : 429-434

Western blot: Technique, theory, and trouble shooting

Tahrin Mahmood, Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Date of Web Publication14-Sep-2012

Correspondence Address:
Ping-Chang Yang
Department of Pathology & Molecular Medicine, McMaster University, Room T3303, 50 Charlton Ave East, Hamilton, ON
Canada
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.100998

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  Abstract 

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.

Keywords: Bio-medical research, protein, western blot


How to cite this article:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. North Am J Med Sci 2012;4:429-34

How to cite this URL:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. North Am J Med Sci [serial online] 2012 [cited 2022 Aug 13];4:429-34. Available from: https://www.najms.org/text.asp?2012/4/9/429/100998


  Introduction Top


Western blot is often used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight, and thus by type, through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labels antibodies specific to the protein of interest.

The unbound antibody is washed off leaving only the bound antibody to the protein of interest. The bound antibodies are then detected by developing the film. As the antibodies only bind to the protein of interest, only one band should be visible. The thickness of the band corresponds to the amount of protein present; thus doing a standard can indicate the amount of protein present. The paper will first describe the protocol for western blot, accompanied by pictures to help the reader and theory to rationalize the protocol. This will be followed by the theoretical explanation of the procedure, and in the later section, troubleshooting tips for common problems.

Technique

Cell lysis to extract protein

Protein can be extracted from different kind of samples, such as tissue or cells. Below is the protocol to extract proteins from adherent cells.

Adherent cells:

  1. Wash cells in the tissue culture flask or dish by adding cold phosphate buffered saline (PBS) and rocking gently. Discard PBS. (Tip: Keep tissue culture dish on ice throughout).
  2. Add PBS and use a cell scraper to dislodge the cells. Pipette the mixture into microcentrifuge tubes.
  3. Centrifuge at 1500 RPM for 5 minutes and discard the supernatant.
  4. Add 180 μL of ice cold cell lysis buffer with 20 μL fresh protease inhibitor cocktail. (Tip: If protein concentration is not high enough at the end, it is advised to repeat the procedure with a higher proportion of protease inhibitor cocktail).
  5. Incubate for 30 minutes on ice, and then clarify the lysate by spinning for 10 minutes at 12,000 RPM, at 4°C.
  6. Transfer supernatant (or protein mix) to a fresh tube and store on ice or frozen at -20°C or -80°C.
  7. Measure the concentration of protein using a spectrophotometer.
Sample preparation



  1. determine the volume of protein extract to ensure 50 μg in each well.
  2. Add 5 μL sample buffer to the sample, and make the volume in each lane equalized using double distilled H 2 O (dd H 2 O). Mix well. (Tip: Total volume of 15 μL per lane is suggested).
  3. Heat the samples with dry plate for 5 minutes at 100°C.


Gel preparation



  1. After preparing the 10% stacking gel solution, assemble the rack for gel solidification [Figure 1]. (Tip: 10% AP and TEMED solidify the solution; therefore, both gels can be prepared at the same time, if the abovementioned reagents are not added until the end).
  2. Add stacking gel solution carefully until the level is equal to the green bar holding the glass plates [Figure 2]. Add H 2 O to the top. Wait for 15-30 minutes until the gel turning solidified. (Tip: Using a suction pipette can make the process of adding the gel to the glass plate easier).
  3. Overlay the stacking gel with the separating gel, after removing the water. (Tip: It is better to tilt the apparatus and use a paper towel to remove the water).
  4. Insert the comb, ensuring that there are no air bubbles.
  5. Wait until the gel is solidified. (Tip: Solidification can be easily checked by leaving some gel solution in a tube).
Figure 1: Assembled rack for gel solidification

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Figure 2: Add gel solution using a transfer pipette

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Electrophoresis

  1. Pour the running buffer into the electrophorator [Figure 3].
  2. Place gel inside the electrophorator and connect to a power supply. (Tip: When connecting to the power source always connect red to red, and black to black).
  3. Make sure buffer covers the gel completely, and remove the comb carefully.
  4. Load marker (6 μL) followed by samples (15 μL) in to each well [Figure 4].
  5. Run the gel with low voltage (60 V) for separating gel; use higher voltage (140 V) for stacking gel [Figure 5]a and b.
  6. Run the gel for approximately an hour, or until the dye front runs off the bottom of the gel [Figure 6].
Figure 3: Add running buffer to the electrophorator

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Figure 4: Add samples and molecular marker to the gel, after removing the combs

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Figure 5: (a) Samples running through the stacking gel (lower voltage). (b): Samples running through the separating gel (higher voltage)

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Figure 6: Run the gel to the bottom of the electrophorator

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Electrotransfer

  1. Cut 6 filter sheets to fit the measurement of the gel, and one polyvinylidene fluoride (PDVF) membrane with the same dimensions.
  2. Wet the sponge and filter paper in transfer buffer, and wet the PDVF membrane in methanol.
  3. Separate glass plates and retrieve the gel.
  4. Create a transfer sandwich as follows:

    Sponge

    3 Filter Papers

    Gel PVDF

    3 Filter Papers

    (Tip: Ensure there are no air bubbles between the gel and PVDF membrane, and squeeze out extra liquid).
  5. Relocate the sandwich to the transfer apparatus, which should be placed on ice to maintain 4°C. Add transfer buffer to the apparatus, and ensure that the sandwich is covered with the buffer. Place electrodes on top of the sandwich, ensuring that the PVDF membrane is between the gel and a positive electrode [Figure 7].
  6. Transfer for 90 minutes [Figure 8]. (Tip: The running time should be proportional to the thickness of the gel, so this may be reduced to 45 minutes for 0.75 mm gels).
Figure 7: Transfer should be done on ice

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Figure 8: Membrane after transfer

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Blocking and antibody incubation

  1. Block the membrane with 5% skim milk in TBST* for 1 hour.
  2. Add primary antibody in 5% bovine serum albumin ( BSA) and incubate overnight in 4°C on a shaker [Figure 9].
  3. Wash the membrane with TBST for 5 minutes. Do this 3 times. (Tip: All washing and antibody incubation steps should be done on a shaker at room temperature to ensure even agitation).
  4. Add secondary antibody in 5% skim milk in TBST, and incubate for 1 hour.
  5. Wash the membrane with TBST for 5 minutes. Do this 3 times
  6. Prepare ECL mix (following the proportion of solution A and B provided by the manufacturer). Incubate the membrane for 1-2 minutes [Figure 10]. (Tip: Use a 1000 μL pipette to ensure that ECL covers the top and bottom of the membrane).
  7. Visualize the result in the dark room [Figure 11]. (Tip: If the background is too strong, reduce exposure time).
*, TBST: Tris-Buffered Saline Tween-20
Figure 9: Use a shaker to incubate the membrane with antibody

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Figure 10: Incubate the membrane with ECL mix using a 1000 μL pipette to help the process

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Figure 11: Use the cassette to expose the membrane in the dark room

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Recipe

  1. Dissolve the following in 800 ml of distilled H 2 O

    • 8.8 g of NaCl
    • 0.2g of KCl
    • 3g of Tris base
  2. Add 500ul of Tween-20
  3. Adjust the pH to 7.4
  4. Add distilled H 2 O to 1L
  5. Sterilize by filtration or autoclaving
Theory

Sample preparation

Cell lysates are the most common form of sample used for western blot. Protein extraction attempts to collect all the proteins in the cell cytosol. This should be done in a cold temperature with protease inhibitors to prevent denaturing of the proteins. Since tissue sample display a higher degree of structure, mechanical invention, such as homogenization, or sonication is needed to extract the proteins.

After extracting the protein, it is very important to have a good idea of the extract's concentration. This eventually allows the researcher to ensure that the samples are being compared on an equivalent basis. Protein concentration is often measured using a spectrophotometer. Using this concentration allows to measure the mass of the protein that is being loaded into each well by the relationship between concentration, mass, and volume.

After determining the appropriate volume of the sample, it is diluted into a loading buffer, which contains glycerol so that the samples sink easily into the wells of the gel. A tracking dye (bromophenol blue) is also present in the buffer allowing the researcher to see how far the separation has progressed. The sample is heated after being diluted into a loading buffer, in order to denature the higher order structure, while retaining sulfide bridges. Denaturing the high structure ensures that the negative charge of amino acids is not neutralized, enabling the protein to move in an electric field (applied during electrotransfer).

It is also very important to have positive and negative controls for the sample. For a positive control a known source of target protein, such as purified protein or a control lysate is used. This helps to confirm the identity of the protein, and the activity of the antibody. A negative control is a null cell line, such as β-actin, is used as well to confirm that the staining is not nonspecific.

Gel electrophoresis

Western blot uses two different types of agarose gel: stacking and separating gel. The higher, stacking gel is slightly acidic (pH 6.8) and has a lower acrylamide concentration making a porous gel, which separates protein poorly but allows them to form thin, sharply defined bands. The lower gel, called the separating, or resolving gel, is basic (pH 8.8), and has a higher polyacrylamide content, making the gel's pores narrower. Protein is thus separated by their size more so in this gel, as the smaller proteins to travel more easily, and hence rapidly, than larger proteins.

The proteins when loaded on the gel have a negative charge, as they have been denatured by heating, and will travel toward the positive electrode when a voltage is applied. Gels are usually made by pouring them between two glass or plastic plates, using the solution described in the protocol section. The samples and a marker are loaded into the wells, and the empty wells are loaded with sample buffer. The gel is then connected to the power supply and allowed to run. The voltage is very important, as a high voltage can overheat and distort the bands.

Blotting

After separating the protein mixture, it is transferred to a membrane. The transfer is done using an electric field oriented perpendicular to the surface of the gel, causing proteins to move out of the gel and onto the membrane. The membrane is placed between the gel surface and the positive electrode in a sandwich. The sandwich includes a fiber pad (sponge) at each end, and filter papers to protect the gel and blotting membrane [Figure 12]. Here two things are very important: (1) the close contact of gel and membrane to ensure a clear image and (2) the placement of the membrane between the gel and the positive electrode. The membrane must be placed as such, so that the negatively charged proteins can migrate from the gel to the membrane. This type of transfer is called electrophoretic transfer, and can be done in semi-dry or wet conditions. Wet conditions are usually more reliable as it is less likely to dry out the gel, and is preferred for larger proteins.
Figure 12: Assembly of a sandwich in western Blot

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The membrane, the solid support, is an essential part of this process. There are two types of membrane: nitrocellulose and PVDF. Nitrocellulose is used for its high affinity for protein and its retention abilities. However, it is brittle, and does not allow the membrane to be used for reprobing. In this regard, PVDF membranes provide better mechanical support and allow the blot to be reprobed and stored. However, the background is higher in the PVDF membranes and therefore, washing carefully is very important.

Washing, blocking and antibody incubation

Blocking is a very important step of western blotting, as it prevents antibodies from binding to the membrane nonspecifically. Blocking is often made with 5% BSA or nonfat dried milk diluted in TBST to reduce the background.

Nonfat dried milk is often preferred as it is inexpensive and widely available. However, milk proteins are not compatible with all detection labels, so care must be taken to choose the appropriate blocking solution. For example, BSA blocking solutions are preferred with biotin and AP antibody labels, and antiphosphoprotein antibodies, since milk contains casein, which is itself a phosphoprotein and biotin, thus interfering with the assay results. It is often a good strategy to incubate the primary antibody with BSA since it is usually needed in higher amounts than the secondary antibody. Putting it in BSA solution allows the antibody to be reused, if the blot does not give good result.

The concentration of the antibody depends on the instruction by the manufacturer. The antibody can be diluted in a wash buffer, such as PBS or TBST. Washing is very important as it minimized background and removes unbound antibody. However, the membrane should not be left to wash for a really long time, as it can also reduce the signal.

The membrane is then detected using the label antibody, usually with an enzyme such as horseradish peroxidase (HRP), which is detected by the signal it produces corresponding to the position of the target protein. This signal is captured on a film which is usually developed in a dark room.

Quantification

It is very important to be aware that the data produced with a western blot is typically considered to be semi-quantitative. This is because it provides a relative comparison of protein levels, but not an absolute measure of quantity. There are two reasons for this; first, there are variations in loading and transfer rates between the samples in separate lanes which are different on separate blots. These differences will need to be standardized before a more precise comparison can be made. Second, the signal generated by detection is not linear across the concentration range of samples. Thus, since the signal produced is not linear, it should not be used to model the concentration.

Troubleshooting

Even though the procedure for western blot is simple, many problems can arise, leading to unexpected results. The problem can be grouped into five categories: (1) unusual or unexpected bands, (2) no bands, (3) faint bands or weak signal, (4) high background on the blot, and (5) patchy or uneven spots on the blot.

Unusual or unexpected bands can be due to protease degradation, which produces bands at unexpected positions. In this case it is advisable to use a fresh sample which had been kept on ice or alter the antibody. If the protein seems to be in too high of a position, then reheating the sample can help to break the quaternary protein structure. Similarly, blurry bands are often caused by high voltage or air bubbles present during transfer. In this case, it should be ensured that the gel is run at a lower voltage, and that the transfer sandwich is prepared properly. In addition, changing the running buffer can also help the problem. Nonflat bands can be the result of too fast of a travel through the gel, due to low resistance. To fix this the gel should be optimized to fit the sample. Finally, white (negative) bands on the film are due to too much protein or antibody.

Another problem: no bands can also arise due to many reasons related to antibody, antigen, or buffer used. If an improper antibody is used, either primary or secondary, the band will not show. In addition, the concentration of the antibody should be appropriate as well; if the concentration is too low, the signal may not be visible. It is important to remember that some antibodies are not to be used for western blot. Another reason for no visible bands is the lowest concentration or absence of the antigen. In this case, antigen from another source can be used to confirm whether the problem lies with the sample or with other elements, such as the antibody. Moreover, prolonged washing can also decrease the signal. Buffers can also contribute to the problem. It should be ensured that buffers like the transfer buffer, TBST, running buffer and ECL are all new and noncontaminated. If the buffers are contaminated with sodium azide, it can inactivate HRP.

Similarly, weak signals can be caused by low concentration of antibody or antigen. Increasing exposure time can also help to make the band clearer. Another reason could be nonfat dry milk masking the antigen. In this case use BSA or decrease the amount of milk used.

High background is often caused by too high concentration of the antibody, which can bind to PVDF membranes. Another problem could be the buffers, which may be too old. Increasing the washing time can also help to decrease the background. Additionally, too high of an exposure can also lead to this problem. Therefore, it is advisable to check different exposure times to achieve an optimum time.

Patchy and uneven spots on the blot are usually caused by improper transfer. If there are air bubbles trapped between the gel and the membrane, it will appear darker on the film. It is also important to use a shaker for all incubation, so that there is no uneven agitation during the incubation. Once again, washing is of utmost importance as well to wash the background. This problem can also be caused by antibodies binding to the blocking agents; in this case another blocking agent should be tried. Filtering the blocking agent can also help to remove some contaminants. Finally, this problem can also be caused by aggregation of the secondary antibody; in this case, the secondary antibody should be centrifuged and filtered to remove the aggregated.


  Conclusion Top


Western blot is a technique that is very useful for protein detection as it allows the user to quantify the protein expression as well. This paper covered the protocol, the theory behind that protocol, and some troubleshooting techniques. Western blot can be seen as an intricate balance, as the researcher attempts to get a nonspecific, yet strong signal.


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6], [Figure 7], [Figure 8], [Figure 9], [Figure 10], [Figure 11], [Figure 12]


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38 Amino acid restriction induces a long non-coding RNA UBA6-AS1 to regulate GCN2-mediated integrated stress response in breast cancer
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40 Glycyrrhiza uralensis root extract ameliorates high glucose-induced renal proximal tubular fibrosis by attenuating tubular epithelial-myofibroblast transdifferentiation by targeting TGF-ß1/Smad/Stat3 pathway
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41 Transcription Dependent Loss of an Ectopically Expressed Variant Surface Glycoprotein during Antigenic Variation in Trypanosoma brucei
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42 ChGn-2 Plays a Cardioprotective Role in Heart Failure Caused by Acute Pressure Overload
Andreas Haryono, Koji Ikeda, Dhite Bayu Nugroho, Takehiro Ogata, Yumika Tsuji, Satoaki Matoba, Kensuke Moriwaki, Hiroshi Kitagawa, Michihiro Igarashi, Ken-ichi Hirata, Noriaki Emoto
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43 Coronas of micro/nano plastics: a key determinant in their risk assessments
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44 Runt-Related Transcription Factor 2 (Runx2) Measurement in Phytoestrogen-Induced Bone: A Comparison of Western Blot and Immunohistochemistry Methods
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45 Western blotting: a powerful staple in scientific and biomedical research
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46 Emergence of High Prevalence of Extended-Spectrum Beta-Lactamase and Carbapenemase Producing Acinetobacter Species and Pseudomonas aeruginosa Among Hospitalized Patients at Dessie Comprehensive Specialized Hospital, North-East Ethiopia
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47 Aptamer Validation by Western Blot–an overview
Lamees Jamal Talib , Basma Talib Al-Sudani , Mustafa Ghazi Al-Abbassi
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48 LncAABR07025387.1 Enhances Myocardial Ischemia/Reperfusion Injury Via miR-205/ACSL4-Mediated Ferroptosis
Weixin Sun, Xiang Wu, Peng Yu, Qian Zhang, Le Shen, Jiandong Chen, Huaqin Tong, Manlu Fan, Haibo Shi, Xiaohu Chen
Frontiers in Cell and Developmental Biology. 2022; 10
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49 ICAM-1 on Breast Cancer Cells Suppresses Lung Metastasis but Is Dispensable for Tumor Growth and Killing by Cytotoxic T Cells
Ofer Regev, Marina Kizner, Francesco Roncato, Maya Dadiani, Massimo Saini, Francesc Castro-Giner, Olga Yajuk, Stav Kozlovski, Nehora Levi, Yoseph Addadi, Ofra Golani, Shifra Ben-Dor, Zvi Granot, Nicola Aceto, Ronen Alon
Frontiers in Immunology. 2022; 13
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50 Leveraging Bulk and Single-Cell RNA Sequencing Data of NSCLC Tumor Microenvironment and Therapeutic Potential of NLOC-15A, A Novel Multi-Target Small Molecule
Bashir Lawal, Alexander T. H. Wu, Hsu-Shan Huang
Frontiers in Immunology. 2022; 13
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51 Pharmacological Responses of the G542X-CFTR to CFTR Modulators
Xinxiu Fang, Jiunn-Tyng Yeh, Tzyh-Chang Hwang
Frontiers in Molecular Biosciences. 2022; 9
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52 Combined Systemic Intake of K-ATP Opener (Nicorandil) and Mesenchymal Stem Cells Preconditioned With Nicorandil Alleviates Pancreatic Insufficiency in a Model of Bilateral Renal Ischemia/Reperfusion Injury
Asmaa Mohammed ShamsEldeen, Sarah A. Abd El-Aal, Basma Emad Aboulhoda, Hend AbdAllah, Sara Mahmoud Gamal, Fatma E. Hassan, Marwa Nagi Mehesen, Laila Ahmed Rashed, Abeer Mostafa, Nermeen Bakr Sadek
Frontiers in Physiology. 2022; 13
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53 Consolidating and Upscaling Molecular Research Capacity in Nigeria: On Who's Account?
Chinwe Uzoma Chukwudi
Frontiers in Research Metrics and Analytics. 2022; 6
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54 Identification and Characterization of P0 Protein as a Vaccine Candidate Against Babesia divergens, Blood Parasite of Veterinary and Zoonotic Importance
Shimaa Abd El-Salam El-Sayed, Mohamed Abdo Rizk, Haitham Eldoumani, Shimaa Sobhy Sorour, Mohamad Alaa Terkawi, Mahmoud AbouLaila, Ikuo Igarashi, Mohamed Z. Sayed-Ahmed
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55 An In-Depth Study on the Metabolite Profile and Biological Properties of Primula auriculata Extracts: A Fascinating Sparkle on the Way from Nature to Functional Applications
Inci Kurt-Celep, Dimitrina Zheleva-Dimitrova, Reneta Gevrenova, Abdullahi Ibrahim Uba, Gokhan Zengin, Evren Yildiztugay, Carene Marie Nancy Picot-Allain, José Manuel Lorenzo, Mohamad Fawzi Mahomoodally, Domenico Montesano
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56 Chemistry and Analysis of Organic Compounds in Dinosaurs
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57 Nano-Resveratrol: A Promising Candidate for the Treatment of Renal Toxicity Induced by Doxorubicin in Rats Through Modulation of Beclin-1 and mTOR
Ahlam M. Alhusaini, Laila M. Fadda, Abeer M. Alanazi, Wedad S. Sarawi, Hatun A. Alomar, Hanaa M. Ali, Iman H. Hasan, Rehab Ahmed Ali
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58 Simultaneous Visualization of MiRNA-221 and Caspase-3 in Cancer Cells for Investigating the Feasibility of MiRNA-Targeted Therapy with a Dual-Color Fluorescent Nanosensor
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59 Development and Validation of Rapid In-House Diagnostic ELISA Kits for Detection of Human Orthopneumovirus in Clinical Samples
Ibrahim M. Aziz, Mohamed A. Farrag, Rauf Bhat, Anwar Ahmed, Noorah A. Alkubaisi, Rasha M. Alzayed, Gani Asa Dudin, Fahad N. Almajhdi
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60 In Vitro Tumor Cell-Binding Assay to Select High-Binding Antibody and Predict Therapy Response for Personalized 64Cu-Intraperitoneal Radioimmunotherapy against Peritoneal Dissemination of Pancreatic Cancer: A Feasibility Study
Fukiko Hihara, Hiroki Matsumoto, Mitsuyoshi Yoshimoto, Takashi Masuko, Yuichi Endo, Chika Igarashi, Tomoko Tachibana, Mitsuhiro Shinada, Ming-Rong Zhang, Gene Kurosawa, Aya Sugyo, Atsushi B. Tsuji, Tatsuya Higashi, Hiroaki Kurihara, Makoto Ueno, Yukie Yoshii
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61 Picrasidine I Triggers Heme Oxygenase-1-Induced Apoptosis in Nasopharyngeal Carcinoma Cells via ERK and Akt Signaling Pathways
Hsin-Yu Ho, Ping-Ju Chen, Yi-Ching Chuang, Yu-Sheng Lo, Chia-Chieh Lin, Ming-Ju Hsieh, Mu-Kuan Chen
International Journal of Molecular Sciences. 2022; 23(11): 6103
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62 Analyzing BMP2, FGFR, and TGF Beta Expressions in High-Grade Osteosarcoma Untreated and Treated Autografts Using Proteomic Analysis
Rashmi Madda, Chao-Ming Chen, Cheng-Fong Chen, Jir-You Wang, Hsin-Yi Wu, Po-Kuei Wu, Wei-Ming Chen
International Journal of Molecular Sciences. 2022; 23(13): 7409
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63 Ameliorating Effects of TRIM67 against Intestinal Inflammation and Barrier Dysfunction Induced by High Fat Diet in Obese Mice
Qihui Luo, Asad Jahangir, Junbo He, Chao Huang, Yu Xia, Lanlan Jia, Xiaoli Wei, Ting Pan, Yanni Du, Bin Mu, Huan Gong, Wentao Liu, Saif Ur-Rehman, Kangcheng Pan, Zhengli Chen
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64 Evaluation of the Performance of a ZnO-Nanoparticle-Coated Hydrocolloid Patch in Wound Healing
Van Anh Thi Le, Tung X. Trinh, Pham Ngoc Chien, Nguyen Ngan Giang, Xin-Rui Zhang, Sun-Young Nam, Chan-Yeong Heo
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65 Antineoplastic Activity of Water-Soluble Form of Novel Kinase Inhibitor 1-(4-Chlorobenzyl)-3-Chloro-4-(3-Trifluoromethylphenylamino)-1H-Pyrrole-2,5-Dione Immobilized on Polymeric Poly (PEGMA-co-DMM) Carrier
Nataliya Finiuk, Olga Klyuchivska, Nataliya Mitina, Halyna Kuznietsova, Kateryna Volianiuk, Alexander Zaichenko, Volodymyr Rybalchenko, Rostyslav Stoika
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66 Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus
Michaela Mrkvová, Richard Hancinský, Simona Grešíková, Šarlota Kanuková, Ján Barilla, Miroslav Glasa, Pavol Hauptvogel, Ján Kraic, Daniel Mihálik
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67 Heat Treatment Improves UV Photoprotective Effects of Licorice in Human Dermal Fibroblasts
Jeong-Yong Park,Yun-Jeong Ji,Kyung Hye Seo,Ji Yeon Lee,Geum-Soog Kim,Min Hye Kang,Jeong Hoon Lee,Gwi Yeong Jang,Hyung Don Kim
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68 Electrical Detection of Innate Immune Cells
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69 The Level of Mesenchymal-Epithelial Transition Autophosphorylation is Correlated with Esophageal Squamous Cell Carcinoma Migration
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70 Sweet Basil Has Distinct Synthases for Eugenol Biosynthesis in Glandular Trichomes and Roots with Different Regulatory Mechanisms
Vaishnavi Amarr Reddy,Chunhong Li,Kumar Nadimuthu,Jessica Gambino Tjhang,In-Cheol Jang,Sarojam Rajani
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71 Enhanced Production of the Mical Redox Domain for Enzymology and F-actin Disassembly Assays
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72 Proteomics of Homeobox7 Enhanced Salt Tolerance in Mesembryanthemum crystallinum
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73 Novel NF?B Inhibitor SC75741 Mitigates Chondrocyte Degradation and Prevents Activated Fibroblast Transformation by Modulating miR-21/GDF-5/SOX5 Signaling
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74 Trimethylamine n-Oxide (TMAO) Modulates the Expression of Cardiovascular Disease-Related microRNAs and Their Targets
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75 Influence of Risk Factors for Male Infertility on Sperm Protein Composition
Marie Bisconti, Jean-François Simon, Sarah Grassi, Baptiste Leroy, Baptiste Martinet, Vanessa Arcolia, Vladimir Isachenko, Elise Hennebert
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76 Design, Synthesis, In Vitro Anticancer Evaluation and Molecular Modelling Studies of 3,4,5-Trimethoxyphenyl-Based Derivatives as Dual EGFR/HDAC Hybrid Inhibitors
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77 Scorpion Venom-Functionalized Quercetin Phytosomes for Breast Cancer Management: In Vitro Response Surface Optimization and Anticancer Activity against MCF-7 Cells
Nabil A. Alhakamy, Usama A. Fahmy, Shaimaa M. Badr Eldin, Osama A. A. Ahmed, Hibah M. Aldawsari, Solomon Z. Okbazghi, Mohamed A. Alfaleh, Wesam H. Abdulaal, Abdulmohsin J. Alamoudi, Fatma M. Mady
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78 From Donor to the Lab: A Fascinating Journey of Primary Cell Lines
Magdalena Richter,Oliwia Piwocka,Marika Musielak,Igor Piotrowski,Wiktoria M. Suchorska,Tomasz Trzeciak
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79 Wastewater-Based Epidemiology as an Early Warning System for the Spreading of SARS-CoV-2 and Its Mutations in the Population
Tomáš Mackulak,Miroslav Gál,Viera Špalková,Miroslav Fehér,Katarína Briestenská,Miriam Mikušová,Karolína Tomcíková,Michal Tamáš,Andrea Butor Škulcová
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80 Chrysosplenol D Triggers Apoptosis through Heme Oxygenase-1 and Mitogen-Activated Protein Kinase Signaling in Oral Squamous Cell Carcinoma
Ming-Ju Hsieh,Chia-Chieh Lin,Yu-Sheng Lo,Yi-Ching Chuang,Hsin-Yu Ho,Mu-Kuan Chen
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81 Beyond the Warburg Effect: Oxidative and Glycolytic Phenotypes Coexist within the Metabolic Heterogeneity of Glioblastoma
Tomás Duraj,Noemí García-Romero,Josefa Carrión-Navarro,Rodrigo Madurga,Ana Ortiz de Mendivil,Ricardo Prat-Acin,Lina Garcia-Cańamaque,Angel Ayuso-Sacido
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82 Challenges with Methods for Detecting and Studying the Transcription Factor Nuclear Factor Kappa B (NF-?B) in the Central Nervous System
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83 A Preclinical Investigation of GBM-N019 as a Potential Inhibitor of Glioblastoma via Exosomal mTOR/CDK6/STAT3 Signaling
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84 Amelioration of Diabetic Nephropathy by Targeting Autophagy via Rapamycin or Fasting: Relation to Cell Apoptosis/Survival
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85 Prediction and Experimental Verification of a Hierarchical Transcription Factor Regulatory Network of Porcine Myoglobin (Mb)
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86 Imperatorin Interferes with LPS Binding to the TLR4 Co-Receptor and Activates the Nrf2 Antioxidative Pathway in RAW264.7 Murine Macrophage Cells
Mei-Hsuen Huang,Yu-Hsien Lin,Ping-Chiang Lyu,Yi-Chung Liu,Yuan-Shiun Chang,Jing-Gung Chung,Wei-Yong Lin,Wen-Tsong Hsieh
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87 Unraveling the Local Relation Between Tissue Composition and Human Brain Mechanics Through Machine Learning
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88 Effect of Berberine on Hyperuricemia and Kidney Injury: A Network Pharmacology Analysis and Experimental Validation in a Mouse Model
Qiaoping Li,Ziwei Huang,Defu Liu,Jingna Zheng,Jianhui Xie,Jiannan Chen,Huifang Zeng,Ziren Su,Yucui Li
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89 Transmission mode of watermelon silver mottle virus by Thrips palmi
De-Fen Mou,Wei-Te Chen,Wei-Hua Li,Tsung-Chi Chen,Chien-Hao Tseng,Li-Hsin Huang,Jui-Chu Peng,Shyi-Dong Yeh,Chi-Wei Tsai,Livia Maria Silva Ataide
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90 Identification of a diguanylate cyclase expressed in the presence of plants and its application for discovering candidate gene products involved in plant colonization by Pantoea sp. YR343
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91 An Assessment of In-vitro and In-vivo Evaluation Methods for Theranostic Nanomaterials
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92 Cell volume homeostatically controls the rDNA repeat copy number and rRNA synthesis rate in yeast
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94 The study of interaction of different forms of human recombinant anti-mullerian hormone with a chimeric analogue of the AMH type II
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95 Involvement of the VEGF signaling pathway in immunosuppression and hypoxia stress: analysis of mRNA expression in lymphocytes mediating panting in Jersey cattle under heat stress
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96 Integrative analysis of genome-wide DNA methylation and single-nucleotide polymorphism identified ACSM5 as a suppressor of lumbar ligamentum flavum hypertrophy
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97 Activation of PLCß1 enhances endocannabinoid mobilization to restore hippocampal spike-timing-dependent potentiation and contextual fear memory impaired by Alzheimer’s amyloidosis
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98 Umbilical mesenchymal stem cell-derived extracellular vesicles as enzyme delivery vehicle to treat Morquio A fibroblasts
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99 Increased susceptibility of cystic fibrosis airway epithelial cells to ferroptosis
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100 Nano-Curcumin Protects Against Sodium Nitrite–Induced Lung Hypoxia Through Modulation of Mitogen-Activated Protein Kinases/c-Jun NH2-Terminal Kinase Signaling Pathway
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101 Antioxidative and Skin Protective Effects of Canarium subulatum Methanol Extract on Keratinocytes
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102 Metabolic responsiveness to training depends on insulin sensitivity and protein content of exosomes in insulin-resistant males
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103 A multi-epitope chimeric protein elicited a strong antibody response and partial protection against Edwardsiella ictaluri in Nile tilapia
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104 Interaction Study of Different Forms of Human Recombinant Anti-Mullerian Hormone with a Chimeric Analogue of the AMH Type II Receptor
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105 Endoplasmic reticulum-unfolded protein response signalling is altered in severe eosinophilic and neutrophilic asthma
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106 Icaritin alleviates docetaxel-induced skin injury by suppressing reactive oxygen species via estrogen receptors
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107 Overexpression of vacuolar H + -pyrophosphatase (H + -PPase) gene from Ammopiptanthus nanus enhances drought tolerance in maize
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108 PVT1 Knockdown Inhibits Autophagy and Improves Gemcitabine Sensitivity by Regulating the MiR-143/HIF-1a/VMP1 Axis in Pancreatic Cancer
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110 The role of glycosylation in the N-terminus of the hemagglutinin of a unique H4N2 with a natural polybasic cleavage site in virus fitness in vitro and in vivo
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111 Human circular RNA hsa_circRNA_101705 (circTXNDC11) regulates renal cancer progression by regulating MAPK/ERK pathway
Chong-Yi Yang,Jie Wang,Jian-Qiu Zhang,Hong-miao Cai
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112 Studies in Lung cancer cytokine proteomics: A review
Hanieh Abolfathi,Mojgan Sheikhpour,Seyed Sadegh Shahrayini,Shohreh Khatami,Seyed Ali Nojoumi
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113 A vaccine for photodynamic immunogenic cell death: tumor cell caged by cellular disulfide–thiol exchange for immunotherapy
Ya Wen,Yiqiong Liu,Fangfang Guo,Yi Han,Qiansai Qiu,Yan Li,Haiqing Dong,Tianbin Ren,Yongyong Li
Biomaterials Science. 2021;
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114 A freeze-and-thaw-induced fragment of the microtubule-associated protein tau in rat brain extracts: implications for the biochemical assessment of neurotoxicity
Israel C. Vasconcelos,Raquel M. Campos,Hanna K. Schwaemmle,Ana P. Masson,Gustavo D. Ferrari,Luciane C. Alberici,Vitor M. Faça,Norberto Garcia-Cairasco,Adriano Sebollela
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115 On-chip label-free impedance-based detection of antibiotic permeation
Jaspreet Kaur,Hamed Ghorbanpoor,Yasin Öztürk,Özge Kaygusuz,Hüseyin Avci,Cihan Darcan,Levent Trabzon,Fatma D. Güzel
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116 How Protein Labeling Applying
Bian Xinyu,Fan Sun,Zhixuan Xu,J. Sun,Z. Li,X. Liu
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117 Efficacy assessment of a triple anthrax chimeric antigen as a vaccine candidate in guinea pigs: challenge test with Bacillus anthracis 17 JB strain spores
Masoud Abdous,Sadegh Hasannia,Ali Hatef Salmanian,Seyed-Shahryar Arab
Immunopharmacology and Immunotoxicology. 2021; : 1
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118 Wheel running leads to sex-specific effects on Western diet-associated glucose homeostasis and brain insulin signaling without altering food-related impulsive choice
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Nutritional Neuroscience. 2021; : 1
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119 Characterization of Homogeneous and Heterogeneous Amyloid-ß42 Oligomer Preparations with Biochemical Methods and Infrared Spectroscopy Reveals a Correlation between Infrared Spectrum and Oligomer Size
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ACS Chemical Neuroscience. 2021;
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120 A Synchronized Increase of Stilbenes and Flavonoids in Metabolically Engineered Vitis vinifera cv. Gamay Red Cell Culture
Ru Wang,Sangram Keshari Lenka,Varun Kumar,Noga Sikron-Persi,Irena Dynkin,David Weiss,Avichai Perl,Aaron Fait,Michal Oren-Shamir
Journal of Agricultural and Food Chemistry. 2021;
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121 Comparing Protein Adsorption onto Alumina and Silica Nanomaterial Surfaces: Clues for Vaccine Adjuvant Development
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122 Development of a recombinant anti-VEGFR2-EPCAM bispecific antibody to improve antiangiogenic efficiency
Khadijeh Barzaman,Mitra Samadi,Shima Moradi-kalbolandi,Keivan Majidzadeh-A,Malihe Salehi,Neda Jalili,Mir hadi Jazayeri,Samaneh Khorammi,Behrad Darvishi,Vahid Siavashi,Mahdi Shekarabi,Leila Farahmand
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123 Linear Cryogel Arrays: On the Fast Track for Borreliosis Detection
Patrick L. Fosso Tene,Anne Stumpf,Marc Zinggeler,Volker Reuck,Arif Malik,Wilfried Weigel,Marina Müller,Richard Kneusel,Thomas Brandstetter,Jürgen Rühe
Analytical Chemistry. 2021;
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124 Functional proteomics protocol for the identification of interaction partners in Tetrahymena thermophila
Syed Nabeel-Shah,Jyoti Garg,Pata-Eting Kougnassoukou Tchara,Ronald E. Pearlman,Jean-Philippe Lambert,Jeffrey Fillingham
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125 Lentiviral-mediated ectopic expression of YAP and TAZ in YAPoff cancer cell lines
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126 The Fold-Illuminator: A low-cost, portable, and disposable incubator-illuminator device
Logan R. Burrington,Emran Baryal,Katherine Hui,Emmett Lambert,Sarah T. Harding,Javin P. Oza
Synthetic and Systems Biotechnology. 2021; 6(2): 95
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127 Antimycin A-induced mitochondrial dysfunction is consistent with impaired insulin signalling in cultured skeletal muscle cells
Sithandiwe E. Mazibuko-Mbeje,Sinenhlanhla X.H. Mthembu,Phiwayinkosi V. Dludla,Evelyn Madoroba,Nireshni Chellan,Abidemi P. Kappo,Christo J.F. Muller
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128 Mesoporous silica nanoparticles-based formulations of a chimeric proteinous vaccine candidate against necrotic enteritis disease
Zakieh Sadat Hoseini,Abbas Hajizade,Jamshid Razmyar,Gholamreza Ahmadian,Ayyoob Arpanaei
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129 Effect of suvorexant on morphine tolerance and dependence in mice: Role of NMDA, AMPA, ERK and CREB proteins
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NeuroToxicology. 2021; 84: 64
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130 Method for efficient soluble expression and purification of recombinant human interleukin-15
Nadeem Ahmed,Bakht Afroze,Rabia Abbas,Mohsin Ahmed Khan,Muhammad Akram,Saad Tahir,Shehman Bakht,Ayesha Munir,Ahmad Ali Shahid
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131 Refolding, purification, and characterization of constitutive-active human-Smad8 produced as inclusion bodies in ClearColi® BL21 (DE3)
Carla Lizbeth Segovia-Trinidad,Bastian Quaas,Zhaopeng Li,Antonina Lavrentieva,Yvonne Roger,Thomas Scheper,Andrea Hoffmann,Ursula Rinas
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132 A highly efficient, thermo stable and broad pH adaptable copper-zinc super oxide dismutase (AmSOD1) mediates hydrogen peroxide tolerance in Avicennia marina
Monireh Fesharaki-Esfahani,Azar Shahpiri,Akram Kazemi-Nasab
Phytochemistry. 2021; 187: 112766
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133 Lactobacillus paracasei PS23 dietary supplementation alleviates muscle aging via ghrelin stimulation in d-galactose-induced aging mice
Li-Hao Cheng,Shih-Hsuan Cheng,Chien-Chen Wu,Chin-Lin Huang,Pei-Jun Wen,Min-Yu Chang,Ying-Chieh Tsai
Journal of Functional Foods. 2021; 85: 104651
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134 Antidiabetic effects and mechanisms of action of ?-conglutin from lupin seeds
Mrunmai Tapadia,Stuart Johnson,Ranjeet Utikar,Philip Newsholme,Rodrigo Carlessi
Journal of Functional Foods. 2021; 87: 104786
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135 Gene expression of microbial gelatinase activity for porcine gelatine identification
Safiyyah Shahimi,Mohd. Fadly Lamri,Sahilah Abd. Mutalib,Rozida Mohd Khalid,Mahzan Md. Tab,Farahayu Khairuddin
Food Chemistry. 2021; : 129586
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136 Bacterial membrane vesicle functions, laboratory methods, and applications
PinarAytar Çelik, Burak Derkus, Kübra Erdogan, Dilan Barut, Enuh Blaise Manga, Yalin Yildirim, Simon Pecha, Ahmet Çabuk
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137 Synthesis of novel indole-thiazolidinone hybrid structures as promising scaffold with anticancer potential
Anna Kryshchyshyn-Dylevych,Lidia Radko,Nataliya Finiuk,Myroslav Garazd,Nataliya Kashchak,Andrzej Posyniak,Krzysztof Niemczuk,Rostyslav Stoika,Roman Lesyk
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138 Identification and biochemical characterisation of tyrosine aminotransferase from Anthoceros agrestis unveils the conceivable entry point into rosmarinic acid biosynthesis in hornworts
Tobias Busch,Maike Petersen
Planta. 2021; 253(5)
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139 Activation of Galanin Receptor 1 with M617 Attenuates Neuronal Apoptosis via ERK/GSK-3ß/TIP60 Pathway After Subarachnoid Hemorrhage in Rats
Hui Shi,Yuanjian Fang,Lei Huang,Ling Gao,Cameron Lenahan,Takeshi Okada,Zachary D. Travis,Shucai Xie,Hong Tang,Qin Lu,Rui Liu,Jiping Tang,Yuan Cheng,John H. Zhang
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140 The influence of P-glycoprotein expression in the standard treatment of Helicobacter pylori infection in Sprague Dawley rats
Noor Safwah Damanhuri,Endang Kumolosasi,Marhanis Salihah Omar,Amirul Faiz Abd Razak,Ahmad Hasnan Mansor
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141 Effects of perampanel on cognitive behavior and GluR1 expression in immature mice of temporal lobe epilepsy
Ting Wang, Lin wang, Limin Li, Le Ma, Xiaohong Liu
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142 Development of methods for detecting the fate of mesenchymal stem cells regulated by bone bioactive materials
Jiang Le,Liu Zhongqun,Wang Zhaoyan,Su Yijun,Wang Yingjin,Wei Yaojie,Jiang Yanan,Jia Zhanrong,Ma Chunyang,Gang Fangli,Xu Nan,Zhao Lingyun,Wang Xiumei,Wu Qiong,Lu Xiong,Sun Xiaodan
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143 Serological assays and host antibody detection in coronavirus-related disease diagnosis
Sayeh Dowlatshahi,Ehsan Shabani,Mohammad J. Abdekhodaie
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144 Expression of Genes and Proteins of the Sarcoplasmic Reticulum ??2+-Transport Systems in Cardiomyocytes in Concomitant Coronary Heart Disease and Type 2 Diabetes Mellitus
S. A. Afanas’ev, D. S. Kondrat’eva, E. F. Muslimova, ?. V. Budnikova, Sh. D. Akhmedov, B. N. Kozlov
Bulletin of Experimental Biology and Medicine. 2021; 172(2): 117
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145 Rapid fabrication of teflon apertures by controlled high voltage pulses for formation of free standing planar lipid bilayer membrane
Yasin Ozturk,Aliakbar Ebrahimi,Araz Norouz Dizaji,Ozge Kaygusuz,Jayesh Arun Bafna,Mathias Winterhalter,Guven Cankaya,Cihan Darcan,Fatma Dogan Guzel
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146 THz Detection of Biomolecules in Aqueous Environments—Status and Perspectives for Analysis Under Physiological Conditions and Clinical use
Christian Weisenstein,Anna Katharina Wigger,Merle Richter,Robert Sczech,Anja Katrin Bosserhoff,Peter Haring Bolívar
Journal of Infrared, Millimeter, and Terahertz Waves. 2021;
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147 A guide to mass spectrometric analysis of extracellular vesicle proteins for biomarker discovery
Iqbal Jalaludin, David M. Lubman, Jeongkwon Kim
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148 Association of Type 1 diabetes with ulcerative colitis in BALB/c mice: Investigations on sex-specific differences
Shivani Singla, Chittaranjan Sahu, Gopabandhu Jena
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149 Long noncoding RNA TUG1 upregulates VEGFA to enhance malignant behaviors in stomach adenocarcinoma by sponging miR-29c-3p
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150 Nanophase surface arrays on poly (lactic-co-glycolic acid) upregulate neural cell functions
Didem Mimiroglu,Tulin Yanik,Batur Ercan
Journal of Biomedical Materials Research Part A. 2021;
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151 Mn 2+ -Phos-Tag Polyacrylamide for the Quantification of Protein Phosphorylation Levels
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152 A novel method for purification of biologically active C -terminal fragment of human recombinant anti-mullerian hormone
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Biomedical Chromatography. 2021;
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153 Trehalose protects the endothelium from cadmium-induced dysfunction
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Cell Biology International. 2021;
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154 Neuroprotective efficacy of nano-CoQ against propionic acid toxicity in rats: Role of BDNF and CREB protein expressions
Ahlam Alhusaini,Iman H. Hasan,Bashayer Alrumayyan,Marwa Alesikri,Khansa Alanazi,Rawan Almasoud,Sarah Almarshad
Journal of Biochemical and Molecular Toxicology. 2020;
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155 Identification and characterization of a novel isoform of heparin cofactor II in human liver
Shadabi Bano,Sana Fatima,Shahzaib Ahamad,Shoyab Ansari,Dinesh Gupta,Mohammad Tabish,Sayeed ur Rehman,Mohamad Aman Jairajpuri
IUBMB Life. 2020;
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156 Questioning coverage values determined by 2D western blots: A critical study on the characterization of anti-HCP ELISA reagents
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Biotechnology and Bioengineering. 2020;
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157 Purification of human recombinant anti-mullerian hormone and its derivatives
Alexandra Ya. Rak,Alexander V. Trofimov,Natalia V. Pigareva,Eugeny A. Protasov,Elena A. Karabanova,Alexander M. Ischenko
Biomedical Chromatography. 2020;
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158 Triclosan induces zebrafish neurotoxicity by abnormal expression of miR-219 targeting oligodendrocyte differentiation of central nervous system
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159 Combinatorial ethanol treatment increases the overall productivity of recombinant hG-CSF in E. coli: a comparative study
Balaram Mishra,Giridharan Murthy,Bijayalaxmi Sahoo,Sang Jun Uhm,Mukesh Kumar Gupta
Applied Microbiology and Biotechnology. 2020;
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160 Phenolic metabolism in the hornwort Anthoceros agrestis: 4-coumarate CoA ligase and 4-hydroxybenzoate CoA ligase
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161 Identifying adaptive alleles in the human genome: from selection mapping to functional validation
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Human Genetics. 2020;
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162 Halodule pinifolia (Seagrass) attenuated lipopolysaccharide-, carrageenan-, and crystal-induced secretion of pro-inflammatory cytokines: mechanism and chemistry
Sajeli Ahil Begum,Kirti Hira,Pragya Paramita Pal,Samrun Nessa,Onkar P. Kulkarni,Jeyapragash Danaraj,Ameer Basha Shaik,Hiroshi Araya,Yoshinori Fujimoto
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163 Pachymic acid protects against cerebral ischemia/reperfusion injury by the PI3K/Akt signaling pathway
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164 (-)-Epicatechin reduces muscle waste after complete spinal cord transection in a murine model: role of ubiquitin–proteasome system
Cristian Gonzalez-Ruiz,Paola Cordero-Anguiano,Axayacatl Morales-Guadarrama,Rodrigo Mondragón-Lozano,Stephanie Sánchez-Torres,Hermelinda Salgado-Ceballos,Francisco Villarreal,Eduardo Meaney,Guillermo Ceballos,Nayelli Nájera
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165 Antibody stability: A key to performance - Analysis, influences and improvement
Hui Ma,Ciarán Ó’Fágáin,Richard O’Kennedy
Biochimie. 2020; 177: 213
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166 Scaffolds with controlled release of pro-mineralization exosomes to promote craniofacial bone healing without cell transplantation
W. Benton Swanson,Zhen Zhang,Kemao Xiu,Ting Gong,Miranda Eberle,Ziqi Wang,Peter X. Ma
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167 Impact of liraglutide on microcirculation in experimental diabetic cardiomyopathy
Nehad Gamal Abdelrazik Soliman,Ahmed A.M. Abdel-Hamid,Amany A. El-Hawwary,Amany Ellakkany
Acta Histochemica. 2020; 122(3): 151533
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168 Low METTL3 level in midgut of the Bombyx mori inhibit the proliferation of nucleopolyhedrovirus
Peng Xue,Tao Jiang,Juan Zhu,Meixian Wang,Qiaoling Zhao,Jinshan Huang,Shunming Tang,Xingjia Shen
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169 Improvement of immunodetection of the transcription factor C/EBP homologous protein by western blot
Jonathan Turpin,Etienne Frumence,Daed El Safadi,Olivier Meilhac,Pascale Krejbich-Trotot,Wildriss Viranaďcken
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170 Molecular characterization of Antheraea mylitta arylphorin gene and its encoded protein
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Archives of Biochemistry and Biophysics. 2020; 692: 108540
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171 Toona sinensis modulates autophagy and cytokines in lipopolysaccharide-induced RAW 264.7 macrophages
Chun-En Aurea Kuo,Szu-Ying Wu,Chen-Hsiang Lee,Yun-Ru Lai,Cheng-Hsien Lu,Po-Cheng Chen,Jai-Hong Cheng,Li-Yu Tsai,Ke-Tien Yen,Yu Tsao,Shih-Meng Tsai
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172 Low dose HSP90 inhibition with AUY922 blunts rapid evolution of metastatic and drug resistant phenotypes induced by TGF-ß and paclitaxel in A549 cells
Nickolas A. Bacon,Isabel Larre,Abdalla A. Lawag,Carlen Merritt,Mackinzie Smith,Matthew Rosolen,Vincent E. Sollars
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173 The functional differentiation of four smad4 paralogs in TGF-ß signaling pathway of Japanese flounder (Paralichthys olivaceus)
Haiyang Yu,Yujue Wang,Chaofan Jin,Yuxiang Liu,Yan He,Quanqi Zhang
Cellular Signalling. 2020; 71: 109601
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174 Effect of mobile phone signal radiation on epigenetic modulation in the hippocampus of Wistar rat
Ranjeet Kumar,Pravin S. Deshmukh,Sonal Sharma,Basu Dev Banerjee
Environmental Research. 2020; : 110297
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175 Ligand- and structural-based discovery of potential small molecules that target the colchicine site of tubulin for cancer treatment
Qingqing Guo,Huimin Zhang,Yanhong Deng,Shiyang Zhai,Zhenla Jiang,Daqian Zhu,Ling Wang
European Journal of Medicinal Chemistry. 2020; 196: 112328
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176 Delayed effects of sulfur mustard on autophagy suppression in chemically-injured lung tissue
Marzieh Eghtedardoost,Tooba Ghazanfari,Alireza Sadeghipour,Zuhair Mohammad Hassan,Mostafa Ghanei,Saeid Ghavami
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177 Production of pharmaceutical active recombinant globular adiponectin as a secretory protein in Withania Somnifera hairy root culture
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178 TCR-like domain antibody against Mycobacterium tuberculosis (Mtb) heat shock protein antigen presented by HLA-A*11 and HLA-A*24
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179 Identification and sub-cellular localization of a NAD transporter in Leishmania braziliensis (LbNDT1)
David S. Morales Herrera,Luis E. Contreras Rodríguez,Claudia C. Rubiano Castellanos,Maria H. Ramírez Hernández
Heliyon. 2020; 6(7): e04331
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180 PXR is a target of (-)-epicatechin in skeletal muscle
Miguel Ortiz-Flores,Andrés Portilla-Martínez,Francisco Cabrera-Pérez,Nayelli Nájera,Eduardo Meaney,Francisco Villarreal,Javier Pérez-Durán,Guillermo Ceballos
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181 Impact of in ovo administered pioneer colonizers on intestinal proteome on day of hatch
K.M. Wilson,D.R. Rodrigues,W.N. Briggs,A.F. Duff,K.M. Chasser,W.G. Bottje,L.R. Bielke
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182 The Mediation of Pigeon Egg Production by Regulating the Steroid Hormone Biosynthesis of Pigeon Ovarian Granulosa Cells
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183 Bioinspired 3D porous human placental derived extracellular matrix/silk fibroin sponges for accelerated bone regeneration
Arun Prabhu Rameshbabu,Kamakshi Bankoti,Sayanti Datta,Elavarasan Subramani,Anupam Apoorva,Paulomi Ghosh,Subhodeep Jana,Padmavati Manchikanti,Sabyasachi Roy,Koel Chaudhury,Santanu Dhara
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184 Cloning and expression of OdTx12, a ß excitatory toxin from Odontobuthus doriae, in Escherichia coli and evaluation of its bioactivity in Locusta migratoria
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185 An amplification strategy for detecting HER2 with a quasi-targeted proteomics approach coupled with aptamer-triggered hybridization chain reaction
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186 Review: Detection and quantification of proteins in human urine
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187 Harvesting, processing, and evaluation of in vitro-manipulated equine preantral follicles: A review
E.L. Gastal,F.L.N. Aguiar,G.D.A. Gastal,K.A. Alves,B.G. Alves,J.R. Figueiredo
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188 A capillary electrophoresis based approach for the identification of anti-drug antibodies against camelid VHH biologics (Nanobodies®)
Derek Wiswell,Divas Neupane,Minchao Chen,Edward P. Bowman,Douglas Linn,Anandi Sawant,Alissa Chackerian,Shuli Zhang,Enrique Escandón
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189 Soluble epoxide hydrolase inhibitors improve angiogenic function of endothelial progenitor cells via ERK/p38-mediated miR-126 upregulation in myocardial infarction mice after exercise
Yajun Gui,Jingyuan Chen,Jiahui Hu,Caixiu Liao,Minzhi Ouyang,Limin Deng,Jingmin Yang,Danyan Xu
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190 Antibody validation for Western blot: By the user, for the user
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Journal of Biological Chemistry. 2020; 295(4): 926
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191 Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients
Francisco G. Ortega,Matías D. Regiart,Alba Rodríguez-Martínez,Diego de Miguel-Pérez,María J. Serrano,José A. Lorente,Gonzalo Tortella,Olga Rubilar,Karim Sapag,Mauro Bertotti,Martín A. Fernández-Baldo
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192 Liver Phosphoenolpyruvate Carboxykinase-1 Downregulation via siRNA-Functionalized Graphene Oxide Nanosheets Restores Glucose Homeostasis in a Type 2 Diabetes Mellitus In Vivo Model
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193 Inhibiting Matrix Metalloproteinase-2 Activation by Perturbing Protein–Protein Interactions Using a Cyclic Peptide
Priyanka Sarkar,Zhonghan Li,Wendan Ren,Siwen Wang,Shiqun Shao,Jianan Sun,Xiaodong Ren,Nicole G. Perkins,Zhili Guo,Chia-En A. Chang,Jikui Song,Min Xue
Journal of Medicinal Chemistry. 2020;
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194 Unraveling How Ethanol-Induced Conformational Changes Affect BSA Protein Adsorption onto Silica Surfaces
Jia Ying Brenda Tan,Bo Kyeong Yoon,Gamaliel Junren Ma,Tun Naw Sut,Nam-Joon Cho,Joshua A. Jackman
Langmuir. 2020;
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195 A Review of Properties, Delivery Systems and Analytical Methods for the Characterization of Monomeric Glycoprotein Transferrin
Mariza Aires Fernandes,Gilmar Hanck-Silva,Franciele Garcia Baveloni,Joăo Augusto Oshiro Junior,Felipe Tita de Lima,Josimar O. Eloy,Marlus Chorilli
Critical Reviews in Analytical Chemistry. 2020; : 1
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196 Perillyl alcohol suppresses monocrotaline-induced pulmonary arterial hypertension in rats via anti-remodeling, anti-oxidant, and anti-inflammatory effects
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Clinical and Experimental Hypertension. 2020; : 1
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197 Acceleration of somatic cell reprogramming into the induced pluripotent stem cell using a mycosporine-like amino acid, Porphyra 334
Junsang Yoo,Junyeop Kim,Jeong Hun Lee,Hyein Kim,Sung Joo Jang,Hyo Hyun Seo,Seung Taek Oh,Seung Jae Hyeon,Hoon Ryu,Jongpil Kim,Sang Hyun Moh
Scientific Reports. 2020; 10(1)
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198 Antibody validation for Western blot: By the user, for the user
Lakshmi Pillai-Kastoori,Sam Heaton,Steve D. Shiflett,Annabelle C. Roberts,Alejandra Solache,Amy R. Schutz-Geschwender
Journal of Biological Chemistry. 2020; 295(4): 926
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199 Synthesis of Palladium(II) Complexes via Michael Addition: Antiproliferative Effects through ROS-Mediated Mitochondrial Apoptosis and Docking with SARS-CoV-2
Jebiti Haribabu,Swaminathan Srividya,Dharmasivam Mahendiran,Dasararaju Gayathri,Vemula Venkatramu,Nattamai Bhuvanesh,Ramasamy Karvembu
Inorganic Chemistry. 2020;
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200 Assessing the Immune Response When Raising Antibodies for Use in Xenopus
Maya Z. Piccinni, Matthew J. Guille
Cold Spring Harbor Protocols. 2020; 2020(9): pdb.prot10
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201 Exosomal miRNAs as circulating biomarkers for prediction of development of haematogenous metastasis after surgery for stage II/III gastric cancer
Xin Liu,Kent-Man Chu
Journal of Cellular and Molecular Medicine. 2020;
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202 Extracts of Moringa oleifera leaves from different cultivation regions show both antioxidant and antiobesity activities
Da-Song Kim,Moon-Hee Choi,Hyun-Jae Shin
Journal of Food Biochemistry. 2020;
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203 Deciphering key regulators involved in epilepsy-induced cardiac damage through whole transcriptome and proteome analysis in a rat model
Supriya Sharma,Meetal Sharma,Anil Kumar Rana,Robin Joshi,Mohit Kumar Swarnkar,Vishal Acharya,Damanpreet Singh
Epilepsia. 2020;
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204 Influence of salinity on survival, growth, hemolymph osmolality, gill sodium potassium ATPase activity, and sodium potassium chloride co-transporter expression in the redclaw crayfish Cherax quadricarinatus
Reem Rida,Razan Zein-Eddine,Sawsan Kreydiyyeh,Antonio Garza de Yta,Imad Patrick Saoud
Journal of the World Aquaculture Society. 2020;
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205 Comparative Molecular Biology Approaches for the Production of Poliovirus Virus-Like Particles Using Pichia pastoris
Lee Sherry,Keith Grehan,Joseph S. Snowden,Michael L. Knight,Oluwapelumi O. Adeyemi,David J. Rowlands,Nicola J. Stonehouse,Urs F. Greber
mSphere. 2020; 5(2)
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206 Erigeron annuus Protects PC12 Neuronal Cells from Oxidative Stress Induced by ROS-Mediated Apoptosis
Ji Yeon Lee,Jeong-Yong Park,Dong Hwi Kim,Hyung Don Kim,Yun-Jeong Ji,Kyung Hye Seo
Evidence-Based Complementary and Alternative Medicine. 2020; 2020: 1
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207 Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in ß-Amyloid-Induced Rats Model of Alzheimer’s Disease
Theng Choon Ooi,Munirah Ahmad Munawar,Nur Hasnieza Mohd Rosli,Siti Nur Aqilah Abdul Malek,Hanisah Rosli,Farah Wahida Ibrahim,Norazrina Azmi,Hasnah Haron,Razinah Sharif,Suzana Shahar,Nor Fadilah Rajab
Evidence-Based Complementary and Alternative Medicine. 2020; 2020: 1
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208 Preparation of polyclonal and monoclonal anti-idiotypic antibodies against morphine-specific immunoglobulins
A. V. Trofimov,V. A. Atanesyan,A. M. Ischenko,E. A. Karabanova,A. Ya. Rak,A. S. Simbirtsev,N. B. Gamaleya,A. G. Berzina,N. V. Stankova,G. D. Kapanadze,L. I. Ulyanova,T. A. Klimova
Medical Immunology (Russia). 2020; 22(1): 187
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209 Cyanidin 3-O-Glucoside Induces the Apoptosis in the Osteosarcoma Cells through Upregulation of the PPAR? and P21: An In Vitro Study
Hesam A. Atashi,Hamid Z. Arani,Amirhossein Shekarriz,Hamidreza Nazari,Amirhossein Zabolian,Rasul Rakhshan,Maedeh Olya
Anti-Cancer Agents in Medicinal Chemistry. 2020; 20(9): 1087
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210 A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)
ANTONIA EFSTATHIOU,Despina Smirlis
BIO-PROTOCOL. 2020; 10(2)
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211 Inhibitor of protein kinases 1-(4-chlorobenzyl)-3-chloro-4-(3-trifluoromethylphenylamino)-1?-pyrrole-2,5-dione induces DNA damage and apoptosis in human colon carcinoma cells
N. S. Finiuk,O. Yu. Klyuchivska,H. M. Kuznietsova,S. P. Vashchuk,V. K. Rybalchenko,R. S. Stoika
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212

Upregulation of FTX Promotes Osteosarcoma Tumorigenesis by Increasing SOX4 Expression via miR-214-5p
Haicong Chen,Tianfeng Liu,Hanbin Ouyang,Sien Lin,Huan Zhong,Hongwu Zhang,Yang Yang
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213 Identifying Antioxidant Proteins by Using Amino Acid Composition and Protein-Protein Interactions
Yixiao Zhai,Yu Chen,Zhixia Teng,Yuming Zhao
Frontiers in Cell and Developmental Biology. 2020; 8
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214 A Single Step in vitro Bioassay Mimicking TLR4-LPS Pathway and the Role of MD2 and CD14 Coreceptors
Pramod Jagtap,Puja Prasad,Abhishek Pateria,Sachin D. Deshmukh,Shalini Gupta
Frontiers in Immunology. 2020; 11
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215 Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
Martin Kello,Peter Takac,Peter Kubatka,Tomas Kuruc,Klaudia Petrova,Jan Mojzis
Biomolecules. 2020; 10(1): 139
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216 Measurement of the Emission Spectra of Protein Solutions in the Infrared Range. Description of the Method and Testing Using Solution of Human Interferon Gamma as an Example
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Frontiers in Physics. 2020; 8
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217 Azasteroid Alkylators as Dual Inhibitors of AKT and ERK Signaling for the Treatment of Ovarian Carcinoma
Panagiotis Dalezis,Eleni Geromichalou,Aikaterini Polonifi,Sofia Sagredou,Nikolaos Nikoleousakos,Michael Nikolaou,Vasiliki Sarli,Mihalis I. Panayiotidis,Dimitrios T. Trafalis
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218 Insertion of Basic Amino Acids in the Hemagglutinin Cleavage Site of H4N2 Avian Influenza Virus (AIV)—Reduced Virus Fitness in Chickens is Restored by Reassortment with Highly Pathogenic H5N1 AIV
Marcel Gischke,Reiner Ulrich,Olanrewaju I. Fatola,David Scheibner,Ahmed H. Salaheldin,Beate Crossley,Eva Böttcher-Friebertshäuser,Jutta Veits,Thomas C. Mettenleiter,Elsayed M. Abdelwhab
International Journal of Molecular Sciences. 2020; 21(7): 2353
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219 A Higher Frequency Administration of the Nontoxic Cycloartane-Type Triterpene Argentatin A Improved Its Anti-Tumor Activity
Zaira Tavarez-Santamaría,Nadia J. Jacobo-Herrera,Leticia Rocha-Zavaleta,Alejandro Zentella-Dehesa,Beatriz del Carmen Couder-García,Mariano Martínez-Vázquez
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220 Development of Epidermal Equivalent from Electrospun Synthetic Polymers for In Vitro Irritation/Corrosion Testing
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221 Samnamul (Shoots of Aruncus dioicus) Inhibit Adipogenesis by Downregulating Adipocyte-Specific Transcription Factors in 3T3-L1 Adipocytes
Ji Yeon Lee,Jeong-Yong Park,Hyun Taek Seo,Hyun-A Seong,Yun-Jeong Ji,Seung Eun Lee,Kyung Hye Seo,Hyung Don Kim
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222 Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways
Sithandiwe Mazibuko-Mbeje,Phiwayinkosi Dludla,Candice Roux,Rabia Johnson,Samira Ghoor,Elizabeth Joubert,Johan Louw,Andy Opoku,Christo Muller
International Journal of Molecular Sciences. 2019; 20(3): 633
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223 Gypenoside LXXV Promotes Cutaneous Wound Healing In Vivo by Enhancing Connective Tissue Growth Factor Levels Via the Glucocorticoid Receptor Pathway
Sungjoo Park,Eunsu Ko,Jun Hyoung Lee,Yoseb Song,Chang-Hao Cui,Jingang Hou,Byeong Min Jeon,Hun Sik Kim,Sun Chang Kim
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224 Complementarity of Matrix- and Nanostructure-Assisted Laser Desorption/Ionization Approaches
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Nanomaterials. 2019; 9(2): 260
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225 Preclinical Evidence of STAT3 Inhibitor Pacritinib Overcoming Temozolomide Resistance via Downregulating miR-21-Enriched Exosomes from M2 Glioblastoma-Associated Macrophages
Hao-Yu Chuang,Yu-kai Su,Heng-Wei Liu,Chao-Hsuan Chen,Shao-Chih Chiu,Der-Yang Cho,Shinn-Zong Lin,Yueh-Sheng Chen,Chien-Min Lin
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226 Primary Membranous Glomerulonephritis: The Role of Serum and Urine Biomarkers in Patient Management
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227 Knockdown of TIGAR Induces Apoptosis and Autophagy with Modulates NF-?B and HO-1 Expression in A549 Lung Cancer Cells
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228 Western Blotting and Flow Cytometry for Evaluating Necroptosis of Cells (Review)
A. S. Tkachenko,A. I. Onishchenko,V. G. Gopkalov,T. V. Gorbach,E. A. Kharchenko,D. O. Sklyaruk
Ukraďns'kij žurnal medicini, běologěď ta sportu. 2019; 4(4): 32
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229 Biomarkers of Multiple Sclerosis
Daina Pastare,Mohamed Ridha Bennour,Elina Polunosika,Guntis Karelis
The Open Immunology Journal. 2019; 9(1): 1
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230 Diagnostic potential of saliva proteome analysis: a review and guide to clinical practice
Camilla Vieira Esteves,Wladimir Gushiken de Campos,Milena Monteiro de Souza,Silvia Vanessa Lourenço,Walter Luiz Siqueira,Celso Augusto Lemos-Júnior
Brazilian Oral Research. 2019; 33(0)
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231 Comparative analysis of homologous aminopeptidase PepN from pathogenic and non-pathogenic mycobacteria reveals divergent traits
Nishant Sharma,Suruchi Aggarwal,Saravanan Kumar,Rahul Sharma,Konika Choudhury,Niti Singh,Praapti Jayaswal,Renu Goel,Saima Wajid,Amit Kumar Yadav,Krishnamohan Atmakuri,Yung-Fu Chang
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232 Aspalathin, a natural product with the potential to reverse hepatic insulin resistance by improving energy metabolism and mitochondrial respiration
Sithandiwe E. Mazibuko-Mbeje,Phiwayinkosi V. Dludla,Rabia Johnson,Elizabeth Joubert,Johan Louw,Khanyisani Ziqubu,Luca Tiano,Sonia Silvestri,Patrick Orlando,Andy R. Opoku,Christo J. F. Muller,Michael Bader
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233 The role of NF-?B and Elk-1 in the regulation of mouse ADAM17 expression
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Biology Open. 2019; 8(2): bio039420
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234 Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway
Chen-chen Cai,Jiang-hu Zhu,Li-xia Ye,Yuan-yuan Dai,Ming-chu Fang,Ying-ying Hu,Shu-lin Pan,Si Chen,Pei-jun Li,Xiao-qin Fu,Zhen-lang Lin
Oxidative Medicine and Cellular Longevity. 2019; 2019: 1
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235 A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts
Yang Zheng,Fankang Meng,Zihui Zhu,Weijia Wei,Zhi Sun,Jinchun Chen,Bo Yu,Chunbo Lou,Guo-Qiang Chen
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236 A new triple chimeric protein as a high immunogenic antigen against anthrax toxins: theoretical and experimental analyses
Masoud Abdous,Sadegh Hasannia,Ali Hatef Salmanian,Seyed Shahryar Arab,Abbas Shali,Ghorban Ali Alizadeh,Afshin Hajizadeh,Abolfazl Khafri,Ammar Mohseni
Immunopharmacology and Immunotoxicology. 2019; : 1
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237 EspA-loaded mesoporous silica nanoparticles can efficiently protect animal model against enterohaemorrhagic E. coli O157: H7
Abbas Hajizade,Ali Hatef Salmanian,Jafar Amani,Firouz Ebrahimi,Ayyoob Arpanaei
Artificial Cells, Nanomedicine, and Biotechnology. 2019; : 1
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238 Functional neutralization of anti-IFN-? autoantibody in patients with nontuberculous mycobacteria infection
Dyah Ika Krisnawati,Yung-Ching Liu,Yuarn-Jang Lee,Yun-Ting Wang,Chia-Ling Chen,Po-Chun Tseng,Chiou-Feng Lin
Scientific Reports. 2019; 9(1)
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239 Quantification of ERK Kinase Activity in Biological Samples Using Differential Sensing
Diana Zamora-Olivares,Tamer S. Kaoud,Lingyu Zeng,Jacey R. Pridgen,Deborah L. Zhuang,Yakndara E. Ekpo,Jessica R. Nye,Mitchell Telles,Eric V. Anslyn,Kevin N. Dalby
ACS Chemical Biology. 2019;
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240 Analyzing bacterial extracellular vesicles in human body fluids by orthogonal biophysical separation and biochemical characterization
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241 Platelet Factor 4 as a Novel Exosome Marker in MALDI-MS Analysis of Exosomes from Human Serum
Huu-Quang Nguyen,Dabin Lee,Yeoseon Kim,Minseok Paek,Minsun Kim,Kyoung-Soon Jang,Jooyeon Oh,Young-Sun Lee,Jong Eun Yeon,David M. Lubman,Jeongkwon Kim
Analytical Chemistry. 2019;
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242 miR-133b suppresses colorectal cancer cell stemness and chemoresistance by targeting methyltransferase DOT1L
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243 Targeted quantitative proteomic analysis of drug metabolizing enzymes and transporters by nano LC-MS/MS in the sandwich cultured human hepatocyte model
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244 AVE 0991 attenuates oxidative stress and neuronal apoptosis via Mas/PKA/CREB/UCP-2 pathway after subarachnoid hemorrhage in rats
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245 Open source automated western blot processor
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246 Slower development of PSI activity limits photosynthesis during Euonymus japonicus leaf development
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247 Effects of Angiotensin III on c-Jun N terminal Kinase in Wistar and Hypertensive Rat Vascular Smooth Muscle Cells
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248 Mitigation of Acetamiprid – Induced Renotoxicity by Natural Antioxidants via the Regulation of ICAM, NF-kB and TLR 4 pathways
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249 Dietary N-Carbamylglutamate (NCG) alleviates liver metabolic disease and hepatocyte apoptosis by suppressing ERK1/2-mTOR-S6K1 signal pathway via promoting endogenous arginine synthesis in Japanese seabass (Lateolabrax japonicus)
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250 a-Enolase as a novel vaccine candidate against Streptococcus dysgalactiae infection in cobia (Rachycentron canadum L.)
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251 Activation of TGR5 with INT-777 attenuates oxidative stress and neuronal apoptosis via cAMP/PKCe/ALDH2 pathway after subarachnoid hemorrhage in rats
Gang Zuo,Tongyu Zhang,Lei Huang,Camila Araujo,Jun Peng,Zachary Travis,Takeshi Okada,Umut Ocak,Guangyu Zhang,Jiping Tang,Xiaojun Lu,John H. Zhang
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252 Oral administration of 3,3'-diselenodipropionic acid prevents thoracic radiation induced pneumonitis in mice by suppressing NF-kB/IL-17/G-CSF/neutrophil axis
K.A. Gandhi,J.S. Goda,V.V. Gandhi,A. Sadanpurwala,V.K. Jain,K. Joshi,S. Epari,S. Rane,B. Mohanty,P. Chaudhari,S. Kembhavi,A. Kunwar,V. Gota,K.I. Priyadarsini
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253 Albumin fusion improves the pharmacokinetics and in vivo antitumor efficacy of canine interferon gamma
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254 In-silico and in-vitro analysis of endocan interaction with statins
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255 GlSir2.1 of Giardia lamblia is a NAD+-dependent cytoplasmic deacetylase
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256 Gene mining and identification of a flavone synthase II involved in flavones biosynthesis by transcriptomic analysis and targeted flavonoid profiling in Chrysanthemum indicum L.
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257 Cholesterol transporter proteins in HepG2 cells can be modulated by phenolic compounds present in Opuntia ficus-indica aqueous solutions
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258 Anti-diabetic properties of different fractions of Korean red ginseng
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259 Lipid metabolism disorders contribute to hepatotoxicity of triclosan in mice
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260 Protein biomarkers of neural system
Fatemeh Ghanavatinejad,Zahra Pourteymour Fard Tabrizi,Shadi Omidghaemi,Esmaeel Sharifi,Simon Geir Mřller,Mohammad-Saeid Jami
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261 Antigen retrieval by citrate solution improves western blot signal
Daniel Patińo-García,Nadia Rocha-Pérez,Ricardo D. Moreno,Renan Orellana
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262 The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis
Qing Zhao,Jun Yang,Meng-Ying Cui,Jie Liu,Yumin Fang,Mengxiao Yan,Wenqing Qiu,Huiwen Shang,Zhicheng Xu,Reheman Yidiresi,Jing-Ke Weng,Tomáš Pluskal,Marielle Vigouroux,Burkhard Steuernagel,Yukun Wei,Lei Yang,Yonghong Hu,Xiao-Ya Chen,Cathie Martin
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263 Inhibitors of Aß42-induced endoplasmic reticular unfolded protein response (UPRER), in yeast, also rescue yeast cells from Aß42-mediated apoptosis
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264 CYP enzymes, expressed within live human suspension cells, are superior to widely-used microsomal enzymes in identifying potent CYP1A1/CYP1B1 inhibitors: Identification of quinazolinones as CYP1A1/CYP1B1 inhibitors that efficiently reverse B[a]P toxicity and cisplatin resistance
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265 Genetic and epigenetic alterations induced by the small-molecule panobinostat: A mechanistic study at the chromosome and gene levels
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266 An alternative pathway for the formation of aromatic aroma compounds derived from l -phenylalanine via phenylpyruvic acid in tea ( Camellia sinensis (L.) O. Kuntze) leaves
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267 Anti-proliferative and cytotoxic activities of the flavonoid isoliquiritigenin in the human neuroblastoma cell line SH-SY5Y
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268 Gene isolation, heterologous expression, purification and functional confirmation of sesamin synthase from Sesamum indicum L.
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269 Cardiac glycoside Cerberin exerts anticancer activity through PI3K/AKT/mTOR signal transduction inhibition
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271 FGF-2 signaling activation in the hippocampus contributes to the behavioral and cellular responses to puerarin
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272 Evaluation of Renin and Soluble (Pro)renin Receptor in Patients with IPF. A Comparison with Hypersensitivity Pneumonitis
Montes Eduardo,Buendía-Roldan Ivette,Díaz-Pińa Gabriela,Moreno-Avila Veronica,Ruiz Victor
Lung. 2019;
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273 Aryl hydrocarbon receptor signaling, toxicity, and gene expression responses to mono-methylchrysenes
Ahmad Y. Alqassim,Mark J. Wilson,Jeffrey K. Wickliffe,Deepa Pangeni,Edward B. Overton,Charles A. Miller
Environmental Toxicology. 2019;
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274 Protective effects of Belamcandae Rhizoma against skin damage by ameliorating ultraviolet-B-induced apoptosis and collagen degradation in keratinocytes
Dongjin Noh,Jin Gyu Choi,Young Bae Lee,Young Pyo Jang,Myung Sook Oh
Environmental Toxicology. 2019;
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275 Human skin-derived ABCB5+ stem cell injection improves liver disease parameters in Mdr2KO mice
Vanessa Hartwig,Bedair Dewidar,Tao Lin,Anne Dropmann,Christoph Ganss,Mark Andreas Kluth,Nils Tappenbeck,Lysann Tietze,Bruno Christ,Markus Frank,Roger Vogelmann,Matthias Philip Alexander Ebert,Steven Dooley
Archives of Toxicology. 2019; 93(9): 2645
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276 A novel LAMP2 p.G93R mutation associated with mild Danon disease presenting with familial hypertrophic cardiomyopathy
Jing Xu,Lu Wang,Xiangdong Liu,Qiming Dai
Molecular Genetics & Genomic Medicine. 2019;
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277 Proteomic Detection and Validation of Translated Small Open Reading Frames
Alexandra Khitun,Sarah A. Slavoff
Current Protocols in Chemical Biology. 2019; 11(4)
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278 G Protein–Coupled Estrogen Receptor Production Using an Escherichia coli Cell-Free Expression System
Samson A. Souza,Dane T. Kurohara,Chester L. Dabalos,Ho Leung Ng
Current Protocols in Protein Science. 2019; 97(1)
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279 Functional characterization of host toxic EcdB transcription factor protein of echinocandin B biosynthetic gene cluster
Arvind Kumar,Vinay Kumar,Antresh Kumar
Biotechnology and Applied Biochemistry. 2019; 66(4): 626
[Pubmed] | [DOI]
280 Evaluation of the renoprotective effect of nano turmeric against toxic dose of copper sulfate: Role of vascular cell adhesion molecule-1, kidney injury molecule-1, and signal transducer and activator of transcription 3 protein expressions
Bshayer Alharbi,Laila Fadda,Hanaa M Ali
Journal of Biochemical and Molecular Toxicology. 2018; : e22243
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281 Effects of biphasic and monophasic electrical stimulation on mitochondrial dynamics, cell apoptosis, and cell proliferation
Maria R. Love,Jirapas Sripetchwandee,Siripong Palee,Siriporn C. Chattipakorn,Morton M. Mower,Nipon Chattipakorn
Journal of Cellular Physiology. 2018;
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282 Identification of a novel member of the pH responsive pathway Pal/Rim in Ustilago maydis
Juan A. Cervantes-Montelongo,José Ruiz-Herrera
Journal of Basic Microbiology. 2018;
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283 The protective mechanism of Nigella sativa against diethylnitrosamine-induced hepatocellular carcinoma through its antioxidant effect and EGFR/ERK1/2 signaling
Y. R. Shahin,N. M. Elguindy,A. Abdel Bary,M. Balbaa
Environmental Toxicology. 2018;
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284 The novel protective effects of loganin against 1-methyl-4-phenylpyridinium-induced neurotoxicity: Enhancement of neurotrophic signaling, activation of IGF-1R/GLP-1R , and inhibition of RhoA/ROCK pathway
Yu-Ting Tseng,Wan-Jung Lin,Wan-Hsuan Chang,Yi-Ching Lo
Phytotherapy Research. 2018;
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285 MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation
J. J. Brudvig,J. T. Cain,G. G. Schmidt-Grimminger,D. J. Stumpo,K. J. Roux,P. J. Blackshear,J. M. Weimer
Molecular Neurobiology. 2018;
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286 Early Diagnosis of Disease Using Microbead Array Technology: A Review
Atieh Vafajoo,Azin Rostami,Sanam Foroutan Parsa,Reza Salarian,Navid Rabiee,Ghazal Rabiee,Mohammad Rabiee,Mohammadreza Tahriri,Daryoosh Vashaee,Lobat Tayebi,Michael R. Hamblin
Analytica Chimica Acta. 2018;
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287 A Case of Concomitant Pemphigus Foliaceus and Oral Pemphigus Vulgaris
Alexandra C. Perks,Paula M. Farthing,Ruth Murphy,Anne M. Hegarty
Head and Neck Pathology. 2018;
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288 The role of posttranslational modifications of a-synuclein and LRRK2 in Parkinsonćs disease: Potential contributions of environmental factors
Edward Pajarillo,Asha Rizor,Jayden Lee,Michael Aschner,Eunsook Lee
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2018;
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289 5-Methoxyindole-2-carboxylic acid (MICA) suppresses Aß-mediated pathology in C. elegans
Waqar Ahmad,Paul R. Ebert
Experimental Gerontology. 2018; 108: 215
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290 Ameliorative role of ferulic acid against diabetes associated oxidative stress induced spleen damage
Sumit Ghosh,Sayantani Chowdhury,Poulami Sarkar,Parames C. Sil
Food and Chemical Toxicology. 2018; 118: 272
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291 Role of Dehydrin-FK506-binding proteins complex in enhancing drought tolerance through ABA-mediated signaling pathway
Poonam Tiwari,Yuvraj Indoliya,Pradyumna Kumar Singh,Poonam C. Singh,Puneet S. Chauhan,Veena Pande,Debasis Chakrabarty
Environmental and Experimental Botany. 2018;
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292 Divergent changes in the content and activity of MMP-26 and TIMP-4 in human umbilical cord tissues associated with preeclampsia
Lech Romanowicz,Tomasz Gogiel,Zofia Galewska,Marta Bruczko,Justyna Baczyk,Wieslawa Roszkowska-Jakimiec,Krzysztof Sobolewski
European Journal of Obstetrics & Gynecology and Reproductive Biology. 2018; 231: 48
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293 Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation
Hussaini Majiya,Oluwapelumi O. Adeyemi,Nicola J. Stonehouse,Paul Millner
Journal of Photochemistry and Photobiology B: Biology. 2018; 178: 404
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294 Expression, activation and processing of a novel plant milk-clotting aspartic protease in Pichia pastoris
Lucía Feijoo-Siota,José Luis R. Rama,Angeles Sánchez-Pérez,Tomás G. Villa
Journal of Biotechnology. 2018; 268: 28
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295 Identification of protective protein antigens for vaccination against Streptococcus dysgalactiae in cobia ( Rachycentron canadum )
Thuy Thi Thu Nguyen,Hai Trong Nguyen,Hung Vu-Khac,Pei-Chi Wang,Shih-Chu Chen
Fish & Shellfish Immunology. 2018; 80: 88
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296 Differential antiviral activity of European sea bass interferon-stimulated 15 protein (ISG15) against RGNNV and SJNNV betanodaviruses
Patricia Moreno,Daniel Alvarez-Torres,Esther Garcia-Rosado,Juan J. Borrego,M. Carmen Alonso
Fish & Shellfish Immunology. 2018; 83: 148
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297 Comparison of untagged and his-tagged dihydrodipicolinate synthase from the enteric pathogen Vibrio cholerae
Ruchi Gupta,Tatiana P. Soares da Costa,Pierre Faou,Con Dogovski,Matthew A. Perugini
Protein Expression and Purification. 2018;
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298 Chemi- and Bioluminescence of Cyclic Peroxides
Morgane Vacher,Ignacio Fdez. Galván,Bo-Wen Ding,Stefan Schramm,Romain Berraud-Pache,Pance Naumov,Nicolas Ferré,Ya-Jun Liu,Isabelle Navizet,Daniel Roca-Sanjuán,Wilhelm J. Baader,Roland Lindh
Chemical Reviews. 2018;
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299 Highly Sensitive and Multiplexed Protein Measurements
Limor Cohen,David R. Walt
Chemical Reviews. 2018;
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300 Plasmonic biosensor based on vertical arrays of gold nanoantennas
Stephanie Klinghammer,Tino Uhlig,Fabian Patrovsky,Matthias Böhm,Julian Schuett,Nils Pütz,Larysa Baraban,Lukas M. Eng,Gianaurelio Cuniberti
ACS Sensors. 2018;
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301 A non-canonical GTPase interaction enables ORP1L-Rab7-RILP complex formation and late endosome positioning
Xinli Ma,Kai Liu,Jian Li,Huanhuan Li,Jun Li,Yingfang Liu,Chonglin Yang,Huanhuan Liang
Journal of Biological Chemistry. 2018; 293(36): 14155
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302 Microchannel device for proteomic analysis of migrating cancer cells
Loan Bui,Qionghua Shen,Tamara Hill,Sayem H Bhuiyan,Rami Barakat,Vanessa Saavedra,Calvin Kong,James D Battiste,Young-tae Kim
Biomedical Physics & Engineering Express. 2018; 4(6): 065026
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303 Evaluation methods as quality control in the generation of decellularized peripheral nerve allografts
Charlot Philips,Maria Cornelissen,Víctor Carriel
Journal of Neural Engineering. 2018; 15(2): 021003
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304 Analysis for Science Librarians of the 2017 Nobel Prize in Physiology or Medicine: The Life and Work of Jeffrey C. Hall, Michael Rosbash, and Michael W. Young
Neyda V. Gilman
Science & Technology Libraries. 2018; : 1
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305 Effect of TAK1 on osteogenic differentiation of mesenchymal stem cells by regulating BMP-2 via Wnt/ß-catenin and MAPK pathway
Hongpeng Yang,Yue Guo,Dawei Wang,Xiaofei Yang,Chengzhi Ha
Organogenesis. 2018; 14(1): 36
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306 An ice-binding and tandem beta-sandwich domain-containing protein in Shewanella frigidimarina is a potential new type of ice adhesin
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The FEBS Journal. 2018;
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307 Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
Milena Radakovic,Suncica Borozan,Ninoslav Djelic,Saša Ivanovic,Dejana Cupic Miladinovic,Marko Ristanic,Biljana Spremo-Potparevic,Zoran Stanimirovic
Oxidative Medicine and Cellular Longevity. 2018; 2018: 1
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308 Leptin Modifies the Rat Heart Performance Associated with Mitochondrial Dysfunction Independently of Its Prohypertrophic Effects
Edna Berzabá-Evoli,Cecilia Zazueta,Jarumi Hishel Cruz Hernández,Nancy Patricia Gómez-Crisóstomo,Isela Esther Juárez-Rojop,Erick Natividad De la Cruz-Hernández,Eduardo Martínez-Abundis
International Journal of Endocrinology. 2018; 2018: 1
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309 Comparison of iTRAQ and SWATH in a clinical study with multiple time points
Antti Jylhä,Janika Nättinen,Ulla Aapola,Alexandra Mikhailova,Matti Nykter,Lei Zhou,Roger Beuerman,Hannu Uusitalo
Clinical Proteomics. 2018; 15(1)
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310 Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly
Oluwapelumi O. Adeyemi,Lee Sherry,Joseph C. Ward,Danielle M. Pierce,Morgan R. Herod,David J. Rowlands,Nicola J. Stonehouse,Julie K. Pfeiffer
Journal of Virology. 2018; 93(5)
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311 A Proteomics and other Omics approach in the context of farmed fish welfare and biomarker discovery
Cláudia Sofia Ferreira Raposo de Magalhăes,Marco Alexandre Cavaco Cerqueira,Denise Schrama,Márcio Júlio Vicente Moreira,Surintorn Boonanuntanasarn,Pedro Miguel Leal Rodrigues
Reviews in Aquaculture. 2018;
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312 Zinc stimulates glucose oxidation and glycemic control by modulating the insulin signaling pathway in human and mouse skeletal muscle cell lines
Shaghayegh Norouzi,John Adulcikas,Sukhwinder Singh Sohal,Stephen Myers,Chih-Hsin Tang
PLOS ONE. 2018; 13(1): e0191727
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313 The eNOS-NO pathway attenuates kidney dysfunction via suppression of inflammasome activation in aldosterone-induced renal injury model mice
Yuji Sogawa,Hajime Nagasu,Seiji Itano,Kengo Kidokoro,Shun’ichiro Taniguchi,Masafumi Takahashi,Hiroyuki Kadoya,Minoru Satoh,Tamaki Sasaki,Naoki Kashihara,David Long
PLOS ONE. 2018; 13(10): e0203823
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314 Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations
Lucas Moreira dos Santos,Carolina Georg Magalhăes,Paula de Lima Telmo,Michele Pepe Cerqueira,Rafael Amaral Donassolo,Fábio Pereira Leivas Leite,Guita Rubinsky Elefant,Luciana Farias da Costa Avila,Carlos James Scaini,Ângela Nunes Moreira,Fabricio Rochedo Conceiçăo,Paulo Lee Ho
PLOS ONE. 2018; 13(12): e0208991
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315 High SMAD7 and p-SMAD2,3 expression is associated with environmental enteropathy in children
Sana Syed,Vincenzo Dinallo,Najeeha T. Iqbal,Laura Di Iorio,Davide Di Fusco,Shan Guleria,Beatrice C. Amadi,Kamran Sadiq,Christopher Moskaluk,S. Asad Ali,Paul Kelly,Giovanni Monteleone,Joseph M. Vinetz
PLOS Neglected Tropical Diseases. 2018; 12(2): e0006224
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316 Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase
Neval Yaman Görük,Engin Deveci
Acta Cirurgica Brasileira. 2018; 33(7): 629
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317 Memeli Tümör ve Normal Hücre Hatlarinda Nanopartikül Uygulamalari
Yesim Daglioglu,Huri Özkan Yilmaz,Orhan Yilmaz
Arsiv Kaynak Tarama Dergisi. 2018; 27(2): 136
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318 Cystatin A suppresses tumor cell growth through inhibiting epithelial to mesenchymal transition in human lung cancer
Yunxia Ma,Yuan Chen,Yong Li,Katja Grün,Alexander Berndt,Zhongwei Zhou,Iver Petersen
Oncotarget. 2018; 9(18): 14084
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319 Cryoablation inhibition of distant untreated tumors (abscopal effect) is immune mediated
Xueling Yang,Yongfei Guo,Zhi Guo,Tongguo Si,Wenge Xing,Wenwen Yu,Yan Wang
Oncotarget. 2018; 10(41): 4180
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320 How to make P-glycoprotein (ABCB1, MDR1) harbor mutations and measure its expression and activity in cell cultures?
Pablo Zubiaur,Miriam Saiz-Rodríguez,Dora Koller,María Carmen Ovejero-Benito,Aneta Wojnicz,Francisco Abad-Santos
Pharmacogenomics. 2018;
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321 Padlock Probe Assay for Detection and Subtyping of Seasonal Influenza
Felix Neumann,Iván Hernández-Neuta,Malin Grabbe,Narayanan Madaboosi,Jan Albert,Mats Nilsson
Clinical Chemistry. 2018; 64(12): 1704
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322 Cell-Based Methods for Determination of Efficacy for Candidate Therapeutics in the Clinical Management of Cancer
Jenna Gordon,Mark Brown,Melissa Reynolds
Diseases. 2018; 6(4): 85
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323 Assessment of TSPO in a Rat Experimental Autoimmune Myocarditis Model: A Comparison Study between [18F]Fluoromethyl-PBR28 and [18F]CB251
Ga Kim,Jin Paeng,Jae Jung,Byung Moon,Antonio Lopalco,Nunzio Denora,Byung Lee,Sang Kim
International Journal of Molecular Sciences. 2018; 19(1): 276
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324 Oxidative Stress-Protective and Anti-Melanogenic Effects of Loliolide and Ethanol Extract from Fresh Water Green Algae, Prasiola japonica
Sang Park,Eunju Choi,Sunggyu Kim,Dong Kim,Ji Kim,SeokGu Chang,Jae Choi,Kyung Park,Kyung-Baeg Roh,Jongsung Lee,Byong Yoo,Jae Cho
International Journal of Molecular Sciences. 2018; 19(9): 2825
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325 Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing
Daniel Hauser,Manuela Estermann,Ana Milosevic,Lukas Steinmetz,Dimitri Vanhecke,Dedy Septiadi,Barbara Drasler,Alke Petri-Fink,Vincent Ball,Barbara Rothen-Rutishauser
Nanomaterials. 2018; 8(12): 1065
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326 Silencing of uPAR via RNA interference inhibits invasion and migration of oral tongue squamous cell carcinoma
Xuexiang Gao,Qi Guo,Shuo Wang,Cen Gao,Jian Chen,Li Zhang,Yuan Zhao,Jing Wang
Oncology Letters. 2018;
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327 Impact of Mutations in the Hemagglutinin of H10N7 Viruses Isolated from Seals on Virus Replication in Avian and Human Cells
Viruses. 2018; 10(2): 83
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328 Analytical techniques for characterization of biological molecules – proteins and aptamers/oligonucleotides
Harleen Kaur,Sonali R Bhagwat,Tarun Kumar Sharma,Amit Kumar
Bioanalysis. 2018;
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329 ??????????????? ???????? ??????????????? ??????????????? ????????????? ??????? ??? ?????? ??? ???????? ?????? ?????????????? ????????, "?????????"
?. ?. ???,?.?. ????????,?. ?. ????????,?.?. ?????????,?.?. ??????
Tsitologiya. 2018; (9): 704
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330 Development and application of an enzyme-linked immunosorbent assay (ELISA) using a soluble recombinant nucleoprotein for the detection of antibodies to avian influenza virus
Monezi Borzi Mariana,Rodrigues Silva Ketherson,de Fatima Silva Montassier Maria,Santos Fernando Filipe,de Lourdes Feres Tamanine Maria,Moreira dos Santos Romeu,Schirato de Oliveira Elisabete,Casagrande Mariguela Viviane,Diniz Lopes Priscila,Reischak Dilmara,Oliveira Mendonca Andre,Luis Luciano Renato,Jose Montassier Helio
African Journal of Microbiology Research. 2017; 11(18): 697
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331 Capsanthin Inhibits both Adipogenesis in 3T3-L1 Preadipocytes and Weight Gain in High-Fat Diet-Induced Obese Mice
Sung Jun Jo,Jeung Won Kim,Hye Ok Choi,Jung Hwan Kim,Hyung Joong Kim,Sun Hee Woo,Byung Hoon Han
Biomolecules & Therapeutics. 2017; 25(3): 329
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332 Bloch Surface Waves Biosensors for High Sensitivity Detection of Soluble ERBB2 in a Complex Biological Environment
Alberto Sinibaldi,Camilla Sampaoli,Norbert Danz,Peter Munzert,Frank Sonntag,Fabio Centola,Agostino Occhicone,Elisa Tremante,Patrizio Giacomini,Francesco Michelotti
Biosensors. 2017; 7(3): 33
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333 A Novel Egr-1-Agrin Pathway and Potential Implications for Regulation of Synaptic Physiology and Homeostasis at the Neuromuscular Junction
Ryen MacDonald,Sebastien Barbat-Artigas,Chulmin Cho,Huashan Peng,Jijun Shang,Ayman Moustaine,Salvatore Carbonetto,Richard Robitaille,Lorraine E. Chalifour,Hemant Paudel
Frontiers in Aging Neuroscience. 2017; 9
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334 Attenuation of the Infiltration of Angiotensin II Expressing CD3+ T-Cells and the Modulation of Nerve Growth Factor in Lumbar Dorsal Root Ganglia – A Possible Mechanism Underpinning Analgesia Produced by EMA300, An Angiotensin II Type 2 (AT2) Receptor Antagonist
Nemat Khan,Arjun Muralidharan,Maree T. Smith
Frontiers in Molecular Neuroscience. 2017; 10
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335 Toxin-Antitoxin Systems in Estuarine Synechococcus Strain CB0101 and Their Transcriptomic Responses to Environmental Stressors
David Marsan,Allen Place,Daniel Fucich,Feng Chen
Frontiers in Microbiology. 2017; 8
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336 A novel mouse model of anterior segment dysgenesis (ASD): conditional deletion ofTsc1disrupts ciliary body and iris development
Anna-Carin Hägglund,Iwan Jones,Leif Carlsson
Disease Models & Mechanisms. 2017; 10(3): 245
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337 Processing fixed and stored adipose-derived stem cells for quantitative protein array assays
Jessica S. Sadick,Eric M. Darling
BioTechniques. 2017; 63(6)
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338 Short-term regular aerobic exercise reduces oxidative stress produced by acute high intraluminal pressure in the adipose microvasculature
Austin T. Robinson,Ibra S. Fancher,Varadarajan Sudhahar,Jing Tan Bian,Marc D. Cook,Abeer M. Mahmoud,Mohamed M. Ali,Masuko Ushio-Fukai,Michael D. Brown,Tohru Fukai,Shane A. Phillips
American Journal of Physiology-Heart and Circulatory Physiology. 2017; 312(5): H896
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339 Semi-Automatic Analysis for Unidimensional Immunoblot Images to Discriminate Breast Cancer Cases Using Time Series Data Mining
Diana M. Sánchez-Silva,Héctor G. Acosta-Mesa,Tania Romo-González
International Journal of Pattern Recognition and Artificial Intelligence. 2017; : 1860004
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340 Discovery of a Novel Small-Molecule Inhibitor that Targets PP2A–ß-Catenin Signaling and Restricts Tumor Growth and Metastasis
Shrankhla Maheshwari,Srinivasa R. Avula,Akhilesh Singh,L. Ravithej Singh,Gopala R. Palnati,Rakesh K. Arya,Srikanth H. Cheruvu,Sudhir Shahi,Tanuj Sharma,Sanjeev Meena,Anup K. Singh,Ruchir Kant,Mohammed Riyazuddin,Himangsu K. Bora,Mohammad I. Siddiqi,Jiaur R. Gayen,Koneni V. Sashidhara,Dipak Datta
Molecular Cancer Therapeutics. 2017; 16(9): 1791
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341 5-hydroxytryptamine1A receptor density in the hippocampus of patients with temporal lobe epilepsy is associated with disease duration
N. C. Fonseca,H. P. G. Joaquim,L. L. Talib,S. Vincentiis,W. F. Gattaz,K. D. Valente
European Journal of Neurology. 2017;
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342 Deoxycholic acid promotes development of gastroesophageal reflux disease and Barrettćs oesophagus by modulating integrin-av trafficking
David O. Prichard,Anne Marie Byrne,James O. Murphy,John V. Reynolds,Jacintha OćSullivan,Ronan Feighery,Brendan Doyle,Osama Sharaf Eldin,Stephen P. Finn,Aoife Maguire,Deirdre Duff,Dermot P. Kelleher,Aideen Long
Journal of Cellular and Molecular Medicine. 2017;
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343 DNA polymerase beta participates in DNA End-joining
Sreerupa Ray,Gregory Breuer,Michelle DeVeaux,Daniel Zelterman,Ranjit Bindra,Joann B. Sweasy
Nucleic Acids Research. 2017;
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344 Proteomics and irritable bowel syndrome
Athanasios Tsigaridas,Ioannis S. Papanikolaou,Anna Vaiopoulou,Athanasios K. Anagnostopoulos,Nikos Viazis,George Karamanolis,Dimitrios G. Karamanolis,George T. Tsangaris,Gerasimos J. Mantzaris,Maria Gazouli
Expert Review of Proteomics. 2017; : 1
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345 L- and T-type calcium channel blockers protect against the inhibitory effects of mipafox on neurite outgrowth and plasticity-related proteins in SH-SY5Y cells
Laís Silva Fernandes,Neife Aparecida G. Dos Santos,Guilherme Luz Emerick,Antonio Cardozo Dos Santos
Journal of Toxicology and Environmental Health, Part A. 2017; : 1
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346 Fruit Peel Polyphenolic Extract-Induced Apoptosis in Human Breast Cancer Cells Is Associated with ROS Production and Modulation of p38MAPK/Erk1/2 and the Akt Signaling Pathway
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Nutrition and Cancer. 2017; : 1
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347 Cigarette smokers develop altered erythrocyte membrane composition: an investigation unmasking the role of membrane bound integral protein GLUT 1
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Free Radical Research. 2017; 51(4): 375
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348 Telmisartan Activates PPARd to Improve Symptoms of Unpredictable Chronic Mild Stress-Induced Depression in Mice
Yingxiao Li,Kai-Chun Cheng,Keng-Fan Liu,Wen-Huang Peng,Juei-Tang Cheng,Ho-Shan Niu
Scientific Reports. 2017; 7(1)
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349 The ENU-3 protein family members function in the Wnt pathway parallel to UNC-6/Netrin to promote motor neuron axon outgrowth in C. elegans
Roxana Oriana Florica,Victoria Hipolito,Stephen Bautista,Homa Anvari,Chloe Rapp,Suzan El-Rass,Alimohammad Asgharian,Costin N. Antonescu,Marie T. Killeen
Developmental Biology. 2017;
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350 Expression of Aspergillus Niger N5-5 in E. coli and Purification and Identification of Products
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Saudi Journal of Biological Sciences. 2017;
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351 High concentration of trichlorfon (1mM) disrupts axonal cytoskeleton and decreases the expression of plasticity-related proteins in SH-SY5Y cells
Laís Silva Fernandes,Guilherme Luz Emerick,Rafaela Scalco Ferreira,Neife Aparecida G. dos Santos,Antonio Cardozo dos Santos
Toxicology in Vitro. 2017; 39: 84
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352 Hexacosanol reduces plasma and hepatic cholesterol by activation of AMP-activated protein kinase and suppression of sterol regulatory element-binding protein-2 in HepG2 and C57BL/6J mice
Ji Hae Lee,Yaoyao Jia,Trung Thanh Thach,Yuri Han,Bobae Kim,Chunyan Wu,Yeonji Kim,Woo Duck Seo,Sung-Joon Lee
Nutrition Research. 2017;
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353 Protective Effects of ?-Mangostin on 6-OHDA-Induced Toxicity in SH-SY5Y Cells
Yamaratee Jaisin,Piyanee Ratanachamnong,Chitraporn Kuanpradit,Watinee Khumpum,Sunit Suksamrarn
Neuroscience Letters. 2017;
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354 Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation
Ramirez-Lee Manuel Alejandro,Martinez-Cuevas Pedro Pablo,Rosas-Hernandez Hector,Oros-Ovalle Cuauhtémoc,Bravo-Sanchez Mariela,Martinez-Castańon Gabriel Alejandro,Gonzalez Carmen
Nanomedicine: Nanotechnology, Biology and Medicine. 2017;
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355 Silencing of the pentose phosphate pathway genes influences DNA replication in human fibroblasts
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359 Human Sirtuin 3 (SIRT3) deacetylates histone H3 lysine 56 to promote Nonhomologous end joining repair
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360 Altered functional efficacy of hippocampal interneuron during epileptogenesis following febrile seizures
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363 Immunomodulatory and therapeutic role of Cinnamomum verum extracts in collagen-induced arthritic BALB/c mice
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364 Quantitative proteome-based systematic identification of SIRT7 substrates
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365 Using SDS-PAGE gel fingerprinting to identify soft-bodied wood-boring insect larvae to species
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366 Combining Cytotoxicity Assessment and Xenopus laevis Phenotypic Abnormality Assay as a Predictor of Nanomaterial Safety
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367 Influence of Clinically Relevant Mechanical Forces on Vascular Smooth Muscle Cells Under Chronic High Glucose: An In Vitro Dynamic Disease Model
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368 ING3 is associated with increased cell invasion and lethal outcome in ERG-negative prostate cancer patients
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370 Atorvastatin improves pathological changes in the aged kidney by upregulating peroxisome proliferator-activated receptor expression and reducing matrix metalloproteinase-9 and transforming growth factor-ß1 levels
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371 Perturbation of redox balance after thioredoxin reductase deficiency interrupts autophagy-lysosomal degradation pathway and enhances cell death in nutritionally stressed SH-SY5Y cells
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372 Sphingosine kinase inhibition ameliorates chronic hypoperfusion-induced white matter lesions
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373 Characterization and functional analysis of fatty acid binding protein from the carcinogenic liver fluke, Opisthorchis viverrini
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374 Anti-diabetes and anti-obesity: A meta-analysis of different compounds
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375 Easy and Fast Western Blotting by Thin-Film Direct Coating with Suction
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376 Protein characterization of intracellular target-sorted, formalin-fixed cell subpopulations
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377 Quantification of Confocal Images Using LabVIEW for Tissue Engineering Applications
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378 An overview of technical considerations for Western blotting applications to physiological research
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379 Minimising invasiveness in diagnostics: developing a rapid urine-based monoclonal antibody dipstick test for malaria
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380 Affinity Pulldown of Biotinylated RNA for Detection of Protein-RNA Complexes
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381 Characterization of Streptococcus sanguis molecular receptors for Streptococcus mutans binding molecules
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382 Neuroprotective Effect of Puerarin on Glutamate-Induced Cytotoxicity in Differentiated Y-79 Cells via Inhibition of ROS Generation and Ca2+ Influx
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383 The Potentials and Pitfalls of Microarrays in Neglected Tropical Diseases: A Focus on Human Filarial Infections
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384 Monocyte Chemoattractant Protein-Induced Protein 1 (MCPIP1) Enhances Angiogenic and Cardiomyogenic Potential of Murine Bone Marrow-Derived Mesenchymal Stem Cells
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386 Considerations when quantitating protein abundance by immunoblot
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388 Patient Mutation Directed shRNA Screen Uncovers Novel Bladder Tumor Growth Suppressors
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389 Increased HMGB1 and cleaved caspase-3 stimulate the proliferation of tumor cells and are correlated with the poor prognosis in colorectal cancer
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390 A novel mouse model of tuberous sclerosis complex (TSC): eye-specific Tsc1-ablation disrupts visual-pathway development
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391 A combination of high dose rate (10X FFF/2400?MU/min/10?MV X-rays) and total low dose (0.5?Gy) induces a higher rate of apoptosis in melanoma cells in vitro and superior preservation of normal melanocytes
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392 The Localization of Cytochrome P450s CYP1A1 and CYP1A2 into Different Lipid Microdomains Is Governed by Their N-terminal and Internal Protein Regions
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393 A DEVH-box RNA Helicase from Leishmania braziliensis is Associated to mRNA Cytoplasmic Granules
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394 Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation
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395 In Vitro Analysis of the Very-Low Density Lipoprotein Export from the Trans -Golgi Network
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396 Inhibition of connective tissue growth factor attenuates paraquat-induced lung fibrosis in a human MRC-5 cell line
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397 Aspalathin improves glucose and lipid metabolism in 3T3-L1 adipocytes exposed to palmitate
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398 Cloning of the first human anti-JCPyV/VP1 neutralizing monoclonal antibody: epitope definition and implications in risk stratification of patients under natalizumab therapy
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399 The future of whole-cell modeling
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400 Different Effects of antimitotic drug on mechanical behaviors of Cancer cytoskeleton: Investigating distinct Grades of "colon Cancer cell lines"
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405 Cholera toxin suppresses expression of ubiquitin editing enzyme A20 and enhances transcytosis
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