| TECHNICAL ARTICLE |
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| Year : 2012 | Volume
: 4
| Issue : 9 | Page : 429-434 |
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Western blot: Technique, theory, and trouble shooting
Tahrin Mahmood, Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada
Correspondence Address:
Ping-Chang Yang
Department of Pathology & Molecular Medicine, McMaster University, Room T3303, 50 Charlton Ave East, Hamilton, ON
Canada
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/1947-2714.100998
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Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot. |
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