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RESEARCH LETTER
Year : 2014  |  Volume : 6  |  Issue : 5  |  Page : 231-233

DNA extraction from nocardia species for special genes analysis using PCR


1 Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Bacteriology and Virology, Alborz University of Medical Sciences, Karaj, Iran

Correspondence Address:
Seyyed Saeed Eshraghi
Department of Microbiology, Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1947-2714.132943

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Background : Nocardia species have a complex cell wall structure similar to that of mycobacteria, and the extraction of DNA from this bacterium is extremely diffi cult. Currently, to identify Nocardia species particularly, it is essential to utilize molecular techniques. Aims : In the present study, we investigated STET (sodium chloride-TRIS-EDTA-triton) buffer for the extraction of high-quality genomic DNA from 20 clinical and environmental isolates. Materials and Methods: The extracted DNA was evaluated for portion of the 16S rRNA, 65-kDa heat-shock protein and 16S rRNA genes via polymerase chain reaction. Results: The extracted DNA had high molecular mass, and its concentration and purity was suitable when tested in 1% agarose gel, and using UV spectrophotometry. Amplifi cation of three different genes was successfully performed. Conclusion: This paper reveals an inexpensive, reproducible and effi cient method of DNA extraction from Nocardia species, which is appropriate for accurate identifi cation of this bacterium via polymerase chain reaction and polymerase chain reaction-restriction fragment length polymorphism.


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