North American Journal of Medical Sciences

TECHNICAL ARTICLE
Year
: 2012  |  Volume : 4  |  Issue : 12  |  Page : 651--655

Construction of pET-32 α (+) vector for protein expression and purification


Zhi-Qiang Liu, Ping-Chang Yang 
 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON
Canada

The construction of expression vector is a basic tool for biotechnology and production of desired proteins, this article summarized the construction of pET-32 α (+) vector techniques which are generally used in research laboratories. The procedures include that acquisition of the exogenous DNA fragment for construction of the vector, subcloning the DNA fragment into pET-32 α (+) expression vector, protein expression in Escherichia coli BL21 (DE3) and protein purification under native conditions in E. coli lysates.


How to cite this article:
Liu ZQ, Yang PC. Construction of pET-32 α (+) vector for protein expression and purification.North Am J Med Sci 2012;4:651-655


How to cite this URL:
Liu ZQ, Yang PC. Construction of pET-32 α (+) vector for protein expression and purification. North Am J Med Sci [serial online] 2012 [cited 2021 Apr 13 ];4:651-655
Available from: https://www.najms.org/article.asp?issn=1947-2714;year=2012;volume=4;issue=12;spage=651;epage=655;aulast=Liu;type=0