North American Journal of Medical Sciences

TECHNICAL ARTICLE
Year
: 2012  |  Volume : 4  |  Issue : 9  |  Page : 429--434

Western blot: Technique, theory, and trouble shooting


Tahrin Mahmood, Ping-Chang Yang 
 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, Canada

Correspondence Address:
Ping-Chang Yang
Department of Pathology & Molecular Medicine, McMaster University, Room T3303, 50 Charlton Ave East, Hamilton, ON
Canada

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. This paper will attempt to explain the technique and theory behind western blot, and offer some ways to troubleshoot.


How to cite this article:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting.North Am J Med Sci 2012;4:429-434


How to cite this URL:
Mahmood T, Yang PC. Western blot: Technique, theory, and trouble shooting. North Am J Med Sci [serial online] 2012 [cited 2021 Dec 9 ];4:429-434
Available from: https://www.najms.org/article.asp?issn=1947-2714;year=2012;volume=4;issue=9;spage=429;epage=434;aulast=Mahmood;type=0