North American Journal of Medical Sciences

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 7  |  Issue : 2  |  Page : 59--64

NG -nitro-L-arginine methyl ester protects against hormonal imbalances associated with nicotine administration in male rats


Ibukun P Oyeyipo1, Y Raji2, Adeyombo F Bolarinwa2 
1 Department of Physiology, College of Health Sciences, Osun State University, Osogbo, Osun, Nigeria; Department of Biomedical Sciences, Division of Medical Physiology, Stellenbosch University, Tygerberg, South Africa
2 Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Oyo, Nigeria

Correspondence Address:
Ibukun P Oyeyipo
Department of Physiology, College of Health Sciences, Osun State University, Osogbo, Osun, Nigeria

Background: The administration of nicotine is associated with altered hormonal imbalances and increased serum and testicular nitric oxide (NO) level. Aim: This study sought to investigate the effects of NO inhibition with NG -nitro-L-arginine methyl ester (L-NAME) on altered hormonal imbalance in adult male albinorats. Materials and Methods: Rats were administered with 0.5 mg/kg body weight (BW) and 1.0 mg/kg BW nicotine and were treated with L-NAME in the drinking water or drinking water alone for 30 days. Serum was analyzed for testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH), and prolactin using radioimmunoassay. Results: Nicotine administration significantly decreased (P < 0.05) testosterone in the low and high dose treated groups and FSH in the high dose treated group when compared with the control group. There was a significant increase (P < 0.05) in mean LH and prolactin level in the high dose treated group when compared with the control. Concomitant treatment with nicotine and L-NAME produced significant increases in testosterone and FSH, and a decrease in prolactin in 1.0 mg/kg BW. L-NAME alone did not lead to a significant increase in testosterone when compared with control. Conclusion: These data demonstrate that the suppressive effects of nicotine on testosterone level of the adult male rat can be prevented by NOS blockade with L-NAME. It appears that these beneficial effects are mediated primarily within the gonad; however, the involvement of the pituitary cannot be totally ruled out.


How to cite this article:
Oyeyipo IP, Raji Y, Bolarinwa AF. NG -nitro-L-arginine methyl ester protects against hormonal imbalances associated with nicotine administration in male rats.North Am J Med Sci 2015;7:59-64


How to cite this URL:
Oyeyipo IP, Raji Y, Bolarinwa AF. NG -nitro-L-arginine methyl ester protects against hormonal imbalances associated with nicotine administration in male rats. North Am J Med Sci [serial online] 2015 [cited 2021 Oct 21 ];7:59-64
Available from: https://www.najms.org/article.asp?issn=1947-2714;year=2015;volume=7;issue=2;spage=59;epage=64;aulast=Oyeyipo;type=0