North American Journal of Medical Sciences

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 7  |  Issue : 6  |  Page : 253--258

Respiratory burst enzymes, pro-oxidants and antioxidants status in Bangladeshi population with β-thalassemia major


Md. Faruk Hossain, Md. Ismail, Arifur Rahman Tanu, Hossain Uddin Shekhar 
 Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh

Correspondence Address:
Dr. Hossain Uddin Shekhar
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka - 1000
Bangladesh

Background: Oxidative stress is intimately associated with many diseases, including β-thalassemia. Aim: The study was to estimate the status of respiratory burst enzymes, pro-oxidants, and antioxidants in β-thalassemia major patients in Bangladesh and to compare with apparently healthy individuals. Materials and Methods: A total of 49 subjects were recruited which included 25 patients (age range 5 to 40 years) with β-thalassemia major and 24 controls (age and sex matched). Superoxide dismutase (SOD) and catalase (CAT) represented respiratory burst enzymes; malondialdehyde (MDA), lipid hydroperoxide (LHP), and xanthine oxidase (XO) were measured as pro-oxidants; and glutathione S transferase (GST), vitamin C (Vit.C), and glutathione (GSH) were the measured antioxidants. Results: The activity of SOD was significantly (P < 0.001) increased by about 79% and the activity of CAT was significantly (P < 0.001) decreased by more than 34% in the blood of β-thalassemia major patients compared to the control group. The content of pro-oxidants such as MDA, LHP, and XO was significantly (P < 0.001) higher in patients by about 228%, 241.3% and 148.1% respectively compared to control group. The level of GSH and Vit.C were significantly (P = 0.000) decreased in patients by about 59% and 81% versus the healthy group, respectively; and GST activity was significantly (P < 0.001) declined by 44.25% in patients group. Conclusion: β-thalassemia major patients demonstrate raised oxidative stress compared to healthy subjects.


How to cite this article:
Hossain M, Ismail M, Tanu AR, Shekhar HU. Respiratory burst enzymes, pro-oxidants and antioxidants status in Bangladeshi population with β-thalassemia major.North Am J Med Sci 2015;7:253-258


How to cite this URL:
Hossain M, Ismail M, Tanu AR, Shekhar HU. Respiratory burst enzymes, pro-oxidants and antioxidants status in Bangladeshi population with β-thalassemia major. North Am J Med Sci [serial online] 2015 [cited 2021 May 16 ];7:253-258
Available from: https://www.najms.org/article.asp?issn=1947-2714;year=2015;volume=7;issue=6;spage=253;epage=258;aulast=Hossain;type=0