North American Journal of Medical Sciences

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 7  |  Issue : 9  |  Page : 390--396

The preventive effects of neural stem cells and mesenchymal stem cells intra-ventricular injection on brain stroke in rats


Seyed Mojtaba Hosseini1, Nastaran Samimi2, Mohammad Farahmandnia2, Benafshe Shakibajahromi2, Fatemeh Sabet Sarvestani2, Mahsa Sani1, Masoomeh Mohamadpour4 
1 Students Research Committee; Cell and Molecular Medicine Student Research Group, Medical Faculty; Stem Cell Laboratory, Department of Anatomy, Shiraz University of Medical Sciences, Shiraz, Iran
2 Students Research Committee; Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran

Correspondence Address:
Seyed Mojtaba Hosseini
Department of Anatomy, Medical Faculty, Shiraz University of Medical Sciences, Zand Blvd, Shiraz
Iran

Introduction: Stroke is one of the most important causes of disability in developed countries and, unfortunately, there is no effective treatment for this major problem of central nervous system (CNS); cell therapy may be helpful to recover this disease. In some conditions such as cardiac surgeries and neurosurgeries, there are some possibilities of happening brain stroke. Inflammation of CNS plays an important role in stroke pathogenesis, in addition, apoptosis and neural death could be the other reasons of poor neurological out come after stroke. In this study, we examined the preventive effects of the neural stem cells (NSCs) and mesenchymal stem cells (MSCs) intra-ventricular injected on stroke in rats. Aim: The aim of this study was to investigate the preventive effects of neural and MSCs for stroke in rats. Materials and Methods: The MSCs were isolated by flashing the femurs and tibias of the male rats with appropriate media. The NSCs were isolated from rat embryo ganglion eminence and they cultured NSCs media till the neurospheres formed. Both NSCs and MSCs were labeled with PKH26-GL. One day before stroke, the cells were injected into lateral ventricle stereotactically. Results: During following for 28 days, the neurological scores indicated that there are better recoveries in the groups received stem cells and they had less lesion volume in their brain measured by hematoxylin and eosin staining. Furthermore, the activities of caspase-3 were lower in the stem cell received groups than control group and the florescent microscopy images showed that the stem cells migrated to various zones of the brains. Conclusion: Both NSCs and MSCs are capable of protecting the CNS against ischemia and they may be good ways to prevent brain stroke consequences situations.


How to cite this article:
Hosseini SM, Samimi N, Farahmandnia M, Shakibajahromi B, Sarvestani FS, Sani M, Mohamadpour M. The preventive effects of neural stem cells and mesenchymal stem cells intra-ventricular injection on brain stroke in rats.North Am J Med Sci 2015;7:390-396


How to cite this URL:
Hosseini SM, Samimi N, Farahmandnia M, Shakibajahromi B, Sarvestani FS, Sani M, Mohamadpour M. The preventive effects of neural stem cells and mesenchymal stem cells intra-ventricular injection on brain stroke in rats. North Am J Med Sci [serial online] 2015 [cited 2021 Oct 25 ];7:390-396
Available from: https://www.najms.org/article.asp?issn=1947-2714;year=2015;volume=7;issue=9;spage=390;epage=396;aulast=Hosseini;type=0