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  Indian J Med Microbiol
 

Figure 1: The protein concentration measurements using Lowry is more consistent than Bradford methods. The albumin, commonly used as standard for protein measurements, are prepared in serial dilutions (0ìg/ml, 2ìg/ml, 4ìg/ml, 6ìg/ml, 8ìg/ml and 10ìg/ml), in triplicates. The measurements were carried out using a time course of 30 minutes with 5 minutes intervals. (A) Absorbance change was calculated using the reference point of 0ìg/ml at time 0, with all other samples compared to this; this value was denoted Delta. Delta is < 0.01 at every sample preparation using Lowry method; while the Delta is increasing in using Bradford method (2ìg/ml: 0.007-0.013; 4ìg/ml: 0.004-0.002; 6ìg/ml: 0.012-0.04, 8ìg/ml: 0.008-0.052; and 10ìg/ml: 0.008-0.061). The X-axis in figure 1A represents 30-minutes time course on serial sample preparations; Lowry method (solid line) and Bradford method (dotted line). The Y-axis represents the Delta. (B) The Y-axis in figure 1B is protein concentration expressed in logarithmic 10. L: Lowry Method; B: Bradford Method.

Figure 1: The protein concentration measurements using Lowry is more consistent than Bradford methods. The albumin, commonly used as standard for protein measurements, are prepared in serial dilutions (0ìg/ml, 2ìg/ml, 4ìg/ml, 6ìg/ml, 8ìg/ml and 10ìg/ml), in triplicates. The measurements were carried out using a time course of 30 minutes with 5 minutes intervals. (A) Absorbance change was calculated using the reference point of 0ìg/ml at time 0, with all other samples compared to this; this value was denoted Delta. Delta is < 0.01 at every sample preparation using Lowry method; while the Delta is increasing in using Bradford method (2ìg/ml: 0.007-0.013; 4ìg/ml: 0.004-0.002; 6ìg/ml: 0.012-0.04, 8ìg/ml: 0.008-0.052; and 10ìg/ml: 0.008-0.061). The X-axis in figure 1A represents 30-minutes time course on serial sample preparations; Lowry method (solid line) and Bradford method (dotted line). The Y-axis represents the Delta. (B) The Y-axis in figure 1B is protein concentration expressed in logarithmic 10. L: Lowry Method; B: Bradford Method.