Figure 2: All DIF. 2a, positive staining for JAM-A in the area of the blister (white staining, white arrow). 2b, positive staining against JAM-A in several areas of the skin (white staining, white arrows).The yellow arrow shows positive staining against ezrin. 2c. Positive intercellular staining (ICS) between the basal keratinocytes (white arrow), and against the basement membrane zone (BMZ) of the skin (yellow arrow) using FITC conjugated anti-human IgG, possibly as results of the affinity of the herpes virus for the Fc portion of the immunoglobulins. ELISA studies for desmogleins 1 and 2 were both negative, as well as the immunoblotting on skin extracts. 2d Similar to 2c, but in this case with Complement/C3. 2 e, f and g, Show positive staining in red for ezrin (yellow arrows), against JAM-A (diffuse areas in white), (stars), and against FITC conjugated human IgG (greenish staining, white arrows). h, and i, Positive staining against ezrin (red staining, yellow arrows). The nuclei were counterstained with DAPI (blue).