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  Indian J Med Microbiol
 

Figure 3: (DIF in b, c and d, H& E in a, e, g and bleached H&E in h, IHC in f) . a, Some alterations in dermal sweat glands (black arrows), and their respective nerves (red arrows), under the area where the viral infection was detected in the epidermis. b . Positive staining of the sweat glands (white arrows, green staining) and the nerves (red arrows, green staining with FITC conjugated anti human IgG and C3 -FITC antibodies). c . The positive nerve, at higher magnification (green staining, red arrow). d . Similar to b and c , but in this case, to confirm the identity of the nerve, we co-localized the nerve by staining with Cy3 conjugated anti-human glial fibrillary acidic protein (GFAP)(white staining, red arrow). A nearby sweat gland is indicated by the white arrow. e . Some rarefaction of the sweat glands on H&E at higher magnification. f. IHC positive staining of Complement/C5-C9/MAC on sweat glands (brown staining; black arrow). g and h . Melanin bleaching confirmed the presence of melanin in those pigmented areas (H&E and melanin bleaching, respectively; black arrows). i Shows the clinical generalized vesicles and bullae on the upper arm (black arrow).

Figure 3: (DIF in b, c and d, H& E in a, e, g and bleached H&E in h, IHC in f) . a, Some alterations in dermal sweat glands (black arrows), and their respective nerves (red arrows), under the area where the viral infection was detected in the epidermis. b . Positive staining of the sweat glands (white arrows, green staining) and the nerves (red arrows, green staining with FITC conjugated anti human IgG and C3 -FITC antibodies). c . The positive nerve, at higher magnification (green staining, red arrow). d . Similar to b and c , but in this case, to confirm the identity of the nerve, we co-localized the nerve by staining with Cy3 conjugated anti-human glial fibrillary acidic protein (GFAP)(white staining, red arrow). A nearby sweat gland is indicated by the white arrow. e . Some rarefaction of the sweat glands on H&E at higher magnification. f. IHC positive staining of Complement/C5-C9/MAC on sweat glands (brown staining; black arrow). g and h . Melanin bleaching confirmed the presence of melanin in those pigmented areas (H&E and melanin bleaching, respectively; black arrows). i Shows the clinical generalized vesicles and bullae on the upper arm (black arrow).