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  Indian J Med Microbiol
 

Figure 6: SDS-PAGE analysis of the fusion protein induced by IPTG. Proteins were separated by SDS-PAGE and stained with Coomassie brilliant blue. Lane 1: protein molecular weight marker; Lane 2: supernatant after sonication of uninduced BL-21/pET32 a (+)/pTBO; Lane 3: precipitate after sonication of induced BL-21/ pET 32 a (+)/ pTBO; Lane 4: supernatant after sonication of BL-21/ pET 32 a (+)/ pTBO induced for 5 hours. The molecular weight of pTBO fusion protein is about 22 kDa

Figure 6: SDS-PAGE analysis of the fusion protein induced by IPTG. Proteins were separated by SDS-PAGE and stained with Coomassie brilliant blue. Lane 1: protein molecular weight marker; Lane 2: supernatant after sonication of uninduced BL-21/pET32 a (+)/pTBO; Lane 3: precipitate after sonication of induced BL-21/ pET 32 a (+)/ pTBO; Lane 4: supernatant after sonication of BL-21/ pET 32 a (+)/ pTBO induced for 5 hours. The molecular weight of pTBO fusion protein is about 22 kDa